The preterm gut microbiota: Changes associated with necrotizing enterocolitis and infection

Acta Paediatrica, International Journal of Paediatrics

Volumn 101, Issue 11, 2012, Pages 1121-1127

  Stewart, C J ^a   Marrs, E C L ^b   Magorrian, S ^b   Nelson, A ^a   Lanyon, C ^a   Perry, J D ^b   Embleton, N D ^c   Cummings, S P ^a   Berrington, J E ^c  

^a NORTHUMBRIA UNIVERSITY   (United Kingdom)

^b FREEMAN HOSPITAL   (United Kingdom)

^c NEWCASTLE UPON TYNE HOSPITALS NHS FOUNDATION TRUST   (United Kingdom)

Author keywords

Culture; Molecular; Necrotizing enterocolitis; Preterm; Sepsis

Indexed keywords

ARTICLE; CLINICAL ARTICLE; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED STUDY; DENATURING GRADIENT GEL ELECTROPHORESIS; DISEASE ASSOCIATION; ENTEROCOCCUS FAECALIS; FECES ANALYSIS; FEMALE; HUMAN; INFANT; INTESTINE FLORA; LATE ONSET SEPSIS; MALE; NECROTIZING ENTEROCOLITIS; NONHUMAN; PREMATURITY; PRIORITY JOURNAL; SEPSIS;

CASE-CONTROL STUDIES; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA, BACTERIAL; ENTEROCOCCUS FAECALIS; ENTEROCOLITIS, NECROTIZING; FECES; FEMALE; HUMANS; INFANT, NEWBORN; INFANT, PREMATURE; INFANT, PREMATURE, DISEASES; MALE; MULTIVARIATE ANALYSIS; PRINCIPAL COMPONENT ANALYSIS; RNA, RIBOSOMAL, 16S; SEPSIS; STAPHYLOCOCCUS;

EID: 84867099916     PISSN: 08035253     EISSN: 16512227     Source Type: Journal    
DOI: 10.1111/j.1651-2227.2012.02801.x     Document Type: Article

References (29)

1. Macia L, Thorburn AN, Binge LC, Marino E, Rogers KE, Maslowski KM, et al., Microbial influences on epithelial integrity and immune function as a basis for inflammatory diseases. Immunol Rev 2012; 245: 164-76.
2. Morowitz MJ, Poroyko V, Caplan M, Alverdy J, Liu DC,. Redefining the role of intestinal microbes in the pathogenesis of necrotizing enterocolitis. Pediatrics 2010; 125: 777-85.
3. Westra-Meijer CM, Degener JE, Dzoljic-Danilovic G, Michel MF, Mettau JW,. Quantitative study of the aerobic and anaerobic faecal flora in neonatal necrotising enterocolitis. Arch Dis Child 1983; 58: 523-8.
4. Hoy C, Millar M, MacKay P, Godwin P, Langdale V, Levene M,. Quantitative changes in faecal microflora preceding necrotising enterocolitis in premature neonates. Arch Dis Child 1990; 65: 1057-9.
5. Frank DN, Zhu W, Sartor RB, Li E,. Investigating the biological and clinical significance of human dysbioses. Trends Microbiol 2011; 19: 427-34.
6. Berrington JE, Hearn RI, Bythell M, Wright C, Embleton ND,. Deaths in preterm infants: changing pathology over 2 decades. J Pediatr 2012; 160: 49-53.
7. Morowitz MJ, Denef VJ, Costello EK, Thomas BC, Poroyko V, Relman DA, et al., Strain-resolved community genomic analysis of gut microbial colonization in a premature infant. Proc Natl Acad Sci USA 2011; 108: 1128-34.
8. Koenig JE, Spor A, Scalfone N, Fricker AD, Stombaugh J, Knight R, et al., Succession of microbial consortia in the developing infant gut microbiome. Proc Natl Acad Sci USA 2010; 108: 4578-85.
9. Palmer C, Bik EM, DiGiulio DB, Relman DA, Brown PO,. Development of the human infant intestinal microbiota. PLoS Biol 2007; 5: 1556-73.
10. Trosvik P, Stenseth NC, Rudi K,. Convergent temporal dynamics of the human infant gut microbiota. ISME J 2010; 4: 151-8.
11. Stark P, Lee A,. The microbial ecology of the large bowel of breastfed and formula-fed infants during the first year of life. J Med Microbiol 1982; 15: 189-203.
12. Sakata H, Yoshioka H, Fujita K,. Development of the intestinal flora in very low birth weight infants compared to normal full-term newborns. Eur J Pediatr 1985; 144: 186-90.
13. Björkström MV, Hall L, Söderlund S, Håkansson EG, Håkansson S, Domellöf M,. Intestinal flora in very low-birth weight infants. Acta Paediatr 2009; 98: 1762-7.
14. Gewolb IH, Schwalbe RS, Taciak VL, Harrison TS, Panigrahi P,. Stool microflora in extremely low birthweight infants. Arch Dis Child Fetal Neonatal Ed 1999; 80: F167-73.
15. Millar MR, Linton CJ, Cade A, Glancy D, Hall M, Jalal H,. Application of 16S rRNA gene PCR to study bowel flora of preterm infants with and without necrotizing enterocolitis. J Clin Microbiol 1996; 34: 2506-10.
16. de la Cochetiere M-F, Piloquet H, des Robert C, Darmaun D, Galmiche J-P, Roze J-C,. Early intestinal bacterial colonization and necrotizing enterocolitis in premature infants: the putative role of Clostridium. Pediatr Res 2004; 56: 366-70.
17. Wang Y, Hoenig JD, Malin KJ, Qamar S, Petrof EO, Sun J, et al., 16S rRNA gene-based analysis of fecal microbiota from preterm infants with and without necrotizing enterocolitis. ISME J 2009; 3: 944-54.
18. Mshvildadze M, Neu J, Shuster J, Theriaque D, Li N, Mai V,. Intestinal microbial ecology in premature infants assessed with non-culture-based techniques. J Pediatr 2010; 156: 20-5.
19. Mai V, Young CM, Ukhanova M, Wang X, Sun Y, Casella G, et al., Fecal microbiota in premature infants prior to necrotizing enterocolitis. PLoS ONE 2011; 6: e20647.
20. Muyzer G, de Waal EC, Uitterlinden AG,. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 1993; 59: 695-700.
21. Baxter J, Cummings SP,. The current and future applications of microorganism in the bioremediation of cyanide contamination. Antonie Van Leeuwenhoek 2006; 90: 1-17.
22. Tourlomousis P, Kemsley EK, Ridgway KP, Toscano MJ, Humphrey TJ, Narbad A,. PCR-denaturing gradient gel electrophoresis of complex microbial communities: a two-step approach to address the effect of gel-to-gel variation and allow valid comparisons across a large dataset. Microb Ecol 2010; 59: 776-86.
23. Braak CJFT,. Canonical correspondence analysis: a new eigenvector technique for multivariate direct gradient analysis. Ecology 1986; 67: 1167-79.
24. Jiménez E, Marín ML, Martín R, Odriozola JM, Olivares M, Xaus J, et al., Is meconium from healthy newborns actually sterile? Res Microbiol 2008; 159: 187-93.
25. Are A, Aronsson L, Wang S, Greicus G, Lee KY, Gustaffson J, et al., Enterococcus faecalis from newborn babies regulate endogenous PPAR activity and IL-10 levels in colonic epithelial cells. PNAS 2008; 6: 1943-8.
26. Marques TM, Wall R, Ross RP, Fitzgerald GF, Ryan CA, Stanton C,. Programming infant gut microbiota: influence of dietary and environmental factors. Curr Opin Biotechnol 2010; 21: 149-56.
27. Embleton ND, Yates R,. Probiotics and other preventative strategies for necrotising enterocolitis. Semin Fetal Neonatal Med 2008; 13: 35-43.
28. Deshpande G, Rao S, Patole S,. Probiotics for prevention of necrotising enterocolitis in preterm neonates with very low birthweight: a systematic review of randomised controlled trials. Lancet 2007; 369: 1614-20.
29. Venkatesh MP, Abrams SA,. Oral lactoferrin for the prevention of sepsis and necrotizing enterocolitis in preterm infants. Cochrane Database Syst Rev 2010; 5: CD007137.