Rapid identification of heterozygous and homozygous Hemoglobin Constant Spring by SYTO9 with a high resolution melting analysis

Clinical Laboratory

Volumn 58, Issue 7-8, 2012, Pages 829-833

  Pornprasert, Sakorn ^a   Panyasai, Sitthichai ^b   Waneesorn, Jarurin ^c   Kongthai, Kanyakan ^d   Singboottra, Panthong ^a  

^a CHIANG MAI UNIVERSITY   (Thailand)

^b Naresuan Phayao University   (Thailand)

^c Regional Medical Sciences Center   (Thailand)

^d Health Promoting Hospital Chiang Mai   (Thailand)

Author keywords

Amplification refractory mutation system; Hemoglobin Constant Spring; High resolution melting analysis; SYTO9

Indexed keywords

GREEN FLUORESCENT PROTEIN; HEMOGLOBIN; HEMOGLOBIN CONSTANT SPRING; UNCLASSIFIED DRUG;

ALLELE; AMPLIFICATION REFRACTORY MUTATION SYSTEM POLYMERASE CHAIN REACTION; ARTICLE; BLOOD SAMPLING; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; HETEROZYGOSITY; HETEROZYGOTE; HIGH RESOLUTION MELTING ANALYSIS; HOMOZYGOTE; HUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; WILD TYPE;

BASE SEQUENCE; DNA PRIMERS; HEMOGLOBINS; HETEROZYGOTE; HOMOZYGOTE; HUMANS; POLYMERASE CHAIN REACTION;

EID: 84866135069     PISSN: 14336510     EISSN: None     Source Type: Journal    
DOI: 10.7754/Clin.Lab.2011.110903     Document Type: Article

References (17)

1. Fucharoen S, Winichagoon P. Hemoglobinopathies in Southeast Asia. Hemoglobin 1987;11:65-88.
2. Laig M, Pape M, Hundrieser J, et al. The distribution of the Hb constant spring gene in Southeast Asian populations. Hum Genet 1990;84:188-90.
3. Fucharoen S, Winichagoon P, Pootrakul P, Piankijagum A, Wasi P. Difference between two types of Hb H disease, alpha-thalassemia 1/alpha-thalassemia 2 and alpha-thalassemia 1/Hb constant spring. Birth Defects Orig Artic Ser 1987;23:309-15.
4. Tangvarasittichai O, Jeenapongsa R, Sitthiworanan C, Sanguansermsri T. Laboratory investigations of Hb Constant Spring. Clin Lab Haematol 2005;27:47-9.
5. Liao C, Zhou JY, Xie XM, Li J, Li R, Li DZ. Detection of Hb Constant Spring by a capillary electrophoresis method. Hemoglobin 2010;34:175-8.
6. Fucharoen S, Sanchaisuriya K, Fucharoen G, Panyasai S, Devenish R, Luy L. Interaction of haemoglobin E and several forms of alpha-thalassemia in Cambodian families. Haematologica 2003;88:1092-8.
7. Winichagoon P, Svasti S, Munkongdee T, et al. Rapid diagnosis of thalassemias and other hemoglobinopathies by capillary electrophoresis system. Transi Res 2008;152:178-84.
8. Ne W, Harano K, Harano T, et al. Hb Constant Spring [alpha 142, Term - >Gln (TAA>CAA in alpha2)] in the alpha-thalassemia of anemic patients in Myanmar. Hemoglobin 2008;32:454-61.
9. Singsanan S, Fuchareon G, Savongsy O, Sanchaisuriya K, Fuchareon S. Molecular characterization and origins of Hb Constant Spring and Hb Pakse in Southeast Asian populations. Ann Hematol 2007;86:665-9.
10. Chamras U, Sukunthamala K, Pornprasert S. SYTO9 and SYBR GREEN1 with high resolution melting analysis for molecular confirmatory testing of the common Southeast Asian beta(0)-thalassemia mutations. Hemoglobin 2009;33:539-45.
11. Pornprasert S, Phusua A, Suanta S, Saetung R, Sanguansermsri T. Detection of alpha-thalassemia-1 Southeast Asian type using real-time gap-PCR with SYBR Green1 and high resolution melting analysis. Eur J Haematol 2008;80:510-4.
12. Pornprasert S, Sukunthamala K. SYTO9 and SYBR GREEN1 with a high-resolution melting analysis for prenatal diagnosis of beta-thalassemia/ hemoglobin-E. Eur J Haematol 2010;85:424-9.
13. Prathomtanapong P, Pornprasert S, Phusua A, Suanta S, Saetung R, Sanguansermsri T. Detection and identification of beta-thalassemia 3.5 kb deletion by SYBR Green1 and high resolution melting analysis. Eur J Haematol 2009;82:159-60.
14. Li R, Liao C, Li D, Li J. High-resolution melting analysis of the three common nondeletional alpha-thalassemia mutations in the Chinese population: Hbs Constant Spring, Quong Sze and West-mead. Hemoglobin 2010;34:587-93.
15. Panyasai S, Sukunthamala K, Pornprasert S. Molecular confirmatory testing of hemoglobin Constant Spring by real-time polymerase chain reaction SYBR Green1 with high-resolution melting analysis. Eur J Haematol 2010;84:550-2.
16. Vossen RH, Aten E, Roos A, den Dunnen JT. High-resolution melting analysis (HRMA): more than just sequence variant screening. Hum Mutat 2009;30:860-6.
17. Monis PT, Goglio S, Saint CP. Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis. Anal Biochem 2005;340:24-34.