The single biopsy approach is reliable for the measurement of muscle protein synthesis rates in vivo in older men

Journal of Applied Physiology

Volumn 113, Issue 6, 2012, Pages 896-902

  Burd, Nicholas A ^a   Pennings, Bart ^a   Groen, Bart B L ^a   Gijsen, Annemie P ^a   Senden, Joan M G ^a   Van Loon, Luc J C ^a  

^a MAASTRICHT UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Amino acid; Phenylalanine; Stable isotope

Indexed keywords

MUSCLE PROTEIN; PHENYLALANINE; PLASMA PROTEIN;

AGE; AGED; ARTICLE; BIOPSY; BIOSYNTHESIS; COMPARATIVE STUDY; HUMAN; INTRAVENOUS DRUG ADMINISTRATION; ISOTOPE LABELING; KINETICS; MALE; MASS FRAGMENTOGRAPHY; METABOLISM; METHODOLOGY; REPRODUCIBILITY; SKELETAL MUSCLE;

AGE FACTORS; AGED; BIOPSY; BLOOD PROTEINS; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; INFUSIONS, INTRAVENOUS; ISOTOPE LABELING; KINETICS; MALE; MUSCLE PROTEINS; MUSCLE, SKELETAL; PHENYLALANINE; REPRODUCIBILITY OF RESULTS;

EID: 84866418577     PISSN: 87507587     EISSN: 15221601     Source Type: Journal    
DOI: 10.1152/japplphysiol.00513.2012     Document Type: Article

References (16)

1. Balagopal P, Rooyackers OE, Adey DB, Ades PA, Nair KS. Effects of aging on in vivo synthesis of skeletal muscle myosin heavy-chain and sarcoplasmic protein in humans. Am J Physiol Endocrinol Metab 273: E790-E800, 1997.
2. Burd NA, Groen BB, Beelen M, Senden JM, Gijsen AP, van Loon LJ. The reliability of using the single-biopsy approach to assess basal muscle protein synthesis rates in vivo in humans. Metab Clin Exp 61: 931-936, 2012.
3. Burd NA, Wall BT, van Loon LJ. The curious case of anabolic resistance: old wives' tales or new fables? J Appl Physiol 112: 1233-1235, 2012.
4. Burd NA, West DW, Rerecich T, Prior T, Baker SK, Phillips SM. Validation of a single biopsy approach and bolus protein feeding to determine myofibrillar protein synthesis in stable isotope tracer studies in humans. Nutr Metab (Lond) 8: 15, 2011.
5. Calder AG, Anderson SE, Grant I, McNurlan MA, Garlick PJ. The determination of low d5-phenylalanine enrichment (0.002-009 atom percent excess), after conversion to phenylethylamine, in relation to protein turnover studies by gas chromatography/electron ionization mass spectrometry. Rapid Commun Mass Spectrom 6: 421-424, 1992.
6. Henderson GC, Dhatariya K, Ford GC, Klaus KA, Basu R, Rizza RA, Jensen MD, Khosla S, O'Brien P, Nair KS. Higher muscle protein synthesis in women than men across the lifespan, and failure of androgen administration to amend age-related decrements. FASEB J 23: 631-641, 2009.
7. Heys SD, McNurlan MA, Park KG, Milne E, Garlick PJ. Baseline measurements for stable isotope studies: an alternative to biopsy. Biomed Environ Mass Spectrom 19: 176-178, 1990.
8. Koopman R, van Loon LJ. Aging, exercise, and muscle protein metabolism. J Appl Physiol 106: 2040-2048, 2009.
9. Parise G, Mihic S, MacLennan D, Yarasheski KE, Tarnopolsky MA. Effects of acute creatine monohydrate supplementation on leucine kinetics and mixed-muscle protein synthesis. J Appl Physiol 91: 1041-1047, 2001.
10. Patterson BW, Zhang XJ, Chen Y, Klein S, Wolfe RR. Measurement of very low stable isotope enrichments by gas chromatography/mass spectrometry: application to measurement of muscle protein synthesis. Metab Clin Exper 46: 943-948, 1997.
11. Rennie MJ, Smith K, Watt PW. Measurement of human tissue protein synthesis: an optimal approach. Am J Physiol Endocrinol Metab 266: E298-E307, 1994.
12. Schwenk WF, Berg PJ, Beaufrere B, Miles JM, Haymond MW. Use of t-butyldimethylsilylation in the gas chromatographic/mass spectrometric analysis of physiologic compounds found in plasma using electron-impact ionization. Anal Biochem 141: 101-109, 1984.
13. Short KR, Vittone JL, Bigelow ML, Proctor DN, Nair KS. Age and aerobic exercise training effects on whole body and muscle protein metabolism. Am J Physiol Endocrinol Metab 286: E92-E101, 2004.
14. Smith GI, Villareal DT, Lambert CP, Reeds DN, Mohammed BS, Mittendorfer B. Timing of the initial muscle biopsy does not affect the measured muscle protein fractional synthesis rate during basal, postabsorptive conditions. J Appl Physiol 108: 363-368, 2010.
15. Verdijk LB, Snijders T, Beelen M, Savelberg HH, Meijer K, Kuipers H, Van Loon LJ. Characteristics of muscle fiber type are predictive of skeletal muscle mass and strength in elderly men. J Am Geriatr Soc 58: 2069-2075, 2010.
16. Volpi E, Sheffield-Moore M, Rasmussen BB, Wolfe RR. Basal muscle amino acid kinetics and protein synthesis in healthy young and older men. JAMA 286: 1206-1212, 2001.