Validated RP-UHPLC PDA and GC-MS methods for the analysis of psychoactive alkaloids in Sceletium tortuosum

South African Journal of Botany

Volumn 82, Issue , 2012, Pages 99-107

  Shikanga, E A ^a   Kamatou, G P P ^a   Chen, W ^a   Combrinck, S ^a   Viljoen, A M ^a  

^a TSHWANE UNIVERSITY OF TECHNOLOGY   (South Africa)

Author keywords

CNS; GC MS; ICH; Mesembrine; Method validation; PDA; PDE; Precision; RP UHPLC; S a; S b; S y x; Sceletium tortuosum; SIM

Indexed keywords

ALKALOID; BIOACCUMULATION; DICOTYLEDON; ELECTRONIC EQUIPMENT; GAS CHROMATOGRAPHY; MASS SPECTROMETRY; PHYTOCHEMISTRY; QUALITY CONTROL; QUANTITATIVE ANALYSIS; REGRESSION ANALYSIS;

SCELETIUM TORTUOSUM;

EID: 84867100499     PISSN: 02546299     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.sajb.2012.05.004     Document Type: Article

References (20)

1. Alali F.Q., Tahboub Y.R., Ibrahim E.S., Qandil A.M., Tawaha K., Burgess J.P., Sy A., Nakanishi Y., Kroll D.J., Oberlies N.H. Pyrrolizidine alkaloids from Echium glomeratum (Boraginaceae). Phytochemistry 2008, 69:2334-2346.
2. Gericke, N., Van Wyk, B.-E., 1999. Pharmaceutical compositions containing mesembrine and related compounds. US Patent Application number 09/194836. Patent no US 6288104B1.
3. Gericke N., Viljoen A.M. Sceletium-a review update. Journal of Ethnopharmacology 2008, 119:653-663.
4. Harvey A.L., Young L.C., Viljoen A.M., Gericke N.P. Pharmacological actions of South African medicinal and functional food plant Sceletium tortuosum and its principle alkaloids. Journal of Ethnopharmacology 2011, 137:1124-1129.
5. International Conference of Harmonisation ICH harmonised tripartite guideline: validation of analytical procedures: text and methodology Q2(R1) 2005, Geneva.
6. Kumar V. Potential medicinal plants for CNS disorders: an overview. Phytotherapy Research 2006, 20:1023-1035.
7. Li Y., Hu Z., He L. An approach to the development of binary chromatographic fingerprints of the total alkaloids from Caulophyllum robustum by high performance liquid chromatography/diode array detector and gas chromatography/mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 2007, 43:1667-1672.
8. Miller J.N., Miller J.C. Statistics and Chemometrics for Analytical Chemistry 2000, Prentice Hall, New York. 4th ed.
9. Minkoff K., Bergman E., Beck A.T., Beck R. Hopelessness, depression and attempted suicide. The American Journal of Psychiatry 1973, 130:455-459.
10. Patnala S., Kanfer I. Investigation of the phytochemical content of Sceletium tortuosum following preparation of "Kougoed" by fermentation. Journal of Ethnopharmacology 2009, 121:86-91.
11. Patnala S., Kanfer I. HPLC analysis of mesembrine-type alkaloids in Sceletium plant material used as an African traditional medicine. Journal of Pharmacy and Pharmaceutical Sciences 2010, 13:558-570.
12. Rajpur A.P., Sonanis M.C. Development and validation of a rapid RP-UHPLC PDA method for the determination of aspirin and dipyridamole in combined capsule formulation. International Journal of Pharmacy and Pharmaceutical Sciences 2011, 3:156-160.
13. Renger B., Vegh Z., Ferenczi-Fodor K. Validation of thin layer and high performance thin layer chromatographic methods. Journal of Chromatography. A 2011, 1218:2712-2721.
14. Shikanga E.A., Hamman J.H., Chen W., Combrinck S., Gericke N., Viljoen A.M. In vitro permeation of mesembrine-alkaloids from Sceletium tortuosum across porcine buccal, sublingual and intestinal mucosa. Planta Medica 2011, 77:1-9.
15. Shikanga E.A., Viljoen A., Combrinck S., Marston A. Isolation of Sceletium alkaloids by high-speed countercurrent chromatography. Phytochemistry Letters 2011, 4:190-193.
16. Smith M.T., Crouch N.R., Gericke N., Hirst M. Psychoactive constituents of the genus Sceletium N.E.Br. and other Mesembryanthemaceae: a review. Journal of Ethnopharmacology 1996, 50:119-130.
17. Smith M.T., Field C.R., Crouch N.R., Hirst M. The distribution of mesembrine alkaloids in selected taxa of the Mesembryanthemaceae and their modification in the Sceletium derived 'Kougoed'. Pharmaceutical Biology 1998, 36:173-179.
18. Van Wyk B.-E., Gericke N. People's Plants 2003, Briza Publications, Pretoria.
19. Lickerman A. The six reasons people attempt suicide Available at, Accessed on 13 Nov 2011. http://www.psychologytoday.com/blog/happiness-in-world/201004/.
20. Sceletium-Kougoed-a natural mood elevator Available at, Accessed 20 Jan 2009. http://www.Sceletium.org.