Probiotic supplementation affects markers of intestinal barrier, oxidation, and inflammation in trained men; a randomized, double-blinded, placebo-controlled trial

Journal of the International Society of Sports Nutrition

Volumn 9, Issue , 2012, Pages

  Lamprecht, Manfred ^a,b   Bogner, Simon ^a,b   Schippinger, Gert ^c   Steinbauer, Kurt ^c   Fankhauser, Florian ^c   Hallstroem, Seth ^a   Schuetz, Burkhard ^d   Greilberger, Joachim F ^a,e  

^a MEDICAL UNIVERSITY OF GRAZ   (Austria)

^b Institute of Nutrient Research and Sport Nutrition   (Austria)

^c SportchirurgiePlus   (Austria)

^d Biovis Diagnostik MVZ GmbH   (Germany)

^e Dr Greilberger GmbH   (Austria)

Author keywords

Intense exercise; Leaky gut; Oxidative stress and inflammation; Probiotics

Indexed keywords

TOSCANA VIRUS;

EID: 84867116816     PISSN: None     EISSN: 15502783     Source Type: Journal    
DOI: 10.1186/1550-2783-9-45     Document Type: Article

References (53)

1. Salminen S, Bouley D, Bourron-Ruault MC, Cummings JH, Franck A, Gibson GR, Isolauri E, Moreau MC, Roberfroid M, Rowland I. Functional food sciene and gastrointestinal physiology and function. Br J Nutr 1998, 80(Suppl):S147-S171.
2. Gleeson M, Bishop NC, Oliveira M, Tauler P. Daily probiotic's (Lactobacillus casei Shirota) reduction of infection incidence in athletes. Int J Sport Nutr Exerc Metab 2011, 21:55-64.
3. Cox AJ, Pyne DB, Saunders PU, Fricker PA. Oral administration of the probiotic Lactobacillus fermentum VRI-003 and mucosal immunity in endurance athletes. Br J Sports Med 2010, 44:222-226. 10.1136/bjsm.2007.044628, 18272539.
4. Kekkonen RA, Vasankari TJ, Vuorimaa T, Haahtela T, Julkunen I, Korpela R. The effect of probiotics on respiratory infections and gastrointestinal symptoms during training in marathon runners. Int J Sport Nutr Exerc Metab 2007, 17:352-363.
5. West NP, Pyne DB, Cripps AW, Hopkins WG, Eskesen DC, Jairath A, Christophersen CT, Conlon MA, Fricker PA. Lactobacillus fermentum (PCC®) supplementation and gastrointestinal and respiratory-tract illness symptoms: a randomised control trial in athlets. Nutr J 2011, 10:30. 10.1186/1475-2891-10-30, 3083335, 21477383.
6. Martarelli D, Verdenelli MC, Scuri S, Cocchioni M, Silvi S, Cecchini C, Pompei P. Effect of a probiotic intake on oxidant and antioxidant parameters in plasma of athletes during intense exercise training. Curr Microbiol 2011, 62:1689-1696. 10.1007/s00284-011-9915-3, 21400082.
7. Rehrer NJ, Brouns F, Beckers EJ, Frey WO, Villiger B, Riddoch CJ, Menheere PP, Saris WH. Physiological changes and gastro-intestinal symptoms as a result of ultra-endurance running. Eur J Appl Physiol Occup Physiol 1992, 64:1-8. 10.1007/BF00376431, 1735404.
8. Qarnar MI, Read AE. Effects of exercise on mesenteric blood flow in man. Gut 1987, 28:583-587. 10.1136/gut.28.5.583, 1432887, 3596339.
9. Lambert GP. Stress-induced gastrointestinal barrier dysfunction and ist inflammatory effects. J Anim Sci 2009, 87(E.Suppl):E101-E108.
10. West NP, Pyne DB, Peake JM, Cripps AW. Probiotics, immunity and exercise: a review. Exerc Immunol Rev 2009, 15:107-126.
11. Fasano A. Leaky gut and autoimmune diseases. Clinic Rev Allerg Immunol 2012, 42:71-78.
12. DeOliveira EP, Burini RC. Food-dependent, exercise-induced gastrointestinal distress. J Int Soc Sports Nutr 2011, 8:12. 10.1186/1550-2783-8-12, 3190328, 21955383.
13. Fasano A. Pathological and therapeutical implications of macro-molecule passage through the tight junction. Tight Junctions CRC Press, Boca Raton, Cereijido M, Anderson J, 2, 2001:697-722.
14. Ulluwishewa D, Anderson RC, McNabb WC, Moughan PJ, Wells JM, Roy NC. Regulation of tight junction permeability by intestinal bacteria and dietary components. J Nutr 2011, 141:769-776. 10.3945/jn.110.135657, 21430248.
15. Qin H, Zhang Z, Hang X, Jiang YL. L. plantarum prevents enteroinvasive Escherichia coli-induced tight junction proteins changes in intestinal epithelial cells. BMC Microbiol 2009, 9:63. 10.1186/1471-2180-9-63, 2674056, 19331693.
16. Anderson RC, Cookson AL, McNabb WC, Kelly WJ, Roy NC. Lactobacillus plantarum DSM 2648 is a potential probiotic that enhances intestinal barrier function. FEMS Microbiol Lett 2010, 309:184-192.
17. Karczewski J, Troost FJ, Konings I, Dekker J, Kleerebezem M, Brummer RJM, Wells JM. Regulation of human epithelial tight junction proteins by Lactobacillus plantarum in vivo and protective effects on the epithelial barrier. Am J Physiol Gastrointest Liver Physiol 2010, 298:G851-G859. 10.1152/ajpgi.00327.2009, 20224007.
18. Resta-Lenert S, Barrett KE. Probiotics and commensals reverse TNF-alpha- and IFN-gamma-induced dysfunction in human intestinal epithelial cells. Gastroenterology 2006, 130:731-746. 10.1053/j.gastro.2005.12.015, 16530515.
19. Ukena SN, Singh A, Dringenberg U, Engelhardt R, Seidler U, Hansen W, Bleich A, Bruder D, Franzke A, Rogler G, et al. Probiotic Escherichia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity. PLoS One 2007, 12:e1308.
20. Ghadimi D, Vrese MD, Heller KJ, Schrezenmeir J. Effect of natural commensal-origin DNA on toll-like receptor 9 (TLR9) signaling cascade, chemokine IL-8 expression, and barrier integrity of polarized intestinal epithelial cells. Inflamm Bowel Dis 2010, 16:410-427.
21. Fasano A. Zonulin and ist regulation of intestinal barrier function: the biological door to inflammation, autoimmunity, and cancer. Physiol Rev 2011, 91:151-175. 10.1152/physrev.00003.2008, 21248165.
22. Groschowitz KR, Hogan SP. Intestinal barrier function: molecular regulation and disease pathogenesis. J Allergy Clin Immunol 2009, 124:3-20. 10.1016/j.jaci.2009.05.038, 19560575.
23. Sonier B, Patrick C, Ajjikuttira P, Scott FW. Intestinal immune regulation as a potential diet-modifiable feature of gut inflammation and autoimmunity. Int Rev Immunol 2009, 28:414-445. 10.3109/08830180903208329, 19954357.
24. Commission of experts of the German Society of Sports Medicine and Prevention Guidelines for testing in sports medicine. Plane IV 2002, Germany: Commission of experts of the German Society of Sports Medicine and Prevention, Expertenkommission der Deutschen Gesellschaft für Sportmedizin und Prävention: Leitlinien zur Belastungsuntersuchung in der Sportmedizin. Ebene IV. Deutsche Gesellschaft für Sportmedizin und Prävention, März 2002 (German), Commission of experts of the German Society of Sports Medicine and Prevention.
25. Möller R, Tafeit E, Smolle KH, Pieber TR, Ipsiroglu O, Duesse M, Huemer C, Sudi K, Reibnegger G. Estimating percentage total body fat and determining subcutaneous adipose tissue distribution with a new non-invasive optical device Lipometer. Am J Hum Biol 2000, 12:221-230. 10.1002/(SICI)1520-6300(200003/04)12:2<221::AID-AJHB8>3.0.CO;2-2, 11534019.
26. Young DS. Implementation of SI units for clincal laboratory tables - style specifications and conversion tables. Ann Intern Med 1987, 106:114-129.
27. German Nutrition Society, Austrian Nutrition Society, Swiss Association of Nutrition: Reference values for nutrient intake, 3rd revision of the 1st issue.Umschau Braus Ltd:Frankfurt; 2008.Deutsche Gesellschaft für Ernährung (DGE), Österreichische Gesellschaft für Ernährung (ÖGE), Schweizerische Vereinigung für Ernährung (SVE): Referenzwerte für die Nährstoffzufuhr. 3. korrigierter Nachdruck der 1. Auflage. Frankfurt: Umschau Braus GmbH; 2008 (German).
28. Lewis SJ, Heaton KW. Stool form scale as a useful guide to intestinal transit time. Scand J Gastroenterol 1997, 32:920-924. 10.3109/00365529709011203, 9299672.
29. Pilz J, Meinekea I, Gleitera CH. Measurement of free and bound malondialdehyde in plasma by high-performance liquid chromatography as the 2,4-dinitrophenyl-hydrazine derivative. J Chromatogr B Biomed Sci Appl 2000, 742:315-325. 10.1016/S0378-4347(00)00174-2, 10901136.
30. Dill DB, Costill DL. Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. J Appl Physiol 1999, 73:1265-1272.
31. Baska RS, Moses FM, Graeber G, Kearney G. Gastrointestinal bleeding during an ultramarathon. Dig Dis Sci 1990, 35:276-279. 10.1007/BF01536777, 2302987.
32. Eichner ER. Gastrointestinal bleeding in athletes. Physician Sportsmed 1989, 17:128-140.
33. Oktedalen O, Lunde OC, Opstad PK, Aabakken L, Kvernebo K. Changes in the gastrointestinal mucosa after long-distance running. Scand J Gastroenterol 1992, 27:270-274. 10.3109/00365529209000073, 1589703.
34. Pals KL, Chang R-T, Ryan AJ, Gisolfi CV. Effect of running intensity on intestinal permeability. J Appl Physiol 1997, 82:571-576.
35. Fasano A. Intestinal zonulin: open sesame!. Gut 2001, 49:159-162. 10.1136/gut.49.2.159, 1728387, 11454785.
36. Sapone A, de Magistris L, Pietzak M, Clemente MG, Tripathi A, Cucca F, Lampis R, Kryszak D, Carteni M, Generoso M, Iafusco D, Prisco F, Laghi F, Riegler G, Carratu R, Counts D, Fasano A. Zonulin upregulation is associated with increased gut permeability in subjects with type 1 diabetes and their relatives. Diabetes 2006, 55:1443-1449. 10.2337/db05-1593, 16644703.
37. Wang W, Uzzau S, Goldblum SE, Fasano A. Human zonulin, a potential modulator of intestinal tight junctions. J Cell Sci 2000, 113:4435-4440.
38. El Asmar R, Panigrahi P, Bamford P, Berti I, Not R, Coppa GV, Catassi C, Fasano A. Host-dependent activation of the zonulin system is involved in the impairment of the gut barrier function following bacterial colonization. Gastroenterology 2002, 123:1607-1615. 10.1053/gast.2002.36578, 12404235.
39. Wells JM, Rossi O, Meijerink M, van Baarlen P. Epithelial crosstalk at the microbiota-mucosal interface. PNAS 2011, 108(Suppl 1):4607-4614. 3063605, 20826446.
40. Cario E, Gerken G, Podolsky DK. Toll-like receptor 2 controls mucosal inflammation by regulating epithelial barrier function. Gastroenerology 2004, 127:224-238.
41. Koning CJM, Jonkers DMAE, Stobberingh EE, Mulder L, Rombouts FM, Stockbruegger RW. The effect of a multispecies probiotic on the intestinal microbiota and bowel movements in healthy volunteers taking the antibiotic Amoxycillin. Am J Gastroenterol 2007, 102:1-12.
42. Jeukendrup AE, Vet-Joop K, Sturk A, Stegen JHJC, Senden J, Saris WHM, Wagenmakers AJM. Relationship between gastro-intestinal complaints and endotoxaemia, cytokine release and the acute-phase reaction during and after a long-distance triathlon in highly trained men. Clin Sci 2000, 98:47-55. 10.1042/CS19990258, 10600658.
43. Fujii T, Shimizu T, Takahashi K, Kishiro M, Ohkubo M, Akimoto K, Yamashiro Y. Fecal α1-antitrpysin concentrations as a measure of enteric protein loss after modified fontan operations. J Pediatr Gastroenterol Nutr 2003, 37:577-580. 10.1097/00005176-200311000-00014, 14581800.
44. Strygler B, Nicar MJ, Santangelo WC, Porter JL, Fordtran JS. Alpha1-antritrypsin excretion in stool in normal subjects and in patients with gastrointestinal disorders. Gastroenterology 1990, 99:1380-1387.
45. Levine RL, Stadtman ER. Oxidative modification of proteins during aging. Exp Gerontol 2001, 36:1495-1502. 10.1016/S0531-5565(01)00135-8, 11525872.
46. Pantke U, Volk T, Schmutzler M, Kox WJ, Sitte N, Grune T. Oxidized proteins as a marker during coronary heart surgery. Free Radic Biol Med 1999, 27:1080-1086. 10.1016/S0891-5849(99)00144-6, 10569640.
47. Lamprecht M, Oettl K, Schwaberger G, Hofmann P, Greilberger JF. Several indicators of oxidative stress, immunity, and illness improved in trained men consuming an encapsulated juice powder concentrate for 28 weeks. J Nutr 2007, 137:2737-2741.
48. Lamprecht M, Oettl K, Schwaberger G, Hofmann P, Greilberger JF. Protein modification responds to exercise intensity and antioxidant supplementation. Med Sci Sport Exerc 2009, 41:155-163.
49. Deepa D, Jayakumari B, Thomas SV. Lipid peroxidation in women with epilepsy. Ann Indian Acad Neurol 2008, 11:44-46. 10.4103/0972-2327.40225, 2781145, 19966978.
50. Lamprecht M, Hofmann P, Greilberger JF, Schwaberger G. Increased lipid peroxidation in trained men after 2 weeks of antioxidant supplementation. Int J Sport Nutr Exerc Metab 2009, 19:385-399.
51. Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malondialdehyde and related aldehydes. Free Radic Biol Med 1991, 11:81-128. 10.1016/0891-5849(91)90192-6, 1937131.
52. Febbraio MA, Pedersen BK. Muscle-derived interleukin-6: mechanisms for activation and possible biological roles. FASEB J 2002, 16:1335-1347. 10.1096/fj.01-0876rev, 12205025.
53. Petersen AM, Pedersen BK. The anti-inflammatory effect of exercise. J Appl Physiol 2005, 98:1154-1162. 10.1152/japplphysiol.00164.2004, 15772055.