Deletion of a single helix from the transmembrane domain causes large changes in membrane insertion properties and secondary structure of the bacterial conjugation protein TrwB

Biochimica et Biophysica Acta - Biomembranes

Volumn 1818, Issue 12, 2012, Pages 3158-3166

  Vecino, Ana Julia ^a   Segura, Rosa De Lima ^a   De La Arada, Igor ^a   De La Cruz, Fernando ^b   Goñi, Félix M ^a   Arrondo, José L ^a   Alkorta, Itziar ^a  

^a UNIVERSITY OF THE BASQUE COUNTRY UPV EHU   (Spain)

^b UNIVERSITY OF CANTABRIA   (Spain)

Author keywords

Conjugative coupling protein; Infrared spectroscopy; Membrane protein; Membrane protein orientation; Membrane protein reconstitution; Transmembrane domain

Indexed keywords

ADENOSINE TRIPHOSPHATE; BACTERIAL CONJUGATION PROTEIN TRWB; DETERGENT; MEMBRANE PROTEIN; UNCLASSIFIED DRUG;

ALPHA HELIX; ARTICLE; BACTERIUM CONJUGATION; BINDING AFFINITY; CELL VACUOLE; CONTROLLED STUDY; CYTOSOL; ESCHERICHIA COLI; INFRARED SPECTROSCOPY; LIPID MEMBRANE; LIPID VESICLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; SUSPENSION; WILD TYPE;

CELL MEMBRANE; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; LIPID BILAYERS; LIPOSOMES; MEMBRANE LIPIDS; MUTATION; PROTEIN STRUCTURE, SECONDARY; SEQUENCE DELETION; TETRAHYDROFOLATE DEHYDROGENASE;

BACTERIA (MICROORGANISMS);

EID: 84866491678     PISSN: 00052736     EISSN: 18792642     Source Type: Journal    
DOI: 10.1016/j.bbamem.2012.08.015     Document Type: Article

References (32)

1. 1-ATPase-like molecular motor involved in DNA transport during bacterial conjugation Res. Microbiol. 157 2006 299 305
2. S. Bolland, M. Llosa, P. Avila, and F. de la Cruz General organization of the conjugal transfer genes of the IncW plasmid R388 and interactions between R388 and IncN and IncP plasmids J. Bacteriol. 172 1990 5795 5802
3. I. Hormaeche, I. Alkorta, F. Moro, J.M. Valpuesta, F.M. Goñi, and F. de la Cruz Purification and properties of TrwB, a hexameric, ATP-binding integral membrane protein essential for R388 plasmid conjugation J. Biol. Chem. 277 2002 46456 46462
4. I. Hormaeche, I. Iloro, J.L. Arrondo, F.M. Goñi, F. de la Cruz, and I. Alkorta Role of the transmembrane domain in the stability of TrwB, an integral protein involved in bacterial conjugation J. Biol. Chem. 279 2004 10955 10961
5. I. Hormaeche, R.L. Segura, A.J. Vecino, F.M. Goñi, F. de la Cruz, and I. Alkorta The transmembrane domain provides nucleotide binding specificity to the bacterial conjugation protein TrwB FEBS Lett. 580 2006 3075 3082
6. A.J. Vecino, R.L. Segura, B. Ugarte-Uribe, S. Águila, F. de la Cruz, F.M. Goñi, and I. Alkorta Reconstitution in liposome bilayers enhances nucleotide binding affinity and ATP-specificity of TrwB conjugative coupling protein Biochim. Biophys. Acta, Biomembr. 1798 2010 2160 2169
7. A.J. Vecino, I. de la Arada, R.L. Segura, F.M. Goñi, F. de la Cruz, J.L.R. Arrondo, and I. Alkorta Membrane insertion stabilizes the structure of TrwB the R388 conjugative plasmid coupling protein Biochim. Biophys. Acta, Biomembr. 1808 2011 1032 1039
8. M. Llosa, S. Bolland, and F. de la Cruz Genetic organization of the conjugal DNA processing region of the IncW plasmid R388 J. Mol. Biol. 235 1994 448 464
9. 1-ATPase interacts with a membrane protein component of a proton channel Nature 298 1982 867 869
10. E. Lanka, and B.M. Wilkins DNA processing reactions in bacterial conjugation Annu. Rev. Biochem. 64 1995 141 169
11. E. Cabezón, J.I. Sastre, and F. de la Cruz Genetic evidence of a coupling role for the TraG protein family in bacterial conjugation Mol. Gen. Genet. 254 1997 400 406
12. I. Tato, S. Zunzunegui, F. de la Cruz, and E. Cabezon TrwB, the coupling protein involved in DNA transport during bacterial conjugation, is a DNA-dependent ATPase Proc. Natl. Acad. Sci. U. S. A. 102 2005 8156 8161
13. I. Tato, I. Matilla, I. Arechaga, S. Zunzunegui, F. de la Cruz, and E. Cabezon The ATPase activity of the DNA transporter TrwB is modulated by protein TrwA: implications for a common assembly mechanism of DNA translocating motors J. Biol. Chem. 282 2007 25569 25576
14. N. Möker, P. Reihlen, R. Krämer, and S. Morbach Osmosensing properties of the histidine protein kinase MtrB from Corynebacterium glutamicum J. Biol. Chem. 282 2007 27666 27677
15. A. Stolov, A. Valverde, P. Ronald, and S. Burdman Purification of soluble and active RaxH, a transmembrane histidine protein kinase from Xanthomonas oryzae pv. oryzae required for AvrXa21 activity Mol. Plant. Pathol. 8 2007 93 101
16. R.B. Gennis Biomembranes: Molecular Structure and Function 1989 Springer-Verlag New York
17. M.J. Hope, M.B. Bally, G. Webb, and P.R. Cullis Production of large unilamellar vesicles by a rapid extrusion procedure. Characterization of size distribution, trapped volume and ability to maintain a membrane potential Biochim. Biophys. Acta 812 1985 55 65
18. A.J. Vecino. La reconstitución en liposomas de TrwB, un nanomotor que transporta DNA, revela la importancia del dominio transmembrana en la función de la proteína. (2009) PhD thesis. Universidad del País Vasco UPV/EHU, Spain.
19. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
20. D. Lichtenberg Characterization of the solubilization of lipid bilayers by surfactants Biochim. Biophys. Acta 821 1985 470 478
21. P.W. Holloway A simple procedure for removal of Triton X-100 from protein samples Anal. Biochem. 53 1973 304 308
22. C.S.F. Böttcher, C.M. Van Gent, and C. Fries A rapid and sensitive submicro phosphorus determination Anal. Chim. Acta 24 1961 203 204
23. S. Sanowar, and H. Le Moual Functional reconstitution of the Salmonella typhimurium PhoQ histidine kinase sensor in proteoliposomes Biochem. J. 390 2005 769 776
24. J.L. Arrondo, A. Muga, J. Castresana, and F.M. Goñi Quantitative studies of the structure of proteins in solution by Fourier-transform infrared spectroscopy Prog. Biophys. Mol. Biol. 59 1993 23 56
25. J.L. Arrondo, and F.M. Goñi Structure and dynamics of membrane proteins as studied by infrared spectroscopy Prog. Biophys. Mol. Biol. 72 1999 367 405
26. D.J. Moore, and R. Mendelsohn Adaptation to altered growth temperatures in Acholeplasma laidlawii B: Fourier transform infrared studies of acyl chain conformational order in live cells Biochemistry 33 1994 4080 4085
27. A. Blume, W. Hübner, and G. Messner Fourier transform infrared spectroscopy of 13CO-labeled phospholipids hydrogen bonding to carbonyl groups Biochemistry 27 1988 8239 8249
28. E. Goormaghtigh, V. Cabiaux, J. De Meutter, M. Rosseneu, and J.M. Ruysschaert Secondary structure of the particle associating domain of apolipoprotein B-100 in low-density lipoprotein by attenuated total reflection infrared spectroscopy Biochemistry 32 1993 6104 6110
29. L.K. Tamm, and S.A. Tatulian Infrared spectroscopy of proteins and peptides in lipid bilayers Q. Rev. Biophys. 30 1997 365 429
30. J. Staerk, J.P. Defour, C. Pecquet, E. Leroy, H. Antoine-Poirel, J. Brett, M. Itaya, S.O. Smith, W. Vainchenker, and S.N. Constantinescu Orientation-specific signalling by thrombopoietin receptor dimers EMBO J. 30 2011 4398 4413
31. N.M. Hooper Roles of proteolysis and lipid rafts in the processing of the amyloid precursor protein and prion protein Biochem. Soc. Trans. 33 2005 335 338
32. J.L. Rigaud, D. Lévy, G. Mosser, and O. Lambert Detergent removal by non-polar polystyrene beads Eur. Biophys. J. 27 1998 305 319