Crystal structure and chitin oligosaccharide-binding mode of a 'loopful' family GH19 chitinase from rye, Secale cereale, seeds

FEBS Journal

Volumn 279, Issue 19, 2012, Pages 3639-3651

  Ohnuma, Takayuki ^a   Numata, Tomoyuki ^b   Osawa, Takuo ^b   Inanaga, Hideko ^b   Okazaki, Yoko ^a   Shinya, Shoko ^a   Kondo, Kaori ^a   Fukuda, Tatsuya ^a   Fukamizo, Tamo ^a  

^a KINKI UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

binding subsites; chitin oligosaccharide; crystal structure; family GH19 chitinase; NMR spectroscopy

Indexed keywords

AGLYCONE; ALANINE; CHITIN OLIGOSACCHARIDE; CHITINASE; N ACETYLGLUCOSAMINE; OLIGOSACCHARIDE; TRYPTOPHAN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; COMPLEX FORMATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SUBSTRATE COMPLEX; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTOPE LABELING; MOLECULAR WEIGHT; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PLANT SEED; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FAMILY; RYE; SEQUENCE ALIGNMENT; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BINDING SITES; CATALYSIS; CATALYTIC DOMAIN; CHITIN; CHITINASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRYSTALLOGRAPHY, X-RAY; HYDROLYSIS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SECALE CEREALE; SEEDS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

SECALE CEREALE;

EID: 84866383476     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2012.08723.x     Document Type: Article

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