A one-step cloning method for the construction of somatic cell gene targeting vectors: Application to production of human knockout cell lines

BMC Biotechnology

Volumn 12, Issue , 2012, Pages

  Liu, Yi ^a   Li, Shangze ^a   Zhang, Huihui ^a   Wan, Zurong ^a   Zhang, Xiaodong ^a   Du, Runlei ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ADENO-ASSOCIATED VIRUS; CELL LINES; COLORECTAL CANCER CELL; EXONUCLEASE; GENE TARGETING; GENOMIC DNA; HIGH-THROUGHPUT; KNOCK OUTS; ONE-STEP CLONING; RESTRICTION ENZYMES; SHUTTLE VECTOR; SOMATIC CELLS;

CELL CULTURE; CLONE CELLS; CLONING; DISEASES; ESCHERICHIA COLI; VIRUSES;

VECTORS;

ADENOVIRUS VECTOR; EXODEOXYRIBONUCLEASE III; HISTONE DEACETYLASE 2; SIRTUIN 1;

ARTICLE; CANCER CELL; CLONING; COLORECTAL CANCER; ESCHERICHIA COLI; GENE INACTIVATION; GENE VECTOR; NONHUMAN; POLYMERASE CHAIN REACTION; SOMATIC CELL GENETICS; SOMATIC GENE THERAPY;

CLONING, MOLECULAR; DEPENDOVIRUS; ESCHERICHIA COLI; EXODEOXYRIBONUCLEASES; GENE KNOCKOUT TECHNIQUES; GENE TARGETING; GENETIC VECTORS; HCT116 CELLS; HEK293 CELLS; HISTONE DEACETYLASE 2; HUMANS; SIRTUIN 1;

ADENO-ASSOCIATED VIRUS; ESCHERICHIA COLI;

EID: 84867123025     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-12-71     Document Type: Article

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