Discovery of a trans-dichloroethene-respiring Dehalogenimonas species in the 1,1,2,2-tetrachloroethane-dechlorinating WBC-2 consortium

Applied and Environmental Microbiology

Volumn 78, Issue 15, 2012, Pages 5280-5287

  Manchester, Marie J ^a   Hug, Laura A ^a   Zarek, Matt ^a,b   Zila, Anna ^a   Edwards, Elizabeth A ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

16S RRNA GENE; DEHALOCOCCOIDES; ENRICHMENT CULTURE; QUANTITATIVE PCR; TETRACHLOROETHANE; VINYL CHLORIDES;

CHLORINE COMPOUNDS; DNA SEQUENCES; ETHYLENE; GENES; HYDROGENOLYSIS; HYDROLYSIS; POLYMERASE CHAIN REACTION; REACTION KINETICS; RNA;

DECHLORINATION;

1,1,2,2-TETRACHLOROETHANE; CHLORINATED HYDROCARBON; DRUG DERIVATIVE; ETHANE; PRIMER DNA; RNA 16S; TETRACHLOROETHANE;

BACTERIOLOGY; BACTERIUM; DECHLORINATION; ETHYLENE; MICROBIAL ACTIVITY; MICROBIAL COMMUNITY; ORGANOHALOGEN; POLYMERASE CHAIN REACTION;

ARTICLE; CHLOROFLEXI; CULTURE MEDIUM; GAS CHROMATOGRAPHY; GENE LIBRARY; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; SPECIES DIFFERENCE;

CHLOROFLEXI; CHROMATOGRAPHY, GAS; CULTURE MEDIA; DNA PRIMERS; ETHANE; GENE LIBRARY; HYDROCARBONS, CHLORINATED; POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SPECIES SPECIFICITY;

DEHALOBACTER; DEHALOCOCCOIDES;

EID: 84866159880     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00384-12     Document Type: Article

References (31)

1. ATSDR. 2011. CERCLA priority list of hazardous substances. http://www.atsdr.cdc.gov/SPL/.
2. Aulenta F, et al. 2007. Field study of in situ anaerobic bioremediation of a chlorinated solvent source zone. Ind. Eng. Chem. Res. 46:6812-6819.
3. Aulenta F, Potalivo M, Majone M, Papini MP, Tandoi V. 2006. Anaerobic bioremediation of groundwater containing a mixture of 1,1,2,2-tetrachloroethane and chloroethenes. Biodegradation 17:193-206.
4. Chen C, Puhakka JA, Ferguson JF. 1996. Transformations of 1,1,2,2-tetrachloroethane under methanogenic conditions. Environ. Sci. Technol. 30:542-547.
5. Cheng D, Chow WL, He JZ. 2010. A Dehalococcoides-containing coculture that dechlorinates tetrachloroethene to trans-1,2-dichloroethene. ISME J. 4:88 -97.
6. Cupples AM, Spormann AM, McCarty PL. 2004. Vinyl chloride and cis-dichloroethene dechlorination kinetics and microorganism growth under substrate limiting conditions. Environ. Sci. Technol. 38:1102-1107.
7. Duhamel M, Edwards EA. 2006. Microbial composition of chlorinated ethene-degrading cultures dominated by Dehalococcoides. FEMS Microbiol. Ecol. 58:538-549.
8. Duhamel M, Mo K, Edwards EA. 2004. Characterization of a highly enriched Dehalococcoides-containing culture that grows on vinyl chloride and trichloroethene. Appl. Environ. Microbiol. 70:5538 -5545.
9. Edgar RC. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32:1792-1797.
10. Edwards EA, Grbic-Galic D. 1994. Anaerobic degradation of toluene and o-xylene by a methanogenic consortium. Appl. Environ. Microbiol. 60: 313-322.
11. Griffin BM, Tiedje JM, Loffler FE. 2004. Anaerobic microbial reductive dechlorination of tetrachloroethene to predominately trans-1,2-dichloroethene. Environ. Sci. Technol. 38:4300-4303.
12. Grostern A, Edwards EA. 2006. Growth of Dehalobacter and Dehalococcoides spp. during degradation of chlorinated ethanes. Appl. Environ. Microbiol. 72:428-436.
13. He J, Sung Y, Krajmalnik-Brown R, Ritalahti KM, Loffler FE. 2005. Isolation and characterization of Dehalococcoidessp. strain FL2, a trichloroethene (TCE)- and 1,2-dichloroethene-respiring anaerobe. Environ. Microbiol. 7:1442-1450.
14. Hendrickson ER, et al. 2002. Molecular analysis of Dehalococcoides 16S ribosomal DNA from chloroethene-contaminatedsites throughout north America and Europe. Appl. Environ. Microbiol. 68:485- 495.
15. Huber T, Faulkner G, Hugenholtz P. 2004. Bellerophon: a program to detect chimeric sequences in multiple sequence alignments. Bioinformatics 20:2317-2319.
16. Jones E, Voytek M, Lorah M, Kirshtein J. 2006. Characterization of a microbial consortium capable of rapid and simultaneous dechlorination of 1, 1, 2, 2-tetrachloroethane and chlorinated ethane and ethene intermediates. Bioremed. J. 10:153-168.
17. Löffler FE, et al. 2012. Dehalococcoides mccartyi gen. nov., sp. nov., obligate organohalide-respiring anaerobic bacteria, relevant to halogen cycling and bioremediation, belong to a novel bacterial class, Dehalococcoidetes classis nov., within the phylum Chloroflexi. Int. J. Syst. Evol. Microbiol. doi:10.1099/ijs.0.034926-0.
18. Lorah MM, Majcher EH, Jones EJ, Voytek MA. 2007. Microbial consortia development and microcosm and column experiments for enhanced bioremediation of chlorinated volatile organic compounds, West Branch Canal Creek wetland area, Aberdeen Proving Ground, Maryland. U.S. Geological Survey Scientific Investigations Report 2007-5165. US Department of the Interior,Washington, DC.
19. Lorah MM, Olsen LD. 1999. Degradation of 1,1,2,2-tetrachloroethane in a freshwater tidal wetland: field and laboratory evidence. Environ. Sci. Technol. 33:227-234.
20. Lorah MM, Olsen LD. 1999. Natural attenuation of chlorinated volatile organic compounds in a freshwater tidal wetland: field evidence of anaerobic biodegradation. Water Resour. Res. 35:3811-3827.
21. Lorah MM, Vogler ET, Dennis P, Graves D, Gallegos J. 2008. Labora-tory and in situ tests for bioremediation of hexahydro-1,3,5-trinitro- 1,3,5-triazine (RDX) by the dechlorinating consortium WBC-2, in remediation of chlorinated and recalcitrant compounds. Proceedings of the Sixth International Battelle Conference, Monterey, CA.
22. Moe WM, Yan J, Nobre MF, da Costa MS, Rainey FA. 2009. Dehalogenimonas lykanthroporepellens gen. nov., sp. nov., a reductively dehalogenating bacterium isolated from chlorinated solvent-contaminated groundwater. Int. J. Syst. Evol. Microbiol. 59:2692-2697.
23. Pfaffl MW. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e45. doi:10.1093/nar/29.9.e45.
24. Ritalahti KM, et al. 2010. Comparing on-site to off-site biomass collection for Dehalococcoides biomarker gene quantification to predict in situ chlorinated ethene detoxification potential. Environ. Sci. Technol. 44:5127-5133.
25. Rossetti S, Aulenta F, Majone M, Crocetti G, Tandoi V. 2008. Structure analysis and performance of a microbial community from a contaminated aquifer involved in the complete reductive dechlorination of 1,1,2,2- tetrachloroethane to ethene. Biotechnol. Bioeng. 100:240-249.
26. Sung Y, et al. 2003. Characterization of two tetrachloroethenereducing, acetate-oxidizing anaerobic bacteria and their description as Desulfuromonas michiganensis sp. nov. Appl. Environ. Microbiol. 69:2964-2974.
27. Suyama A, et al. 2001. Isolation and characterization of Desulfitobacterium sp. strain Y51 capable of efficient dehalogenation of tetrachloroethene and polychloroethanes. Biosci. Biotechnol. Biochem. 65:1474-1481.
28. US Department of Health and Human Services. 2008. Toxicological profile for 1,1,2,2-tetrachloroethane. US Department of Health and Human Services, Agency for Toxic Substances and Disease Registry, Atlanta, GA.
29. US Environmental Protection Agency. 2010. Toxicological review of 1,1,2,2-tetrachloroethane. US Environmental Protection Agency, Washington, DC.
30. van Eekert MHA, Stams AJM, Field JA, Schraa G. 1999. Gratuitous dechlorination of chloroethanes by methanogenic granular sludge. Appl. Microbiol. Biotechnol. 51:46-52.
31. Yan J, Rash BA, Rainey FA, Moe WM. 2009. Detection and quantification of Dehalogenimonas and "Dehalococcoides" populations via PCR based protocols targeting 16S rRNA genes. Appl. Environ. Microbiol. 75:7560-7564.