Preparing sample chambers for single-molecule FRET

Cold Spring Harbor Protocols

Volumn 7, Issue 10, 2012, Pages 1104-1108

  Joo, Chirlmin ^a   Ha, Taekjip ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN; BOVINE SERUM ALBUMIN; MACROGOL; DNA; PROTEIN; RNA;

ARTICLE; FLUORESCENCE RESONANCE ENERGY TRANSFER; IMMOBILIZATION; MICROSCOPY; CHEMISTRY; CONFORMATION; LABORATORY DIAGNOSIS; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY; PROTEIN CONFORMATION;

BOVINAE;

DNA; FLUORESCENCE RESONANCE ENERGY TRANSFER; MICROSCOPY; MOLECULAR BIOLOGY; NUCLEIC ACID CONFORMATION; PROTEIN CONFORMATION; PROTEINS; RNA; SPECIMEN HANDLING;

EID: 84867034988     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot071530     Document Type: Article

References (14)

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3. Joo C, Ha T. 2012a. Imaging and identifying impurities in single-molecule FRET studies. Cold Spring Harb Protoc doi: 10.1101/pdb.prot071548.
4. Joo C, Ha T. 2012b. Labeling DNA (or RNA) for single-molecule FRET. Cold Spring Harb Protoc doi: 10.1101/pdb.prot071027.
5. Joo C, Ha T. 2012c. Labeling proteins for single-molecule FRET. Cold Spring Harb Protoc doi: 10.1101/pdb.prot071035.
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7. Lee JY, Okumus B, Kim DS, Ha T. 2005. Extreme conformational diversity in human telomeric DNA. Proc Natl Acad Sci 102: 18938-18943.
8. McKinney SA, Declais AC, Lilley DM, Ha T. 2003. Structural dynamics of individual Holliday junctions. Nat Struct Biol 10: 93-97.
9. McKinney SA, Freeman AD, Lilley DM, Ha T. 2005. Observing spontaneous branch migration of Holliday junctions one step at a time. Proc Natl Acad Sci 102: 5715-5720.
10. Murphy MC, Rasnik I, Cheng W, Lohman TM, Ha T. 2004. Probing singlestranded DNA conformational flexibility using fluorescence spectroscopy. Biophys J 86: 2530-2537.
11. Myong S, Rasnik I, Joo C, Lohman TM, Ha T. 2005. Repetitive shuttling of a motor protein on DNA. Nature 437: 1321-1325.
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13. Rasnik I, Myong S, Cheng W, Lohman TM, Ha T. 2004. DNA-binding orientation and domain conformation of the E. coli Rep helicase monomer bound to a partial duplex junction: Single-molecule studies of fluorescently labeled enzymes. J Mol Biol 336: 395-408.
14. Tan E, Wilson TJ, Nahas MK, Clegg RM, Lilley DM, Ha T. 2003. A four-way junction accelerates hairpin ribozyme folding via a discrete intermediate. Proc Natl Acad Sci 100: 9308-9313.