Chitinase-catalyzed hydrolysis of 4-nitrophenyl penta-N-acetyl-β- chitopentaoside as determined by real-time ESIMS: The 4-nitrophenyl moiety of the substrate interacts with the enzyme binding site

Carbohydrate Research

Volumn 346, Issue 6, 2011, Pages 863-866

  Letzel, Thomas ^b   Sahmel Schneider, Ellen ^b   Skriver, Karen ^c   Ohnuma, Takayuki ^a   Fukamizo, Tamo ^a  

^a KINKI UNIVERSITY   (Japan)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

4 Nitrophenyl penta N acetyl chitopentaoside; Chitinase; Oligosaccharide hydrolysis; Real time ESIMS; Tryptophan residue

Indexed keywords

CHITINASE; CHITINASES; CLASS II; ENZYMATIC REACTION; ENZYME-BINDING SITES; MUTATED ENZYMES; REAL-TIME ESIMS; SIDE CHAINS; SUBSITES; SUBSTRATE BINDS; SUBSTRATE-BINDING; TRYPTOPHAN RESIDUE; TRYPTOPHAN RESIDUES; WILD-TYPE ENZYMES;

AMINO ACIDS; BINDING ENERGY; BINDING SITES; ENZYMES; HYDROLASES; HYDROLYSIS; OLIGOSACCHARIDES;

SUBSTRATES;

4 NITROPHENYL PENTA N ACETYL BETA CHITOPENTAOSIDE; ALANINE; CHITINASE; INDOLE; PHENYL GROUP; TRYPTOPHAN; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME BINDING; ENZYME MECHANISM; HYDROLYSIS; MUTATION; PRIORITY JOURNAL; SUBSTITUTION REACTION;

BINDING SITES; CATALYSIS; CHITINASE; HYDROLYSIS; MODELS, MOLECULAR; NITROPHENOLS; OLIGOSACCHARIDES; PROTEIN BINDING; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SUBSTRATE SPECIFICITY; TRYPTOPHAN;

HORDEUM;

EID: 79953297036     PISSN: 00086215     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.carres.2011.01.012     Document Type: Article

References (15)

1. Nanjo, F.; Sakai, K.; Usui, T. J. Biochem. 1988, 104, 255-258.
2. Hollis, T.; Honda, Y.; Fukamizo, T.; Marcotte, E.; Day, P. J.; Robertus, J. D. Arch. Biochem. Biophys. 1997, 344, 335-342.
3. Malet, C.; Planas, A. Biochemistry 1997, 36, 13838-13848.
4. Krokeide, I. M.; Synstad, B.; Gåseidnes, S.; Horn, S. J.; Eijsink, V. G.; Sørlie, M. Anal. Biochem. 2007, 363, 128-134.
5. Fukamizo, T.; Hayashi, K.; Tamoi, M.; Fujimura, Y.; Kurotaki, H.; Kulminskaya, A.; Kitaoka, M. Arch. Biochem. Biophys. 2008, 478, 187-194.
6. Sasaki, C.; Yokoyama, A.; Itoh, Y.; Hashimoto, M.; Watanabe, T.; Fukamizo, T. J. Biochem. 2002, 131, 557-564.
7. Sasaki, C.; Itoh, Y.; Takehara, H.; Kuhara, S.; Fukamizo, T. Plant Mol. Biol. 2003, 52, 43-52.
8. Mizuno, R.; Fukamizo, T.; Sugiyama, S.; Nishizawa, Y.; Kezuk, Y.; Nonaka, T.; Suzuki, K.; Watanabe, T. J. Biochem. 2008, 143, 487-495.
9. Taira, T.; Fujiwara, M.; Dennhart, N.; Hayashi, H.; Onaga, S.; Ohnuma, T.; Letzel, T.; Sakuda, S.; Fukamizo, T. Biochim. Biophys. Acta 2010, 1804, 668-675.
10. Fukamizo, T.; Miyake, R.; Tamura, A.; Ohnuma, T.; Skriver, K.; Pursiainen, N. V.; Juffer, A. H. Biochim. Biophys. Acta 2009, 1794, 1159-1167.
11. Dennhart, N.; Fukamizo, T.; Brzezinski, R.; Lacombe-Harvey, M. E.; Letzel, T. J. Biotechnol. 2008, 134, 253-260.
12. Dennhart, N.; Weigang, L. M.; Fujiwar, M.; Fukamizo, T.; Skriver, K.; Letzel, T. J. Biotechnol. 2009, 143, 274-283.
13. Pace, C. N.; Vajdos, F.; Fee, L.; Grimsley, G.; Gray, T. Protein Sci. 1995, 4, 2411-2423.
14. Honda, Y.; Fukamizo, T. Biochim. Biophys. Acta 1998, 1388, 53-65.
15. Dennhart, N.; Letzel, T. Anal. Bioanal. Chem. 2006, 386, 689-698.