CD14, CD16 and HLA-DR reliably identifies human monocytes and their subsets in the context of pathologically reduced HLA-DR expression by CD14hi/CD16neg monocytes: Expansion of CD14hi/CD16pos and contraction of CD14lo/CD16pos monocytes in acute liver failure

Cytometry Part A

Volumn 81 A, Issue 10, 2012, Pages 823-834

  Abeles, Robin Daniel ^a   McPhail, Mark J ^a,b   Sowter, David ^c   Antoniades, Charalambos G ^a,b   Vergis, Nikhil ^b   Vijay, Godhev K Manakkat ^a   Xystrakis, Emmanuel ^a   Khamri, Wafa ^b   Shawcross, Debbie L ^a   Ma, Yun ^a   Wendon, Julia A ^a   Vergani, Diego ^a  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

^c Becton Dickinson UK Ltd   (United Kingdom)

Author keywords

CD14; CD16; HLA DR; Human blood monocyte subsets; Liver failure

Indexed keywords

BIOLOGICAL MARKER; CD14 ANTIGEN; FC RECEPTOR; HLA DR ANTIGEN;

ACUTE LIVER FAILURE; ADOLESCENT; ADULT; AGED; ARTICLE; CASE CONTROL STUDY; CLASSIFICATION; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENETICS; HUMAN; IMMUNOLOGY; IMMUNOPHENOTYPING; LEUKOCYTE COUNT; MALE; MIDDLE AGED; MONOCYTE; PATHOLOGY;

ADOLESCENT; ADULT; AGED; ANTIGENS, CD14; BIOLOGICAL MARKERS; CASE-CONTROL STUDIES; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION; GENE EXPRESSION PROFILING; HLA-DR ANTIGENS; HUMANS; IMMUNOPHENOTYPING; LEUKOCYTE COUNT; LIVER FAILURE, ACUTE; MALE; MIDDLE AGED; MONOCYTES; RECEPTORS, IGG;

EID: 84866619398     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22104     Document Type: Article

References (52)

1. van Furth R, Cohn ZA. The origin and kinetics of mononuclear phagocytes. J Exp Med 1968; 128: 415-435.
2. Akiyama Y, Stevenson GW, Schlick E, Matsushima K, Miller PJ, Stevenson HC. Differential ability of human blood monocyte subsets to release various cytokines. J Leukoc Biol 1985; 37: 519-530.
3. Passlick B, Flieger D, Ziegler-Heitbrock HW. Identification and characterization of a novel monocyte subpopulation in human peripheral blood. Blood 1989; 74: 2527-2534.
4. Ziegler-Heitbrock HW, Strobel M, Kieper D, Fingerle G, Schlunck T, Petersmann I, Ellwart J, Blumenstein M, Haas JG. Differential expression of cytokines in human blood monocyte subpopulations. Blood 1992; 79: 503-511.
5. Grage-Griebenow E, Flad HD, Ernst M, Bzowska M, Skrzeczynska J, Pryjma J. Human MO subsets as defined by expression of CD64 and CD16 differ in phagocytic activity and generation of oxygen intermediates. Immunobiology 2000; 202: 42-50.
6. Almeida J, Bueno C, Alguero MC, Sanchez ML, de Santiago M, Escribano L, Diaz-Agustin B, Vaquero JM, Laso FJ, San Miguel JF, et al. Comparative analysis of the morphological, cytochemical, immunophenotypical, and functional characteristics of normal human peripheral blood lineage(-)/CD16(+)/HLA-DR(+)/CD14(-/lo) cells, CD14(+) monocytes, and CD16(-) dendritic cells. Clin Immunol 2001; 100: 325-338.
7. Ziegler-Heitbrock HW, Fingerle G, Strobel M, Schraut W, Stelter F, Schutt C, Passlick B, Pforte A. The novel subset of CD14+/CD16+ blood monocytes exhibits features of tissue macrophages. Eur J Immunol 1993; 23: 2053-2058.
8. Skrzeczynska-Moncznik J, Bzowska M, Loseke S, Grage-Griebenow E, Zembala M, Pryjma J. Peripheral blood CD14high CD16+ monocytes are main producers of IL-10. Scand J Immunol 2008; 67: 152-159.
9. Auffray C, Sieweke MH, Geissmann F. Blood monocytes: development, heterogeneity, and relationship with dendritic cells. Annu Rev Immunol 2009; 27: 669-692.
10. Grage-Griebenow E, Flad HD, Ernst M. Heterogeneity of human peripheral blood monocyte subsets. J Leukoc Biol 2001; 69: 11-20.
11. Cros J, Cagnard N, Woollard K, Patey N, Zhang SY, Senechal B, Puel A, Biswas SK, Moshous D, Picard C, et al. Human CD14dim monocytes patrol and sense nucleic acids and viruses via TLR7 and TLR8 receptors. Immunity 2010; 33: 375-386.
12. Weber C, Belge KU, von Hundelshausen P, Draude G, Steppich B, Mack M, Frankenberger M, Weber KS, Ziegler-Heitbrock HW. Differential chemokine receptor expression and function in human monocyte subpopulations. J Leukoc Biol 2000; 67: 699-704.
13. Ancuta P, Rao R, Moses A, Mehle A, Shaw SK, Luscinskas FW, Gabuzda D. Fractalkine preferentially mediates arrest and migration of CD16+ monocytes. J Exp Med 2003; 197: 1701-1707.
14. Fingerle G, Pforte A, Passlick B, Blumenstein M, Strobel M, Ziegler-Heitbrock HW. The novel subset of CD14+/CD16+ blood monocytes is expanded in sepsis patients. Blood 1993; 82: 3170-3176.
15. Fingerle-Rowson G, Auers J, Kreuzer E, Fraunberger P, Blumenstein M, Ziegler-Heitbrock LH. Expansion of CD14+CD16+ monocytes in critically ill cardiac surgery patients. Inflammation 1998; 22: 367-379.
16. Skrzeczynska J, Kobylarz K, Hartwich Z, Zembala M, Pryjma J. CD14+CD16+ monocytes in the course of sepsis in neonates and small children: Monitoring and functional studies. Scand J Immunol 2002; 55: 629-638.
17. Panasiuk A, Zak J, Kasprzycka E, Janicka K, Prokopowicz D. Blood platelet and monocyte activations and relation to stages of liver cirrhosis. World J Gastroenterol 2005; 11: 2754-2758.
18. Sayed D, Amin NF, Galal GM. Monocyte-platelet aggregates and platelet micro-particles in patients with post-hepatitic liver cirrhosis. Thromb Res 2010; 125: e228-e233.
19. Zimmermann HW, Seidler S, Nattermann J, Gassler N, Hellerbrand C, Zernecke A, Tischendorf JJ, Luedde T, Weiskirchen R, Trautwein C, et al. Functional contribution of elevated circulating and hepatic non-classical CD14CD16 monocytes to inflammation and human liver fibrosis. PLoS One 2010; 5: e11049.
20. Hepburn AL, Mason JC, Davies KA. Expression of Fcgamma and complement receptors on peripheral blood monocytes in systemic lupus erythematosus and rheumatoid arthritis. Rheumatology (Oxford) 2004; 43: 547-554.
21. Schlitt A, Heine GH, Blankenberg S, Espinola-Klein C, Dopheide JF, Bickel C, Lackner KJ, Iz M, Meyer J, Darius H, et al. CD14+CD16+ monocytes in coronary artery disease and their relationship to serum TNF-alpha levels. Thromb Haemost 2004; 92: 419-424.
22. Castano D, Garcia LF, Rojas M. Increased frequency and cell death of CD16+ monocytes with Mycobacterium tuberculosis infection. Tuberculosis (Edinb) 2011; 91: 348-360.
23. Vanham G, Edmonds K, Qing L, Hom D, Toossi Z, Jones B, Daley CL, Huebner B, Kestens L, Gigase P, et al. Generalized immune activation in pulmonary tuberculosis: Co-activation with HIV infection. Clin Exp Immunol 1996; 103: 30-34.
24. Ziegler-Heitbrock L, Ancuta P, Crowe S, Dalod M, Grau V, Hart DN, Leenen PJ, Liu YJ, MacPherson G, Randolph GJ, et al. Nomenclature of monocytes and dendritic cells in blood. Blood 2010; 116: e74-e80.
25. Tallone T, Turconi G, Soldati G, Pedrazzini G, Moccetti T, Vassalli G. Heterogeneity of human monocytes: An optimized four-color flow cytometry protocol for analysis of monocyte subsets. J Cardiovasc Transl Res 2011; 4: 211-219.
26. Autissier P, Soulas C, Burdo TH, Williams KC. Evaluation of a 12-color flow cytometry panel to study lymphocyte, monocyte, and dendritic cell subsets in humans. Cytometry A 2010; 77A: 410-419.
27. Heimbeck I, Hofer TP, Eder C, Wright AK, Frankenberger M, Marei A, Boghdadi G, Scherberich J, Ziegler-Heitbrock L. Standardized single-platform assay for human monocyte subpopulations: Lower CD14+CD16++ monocytes in females. Cytometry A 2010; 77A: 823-830.
28. Baehr RV, Volk HD, Reinke P, Falck P, Wolff H. An immune monitoring program for controlling immunosuppressive therapy. Transplant Proc 1989; 21: 1189-1191.
29. Volk HD, Thieme M, Heym S, Docke WD, Ruppe U, Tausch W, Manger D, Zuckermann S, Golosubow A, Nieter B, et al. Alterations in function and phenotype of monocytes from patients with septic disease-Predictive value and new therapeutic strategies. Behring Inst Mitt 1991: 208-215.
30. Volk HD, Reinke P, Krausch D, Zuckermann H, Asadullah K, Muller JM, Docke WD, Kox WJ. Monocyte deactivation-Rationale for a new therapeutic strategy in sepsis. Intensive Care Med 1996; 22: S474-S481.
31. Kox WJ, Volk T, Kox SN, Volk HD. Immunomodulatory therapies in sepsis. Intensive Care Med 2000; 26: S124-S128.
32. Giannoudis PV, Smith RM, Perry SL, Windsor AJ, Dickson RA, Bellamy MC. Immediate IL-10 expression following major orthopaedic trauma: Relationship to anti-inflammatory response and subsequent development of sepsis. Intensive Care Med 2000; 26: 1076-1081.
33. Klava A, Windsor A, Boylston AW, Reynolds JV, Ramsden CW, Guillou PJ. Monocyte activation after open and laparoscopic surgery. Br J Surg 1997; 84: 1152-1156.
34. Sachse C, Prigge M, Cramer G, Pallua N, Henkel E. Association between reduced human leukocyte antigen (HLA)-DR expression on blood monocytes and increased plasma level of interleukin-10 in patients with severe burns. Clin Chem Lab Med 1999; 37: 193-198.
35. Gardiner KR, Crockard AD, Halliday MI, Rowlands BJ. Class II major histocompatibility complex antigen expression on peripheral blood monocytes in patients with inflammatory bowel disease. Gut 1994; 35: 511-516.
36. Satoh A, Miura T, Satoh K, Masamune A, Yamagiwa T, Sakai Y, Shibuya K, Takeda K, Kaku M, Shimosegawa T. Human leukocyte antigen-DR expression on peripheral monocytes as a predictive marker of sepsis during acute pancreatitis. Pancreas 2002; 25: 245-250.
37. Berres ML, Schnyder B, Yagmur E, Inglis B, Stanzel S, Tischendorf JJ, Koch A, Winograd R, Trautwein C, Wasmuth HE. Longitudinal monocyte human leukocyte antigen-DR expression is a prognostic marker in critically ill patients with decompensated liver cirrhosis. Liver Int 2009; 29: 536-543.
38. Wasmuth HE, Kunz D, Yagmur E, Timmer-Stranghoner A, Vidacek D, Siewert E, Bach J, Geier A, Purucker EA, Gressner AM, et al. Patients with acute on chronic liver failure display sepsis-like immune paralysis. J Hepatol 2005; 42: 195-201.
39. Lin CY, Tsai IF, Ho YP, Huang CT, Lin YC, Lin CJ, Tseng SC, Lin WP, Chen WT, Sheen IS. Endotoxemia contributes to the immune paralysis in patients with cirrhosis. J Hepatol 2007; 46: 816-826.
40. Xing T, Li L, Cao H, Huang J. Altered immune function of monocytes in different stages of patients with acute on chronic liver failure. Clin Exp Immunol 2007; 147: 184-188.
41. Antoniades CG, Berry PA, Davies ET, Hussain M, Bernal W, Vergani D, Wendon J. Reduced monocyte HLA-DR expression: a novel biomarker of disease severity and outcome in acetaminophen-induced acute liver failure. Hepatology 2006; 44: 34-43.
42. Berry PA, Antoniades CG, Carey I, McPhail MJ, Hussain MJ, Davies ET, Wendon JA, Vergani D. Severity of the compensatory anti-inflammatory response determined by monocyte HLA-DR expression may assist outcome prediction in cirrhosis. Intensive Care Med 2011; 37: 453-460.
43. Poehlmann H, Schefold JC, Zuckermann-Becker H, Volk HD, Meisel C. Phenotype changes and impaired function of dendritic cell subsets in patients with sepsis: A prospective observational analysis. Crit Care 2009; 13: R119.
44. Monneret G, Lepape A, Voirin N, Bohe J, Venet F, Debard AL, Thizy H, Bienvenu J, Gueyffier F, Vanhems P. Persisting low monocyte human leukocyte antigen-DR expression predicts mortality in septic shock. Intensive Care Med 2006; 32: 1175-1183.
45. Lekkou A, Karakantza M, Mouzaki A, Kalfarentzos F, Gogos CA. Cytokine production and monocyte HLA-DR expression as predictors of outcome for patients with community-acquired severe infections. Clin Diagn Lab Immunol 2004; 11: 161-167.
46. O'Grady JG, Schalm SW, Williams R. Acute liver failure: Redefining the syndromes. Lancet 1993; 342: 273-275.
47. Levy MM, Fink MP, Marshall JC, Abraham E, Angus D, Cook D, Cohen J, Opal SM, Vincent JL, Ramsay G. 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference. Crit Care Med 2003; 31: 1250-1256.
48. Herzenberg LA, Tung J, Moore WA, Parks DR. Interpreting flow cytometry data: A guide for the perplexed. Nat Immunol 2006; 7: 681-685.
49. Parks DR, Roederer M, Moore WA. A new Logicle display method avoids deceptive effects of logarithmic scaling for low signals and compensated data. Cytometry A 2006; 69: 541-551.
50. Gordon S, Taylor PR. Monocyte and macrophage heterogeneity. Nat Rev Immunol 2005; 5: 953-964.
51. Asadullah K, Woiciechowsky C, Docke WD, Egerer K, Kox WJ, Vogel S, Sterry W, Volk HD. Very low monocytic HLA-DR expression indicates high risk of infection-Immunomonitoring for patients after neurosurgery and patients during high dose steroid therapy. Eur J Emerg Med 1995; 2: 184-190.
52. Docke WD, Hoflich C, Davis KA, Rottgers K, Meisel C, Kiefer P, Weber SU, Hedwig-Geissing M, Kreuzfelder E, Tschentscher P, et al. Monitoring temporary immunodepression by flow cytometric measurement of monocytic HLA-DR expression: A multicenter standardized study. Clin Chem 2005; 51: 2341-2347.