Essential amino acid residues of BioY reveal that dimers are the functional S unit of the Rhodobacter capsulatus biotin transporter

Journal of Bacteriology

Volumn 194, Issue 17, 2012, Pages 4505-4512

  Kirsch, Franziska ^a   Frielingsdorf, Stefan ^a   Pohlmann, Anne ^a,b   Eitinger, Thomas ^a   Ziomkowska, Joanna ^a   Herrmann, Andreas ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

^b Institut für Virusdiagnostik   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN; BIOY PROTEIN; DIMER; ESSENTIAL AMINO ACID; MEMBRANE PROTEIN; UNCLASSIFIED DRUG;

ANISOTROPY; ARTICLE; BACTERIAL MUTATION; BINDING AFFINITY; ESCHERICHIA COLI; IN VIVO STUDY; MASS SPECTROMETRY; MOLECULAR INTERACTION; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN TRANSPORT; QUANTITATIVE ANALYSIS; RHODOBACTER CAPSULATUS; STOICHIOMETRY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BIOLOGICAL TRANSPORT; BIOTIN; ESCHERICHIA COLI; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RHODOBACTER CAPSULATUS; SYMPORTERS;

ESCHERICHIA COLI; PROKARYOTA; RHODOBACTER CAPSULATUS;

EID: 84866316663     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00683-12     Document Type: Article

References (25)

1. Bader AN, et al. 2011. Homo-FRET imaging as a tool to quantify protein and lipid clustering. Chemphyschem 12:475-483.
2. Bordoli L, et al. 2009. Protein structure homology modeling using SWISS-MODEL workspace. Nat. Protoc. 4:1-13.
3. Duurkens RH, Tol MB, Geertsma ER, Permentier HP, Slotboom DJ. 2007. Flavin binding to the high affinity riboflavin tra sporter RibU. J. Biol. Chem. 282:10380-10386.
4. Eitinger T, Rodionov DA, Grote M, Schneider E. 2011. Canonical and ECF-type ATP-binding cassette importers in prokaryo es: diversity in modular organization and cellular functions. FEMS Microbiol. Rev. 35:3-67.
5. Erkens GB, et al. 2011. The structural basis of modularity in ECF-type ABC transporters. Nat. Struct. Mol. Biol. 18:75-760.
6. Erkens GB, Slotboom DJ. 2010. Biochemical characterization of ThiT from Lactococcus lactis: a thiamin transporter with picomolar substrate binding affinity. Biochemistry 49:3203-3212.
7. Eudes A, et al. 2008. Identification of genes encoding the folate-and thiamine-binding membrane proteins in Firmicute . J. Bacteriol. 190:7591-7594
8. Finkenwirth F, et al. 2010. Subunit composition of an energy-couplingfactor-type biotin transporter analysed in living bacteria. Biochem. J. 431: 373-380.
9. Geertsma ER, Duurkens RH, Poolman B. 2005. Functional interactions between the subunits of the lactose transporter fro Streptococcus thermophilus. J. Mol. Biol. 350:102-111.
10. Glass JI, et al. 2006. Essential genes of a minimal bacterium. Proc. Natl. Acad. Sci. U. S. A. 103:425-430.
11. Hebbeln P, Rodionov DA, Alfandega A, Eitinger T. 2007. Biotin uptake in prokaryotes by solute transporters with an op ional ATP-binding cassette-containing module. Proc. Natl. Acad. Sci. U. S. A. 104:2909-2914.
12. Ketner G, Campbell A. 1974. A deletion mutation placing the galactokinase gene of Escherichia coli under control of t e biotin promoter. Proc. Natl. Acad. Sci. U. S. A. 71:2698-2702.
13. Neubauer O, et al. 2009. Two essential arginine residues in the T components of energy-coupling factor transporters. . Bacteriol. 191:6482-6488.
14. Neubauer O, Reiffler C, Behrendt L, Eitinger T. 2011. Interactions among the A and T units of an ECF-type biotin transporter analyzed by site-specific crosslinking. PLoS One 6:e29087. doi:10.1371/journal.pone.0029087.
15. Peterson GL. 1977. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 83:346-356.
16. Rodionov DA, et al. 2009. A novel class of modular transporters for vitamins in prokaryotes. J. Bacteriol. 191:42-51.
17. Rodionov DA, Hebbeln P, Gelfand MS, Eitinger T. 2006. Comparative and functional genomic analysis of prokaryotic nick l and cobalt uptake transporters: evidence for a novel group of ATP-binding cassette transporters. J. Bacteriol. 188:317-327.
18. Sahin-Toth M, Lawrence MC, Kaback HR. 1994. Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli. Proc. Natl. Acad. Sci U. S. A. 91:5421-5425.
19. Schauer K, Stolz J, Scherer S, Fuchs TM. 2009. Both thiamine uptake and biosynthesis of thiamine precursors are required for intracellular replication of Listeria monocytogenes. J. Bacteriol. 191:2218-2227.
20. Siche S, Neubauer O, Hebbeln P, Eitinger T. 2010. A bipartite S unit of an ECF-type cobalt transporter. Res. Microbio . 161:824-829.
21. Steiner-Mordoch S, et al. 2008. Parallel topology of genetically fused EmrE homodimers. EMBO J. 27:17-26.
22. ter Beek J, Duurkens RH, Erkens GB, Slotboom DJ. 2011. Quaternary structure and functional unit of energy coupling factor (ECF)-type transporters. J. Biol. Chem. 286:5471-5475.
23. Thanassi JA, Hartman-Neumann SL, Dougherty TJ, Dougherty BA, Pucci MJ. 2002. Identification of 113 conserved essentia genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res. 30:3152-3162.
24. Walker JR, Altman E. 2005. Biotinylation facilitates the uptake of large peptides by Escherichia coli and other gram-negative bacteria. Appl. Environ. Microbiol. 71:1850-1855.
25. Zhang P, Wang J, Shi Y. 2010. Structure and mechanism of the S component of a bacterial ECF transporter. Nature 468:717-720.