A double in vivo biotinylation technique for objective assessment of aging and clearance of mouse erythrocytes in blood circulation

Transfusion Medicine and Hemotherapy

Volumn 39, Issue 5, 2012, Pages 335-341

  Saxena, Rajiv K ^a,b   Bhardwaj, Nitin ^a   Sachar, Sumedha ^a   Puri, Niti ^a   Khandelwal, Sanjay ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

^b SOUTH ASIAN UNIVERSITY   (India)

Author keywords

Double in vivo biotinylation; Erythrocyte aging; Erythrocyte removal; Mouse blood circulation

Indexed keywords

BIOTIN; CD147 ANTIGEN; CD47 ANTIGEN; PHOSPHATIDYLSERINE;

AGING; ANIMAL CELL; ANTIGEN EXPRESSION; AUTOFLUORESCENCE; BIOTINYLATION; CELL DENSITY; CELL DESTRUCTION; CIRCULATION; COHORT ANALYSIS; DOUBLE IN VIVO BIOTINYLATION; ERYTHROCYTE; FLOW CYTOMETRY; IN VIVO STUDY; MOUSE; NONHUMAN; REVIEW;

EID: 84866991552     PISSN: 16603796     EISSN: 16603818     Source Type: Journal    
DOI: 10.1159/000342524     Document Type: Review

References (20)

1. Waugh RE, Naria M, Jackson CW, Mueller TJ, Suzuki T, Dale GL: Rheologic properties of senescent erythrocytes: Loss of surface area and volumewith red blood cell age. Blood 1992;79:1351-1358.
2. Khandelwal S, Saxena RK: Assessment of survival of aging erythrocyte in circulation and attendant changes in size and CD147 expression by a novel two step biotinylation method. Exp Gerontol 2006; 41:855-861.
3. Khandelwal S, van Rooijen N, Saxena RK: Reduced expression of CD47 during murine red blood cell (RBC) senescence and its role in RBC clearance from the circulation. Transfusion 2007;47: 1725-1732.
4. Khandelwal S, Saxena RK: A role of phosphatidylserine externalization in clearance of erythrocytes exposed to stress but not in eliminating aging populations of erythrocyte in mice. Exp Gerontol 2008; 43:764-770.
5. Saxena RK, Khandelwal S: Aging and destruction of erythrocytes in mice. Curr Sci 2009;97:500-507.
6. Prankerd TA: The ageing of red cells. J Physiol 1958;143:325-331.
7. Rigas DA, Koler RD: Erythrocyte enzymes and reduced glutathione (GSH) in hemoglobin H disease: Relation to cell age and denaturation of hemoglobin H. J Lab Clin Med 1961;58:417-424.
8. Garby L, Hjelm M: Ultracentrifugal fractionation of human erythrocytes with respect to cell age. Blut 1963;9:284-291.
9. Piomelli S, Lurinsky G, Wasserman LR: The mechanism of red cell aging. Relationship between cell age and specific gravity evaluated by ultracentrifugation in a discontinuous density gradient. J Lab Clin Med 1967;69:659-674.
10. Borun ER, Figueroa WG, Perry SM: The distribution of Fe59 tagged human erythrocytes in centrifuged specimen as a function of cell age. J Clin Invest 1957;36:676-679.
11. Greenwalt TJ, Flory LL, Steane EA: Quantitative haemagglutination. III. Studies of separated populations of human red blood cells of different densities. Br J Haematol 1970;19:701-709.
12. O'Connell DJ, Caruso CJ, Sass MD: Separation of erythrocytes of different ages. Clin Chem 1965;11: 771-781
13. Prentice TC, Bishop C: Separation of rabbit cells by density methods and characteristics of separated layers. J Cell Physiol 1965;65:113-125.
14. Rennie CM, Thompson S, Parker AC, Maddy A: Human erythrocyte fractionation in 'Percoll' density gradients. Clin Chim Acta 1979;98:119-125.
15. Morrison M, Jackson CW, Mueller TJ, Huang T, Dockter ME, Walker WS, Singer JA, Edwards HH: Does red cell density correlate with cell age? Biomed Biochem Acta 1983;42:107.
16. Sachar S, Saxena RK: Cytotoxic effect of poly-dispersed single walled carbon nanotubes on erythrocytes in vitro and in vivo. PLoS One 2011;6:e22032.
17. Saxena RK, Chandrasekhar B: A novel non-phagocytic mechanism of erythrocyte destruction involving direct cell mediated lysis. Int J Hematol 2000; 71:227-237.
18. Saxena RK: Should erythrocyte destruction in vivo be through phagocytosis alone? J Biosci 2000;25:3-5.
19. Khandelwal S, Saxena RK: Age dependent increase in green autofluorescence of blood erythrocytes. J Biosci 2007;32:1139-1145.
20. Magnani M, Rossi L, Stocchi V, Cucchiarini L, Piacentini G, Fornaini G: Effect of age on some properties of mice erythrocytes. Mech Ageing Dev 1988;42:37-47.