Identification of in vivo and in vitro metabolites of triptolide by liquid chromatography-tandem mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis

Volumn 70, Issue , 2012, Pages 624-630

  Peng, Zhi hong ^a   Wang, Jun jun ^a   Du, Peng ^a   Chen, Yong ^a  

^a HUBEI UNIVERSITY   (China)

Author keywords

LC MSn; Metabolite; Rat intestinal flora; Rat liver microsome; Triptolide

Indexed keywords

ACETONITRILE; DRUG METABOLITE; GLUCURONIDE; GLUTATHIONE; SULFATE; TRIPTOLIDE; WATER;

ANALYTICAL EQUIPMENT; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DRUG CONJUGATION; DRUG HYDROLYSIS; DRUG HYDROXYLATION; DRUG METABOLISM; ELECTROSPRAY MASS SPECTROMETRY; FEMALE; IN VITRO STUDY; IN VIVO STUDY; INTESTINE FLORA; ION TRAP MASS SPECTROMETRY; MALE; MASS SPECTROMETER; NONHUMAN; PRIORITY JOURNAL; RAT; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SENSITIVITY AND SPECIFICITY; SINGLE DRUG DOSE; TANDEM MASS SPECTROMETRY;

ACETONITRILES; ADMINISTRATION, ORAL; ANIMALS; BACTERIA; BIOTRANSFORMATION; CHROMATOGRAPHY, LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; DITERPENES; EPOXY COMPOUNDS; FEMALE; GLUCURONIDES; GLUTATHIONE; HYDROLYSIS; HYDROXYLATION; INTESTINES; MALE; MICROSOMES, LIVER; MOLECULAR STRUCTURE; PHENANTHRENES; RATS; RATS, WISTAR; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SULFATES; TANDEM MASS SPECTROMETRY; WATER;

EID: 84866261720     PISSN: 07317085     EISSN: 1873264X     Source Type: Journal    
DOI: 10.1016/j.jpba.2012.06.026     Document Type: Article

References (18)

1. Liu J., Wu Q.L., Feng Y.H., Wang Y.F., Li X.Y., Zuo J.P. Triptolide suppresses CD80 and CD86 expressions and IL-12 production in THP-1 cells. Acta Pharmacol. Sin. 2005, 26:223-227.
2. Yang S., Chen J., Guo Z., Xu X.M., Wang L., Pei X.F., Yang J., Underhill C.B., Zhang L. Triptolide inhibits the growth and metastasis of solid tumors. Mol. Cancer Ther. 2003, 2:65-72.
3. Panichakul T., Wanun T., Reutrakul V., Sirisinha S. Synergistic cytotoxicity and apoptosis induced in human cholangiocarcinoma cell lines by a combined treatment with tumor necrosis factor-alpha (TNF-alpha) and triptolide. Asian Pac. J. Allergy Immunol. 2002, 20:167-173.
4. Lou Y.J., Jie J., Wang Y.G. Triptolide inhibits transcription factor NF-kappa B and induces apoptosis of multiple myeloma cells. Leuk. Res. 2005, 29:99-105.
5. Matlin S.A., Belenguer A., Stacey V.E., Qian S.Z., Xu Y., Zhang J.W., Sanders J.K., Amor S.R., Pearce C.M. Male antifertility compounds from Tripterygium wilfordii Hook f. Contraception 1993, 47:387-400.
6. Tao X., Cush J.J., Garret M., Lipsky P.E. A phase I study of ethyl acetate extract of the Chinese antirheumatic herb Tripterygium wilfordii hook F in rheumatoid arthritis. J. Rheumatol. 2001, 28:2160-2167.
7. Sun X., Zhang S.M., Tian C.H., Yang L., Wang L.M., Li S.L. Safety of Tripterygium Wilfordii, Chinese. J. New Drugs 2001, 10:539-543.
8. Mei Z., Li X., Wu Q., Hu S., Yang X. The research on the anti-inflammatory activity and hepatotoxicity of triptolide-loaded solid lipid nanoparticle. Pharmacol. Res. 2005, 51:345-351.
9. Yang F., Zhuo L., Ananda S., Sun T., Li S., Liu L. Role of reactive oxygen species in triptolide-induced apoptosis of renal tubular cells and renal injury in rats. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 2011, 31:335-341.
10. Huang X.W., Xu J.H., Chen Y.Z. Tissue distribution of triptolide in rats. Chin. J. Clin. Pharmacol. Ther. 2008, 13:764-767.
11. Shao F., Wang G., Xie H., Zhu X., Sun J., A J. Pharmacokinetic study of triptolide, a constituent of immunosuppressive Chinese herb medicine, in rats. Biol. Pharm. Bull. 2007, 30:702-707.
12. Li W., Liu Y., He Y.Q., Zhang J.W., Gao Y., Ge G.B., Liu H.X., Huo H., Liu H.T., Wang L.M., Sun J., Wang Q., Yang L. Characterization of triptolide hydroxylation by cytochrome P450 in human and rat liver microsomes. Xenobiotica 2008, 38:1551-1565.
13. Liu L., Jiang Z., Liu J., Huang X., Wang T., Zhang Y., Zhou Z., Guo J., Yang L., Chen Y., Zhang L. Sex differences in subacute toxicity and hepatic microsomal metabolism of triptolide in rats. Toxicology 2010, 271:57-63.
14. Hattori M., Shu Y.Z., Shimizu M., Hayashi T., Morita N., Kobashi K., Xu G.J., Namba T. Metabolism of paeoniflorin and related compounds by human intestinal bacteria. Chem. Pharm. Bull. 1985, 33:3838-3846.
15. Gu J.K., Zhong D.F., Chen X.Y. Analysis of O-glucuronide conjugates in urine by electrospray ion trap mass spectrometry, Fresenius. J. Anal. Chem. 1999, 365:553-558.
16. Chen X., Zhong D., Jiang H., Gu J. Characterization of some glucuronide conjugates by electrospray ion trap mass spectrometry. Acta Pharmacol. Sin. 1998, 33:849-854.
17. Lu X.Y., Ma P.C. Fine stereo-structure study on Triperygium Wilford Hook F diterpene compounds. Nat. Prod. Res. Dev. 1995, 10:1-5.
18. Zhang F., Li Y.C. Progress in structure modification of triptolide. Acta Pharmacol. Sin. 2004, 39:857-864.