Generation of monoclonal antibody for 15-deoxy-?12,14-prostaglandin j2 and development of enzyme-linked immunosorbent assay for its quantification in culture medium of adipocytes

Applied Biochemistry and Biotechnology

Volumn 167, Issue 5, 2012, Pages 1107-1118

  Syeda, Pinky Karim ^a   Hossain, Mohammad Salim ^a   Chowdhury, Abu Asad ^a   Rahman, Mohammad Sharifur ^a   Nishimura, Kohji ^a   Jisaka, Mitsuo ^a   Nagaya, Tsutomu ^a   Shono, Fumiaki ^b   Yokota, Kazushige ^a  

^a SHIMANE UNIVERSITY   (Japan)

^b TOKUSHIMA BUNRI UNIVERSITY   (Japan)

Author keywords

14 Prostaglandin J2; 15 deoxy ?12; Cultured adipocytes; Enzyme linked immunosorbent assay; Maturation medium; Monoclonal antibody; PGD2; PGJ2

Indexed keywords

14-PROSTAGLANDIN J2; 15-DEOXY-?12; ADIPOCYTES; ENZYME LINKED IMMUNOSORBENT ASSAY; MATURATION MEDIUM; PGD2; PGJ2;

CELL CULTURE; CLONING; CURVE FITTING; TISSUE;

MONOCLONAL ANTIBODIES;

15 DEOXY DELTA12,14 PROSTAGLANDIN J2; 15 DEOXY DELTA12,14 PROSTAGLANDIN J2 MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY; UNCLASSIFIED DRUG;

ACCURACY; ADIPOCYTE; ANIMAL CELL; ANTIBODY DETECTION; CALIBRATION; CELL CULTURE; CONCENTRATION (PARAMETERS); CONFERENCE PAPER; CONTROLLED STUDY; CROSS REACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; MOUSE; NONHUMAN; QUANTITATIVE ANALYSIS; RELIABILITY; SENSITIVITY AND SPECIFICITY;

ADIPOCYTES; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; CELL LINE; CULTURE MEDIA; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMANS; IMMUNIZATION; MICE; MICE, INBRED BALB C; PROSTAGLANDIN D2;

EID: 84866430176     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-012-9616-9     Document Type: Conference Paper

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