Hypoxic culture of human pluripotent stem cell lines is permissible using mouse embryonic fibroblasts

Regenerative Medicine

Volumn 7, Issue 5, 2012, Pages 675-683

  Badger, Jennifer L ^a,b   Byrne, Meg L ^a,b   Veraitch, Farlan S ^a   Mason, Chris ^a   Wall, Ivan B ^a,c   Caldwell, Maeve A ^b  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

^c Dankook University   (South Korea)

Author keywords

embryonic stem cell; feeder free; HIF1A; hypoxia; induced pluripotent stem cell; mouse embryonic fibroblast

Indexed keywords

TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR HIF1A; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL DIFFERENTIATION; CELL GROWTH; CELL HYPOXIA; CELL METABOLISM; CELL VIABILITY; CONTROLLED STUDY; EMBRYO; FEEDER CELL; FIBROBLAST CULTURE; GERM LAYER; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOCYTOCHEMISTRY; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN STABILITY; STEM CELL EXPANSION; WESTERN BLOTTING;

ANIMALS; BIOLOGICAL MARKERS; CELL CULTURE TECHNIQUES; CELL EXTRACTS; CELL HYPOXIA; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; EMBRYO, MAMMALIAN; FIBROBLASTS; HUMANS; HYPOXIA-INDUCIBLE FACTOR 1, ALPHA SUBUNIT; MICE; PLURIPOTENT STEM CELLS; PROTEIN STABILITY;

EID: 84866109199     PISSN: 17460751     EISSN: 1746076X     Source Type: Journal    
DOI: 10.2217/rme.12.55     Document Type: Article

References (34)

1. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al. Embryonic stem cell lines derived from human blastocysts. Science 282(5391), 1145-1147 (1998). (Pubitemid 28516247)
2. Fischer B, Bavister BD. Oxygen tension in the oviduct and uterus of rhesus monkeys, hamsters and rabbits. J. Reprod. Fertil. 99(2), 673-679 (1993). (Pubitemid 24052018)
3. Forsyth NR, Musio A, Vezzoni P, Simpson AH, Noble BS, McWhir J. Physiologic oxygen enhances human embryonic stem cell clonal recovery and reduces chromosomal abnormalities. Cloning Stem Cells 8(1), 16-23 (2006).
4. Prasad SM, Czepiel M, Cetinkaya C et al. Continuous hypoxic culturing maintains activation of Notch and allows long-term propagation of human embryonic stem cells without spontaneous differentiation. Cell Prolif. 42(1), 63-74 (2009).
5. Millman JR, Tan JH, Colton CK. The effects of low oxygen on self-renewal and differentiation of embryonic stem cells. Curr. Opin. Organ Transplant. 14(6), 694-700 (2009).
6. Forristal CE, Wright KL, Hanley NA, Oreffo ROC, Houghton FD. Hypoxia inducible factors regulate pluripotency and proliferation in human embryonic stem cells cultured at reduced oxygen tensions. Reproduction 139(1), 85-97 (2010).
7. Covello KL, Kehler J, Yu H et al. HIF-2alpha regulates Oct-4: Effects of hypoxia on stem cell function, embryonic development, and tumor growth. Genes Dev. 20(5), 557-570 (2006). (Pubitemid 43345322)
8. Ezashi T, Das P, Roberts RM. Low O2 tensions and the prevention of differentiation of hES cells. Proc. Natl Acad. Sci. USA 102(13), 4783-4788 (2005). (Pubitemid 40471531)
9. Szablowska-Gadomska I, Zayat V, Buzanska L. Influence of low oxygen tensions on expression of pluripotency genes in stem cells. Acta Neurobiol. Exp. (Wars.) 71(1), 86-93 (2011).
10. Stacpoole SRL, Bilican B, Webber DJ et al. Efficient derivation of NPCs, spinal motor neurons and midbrain dopaminergic neurons from hESCs at 3% oxygen. Nat. Protoc. 6(8), 1229-1240 (2011).
11. Santilli G, Lamorte G, Carlessi L et al. Mild hypoxia enhances proliferation and multipotency of human neural stem cells. PLoS One 5(1), e8575 (2010).
12. Yoshida Y, Takahashi K, Okita K, Ichisaka T, Yamanaka S. Hypoxia enhances the generation of induced pluripotent stem cells. Cell Stem Cell 5(3), 237-241 (2009).
13. Mondragon-Teran P, Lye GJ, Veraitch FS. Lowering oxygen tension enhances the differentiation of mouse embryonic stem cells into neuronal cells. Biotechnol. Prog. 25(5), 1480-1488 (2009).
14. Kim T-S, Misumi S, Jung C-G et al. Increase in dopaminergic neurons from mouse embryonic stem cell-derived neural progenitor/stem cells is mediated by hypoxia inducible factor-1alpha. J. Neurosci. Res. 86(11), 2353-2362 (2008).
15. Studer L, Csete M, Lee SH et al. Enhanced proliferation, survival, and dopaminergic differentiation of CNS precursors in lowered oxygen. J. Neurosci. 20(19), 7377-7383 (2000).
16. McElroy SL, Reijo Pera RA. Preparation of mouse embryonic fibroblast feeder cells for human embryonic stem cell culture. CSH Protoc. doi:10.1101/pdb.prot5041 (2008) (Epub ahead of print).
17. Diecke S, Quiroga-Negreira A, Redmer T, Besser D. FGF2 signaling in mouse embryonic fibroblasts is crucial for self-renewal of embryonic stem cells. Cells Tissues Organs 188(1-2), 52-61 (2008). (Pubitemid 351962229)
18. Ludwig TE, Levenstein ME, Jones JM et al. Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol. 24(2), 185-187 (2006). (Pubitemid 43221701)
19. International Stem Cell Initiative Consortium; Akopian V, Andrews PW, Beil S et al. Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells. In Vitro Cell. Dev. Biol. Anim. 46(3-4), 247-258 (2010).
20. Nelson DL, Cox MM. Lehninger Principles of Biochemistry (5th Edition). WH Freeman, NY, USA (2008).
21. Joannides AJ, Fiore-Hériché C, Battersby AA et al. A scaleable and defined system for generating neural stem cells from human embryonic stem cells. Stem Cells 25(3), 731-737 (2007). (Pubitemid 46354233)
22. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65(1-2), 55-63 (1983). (Pubitemid 14203433)
23. Stacpoole SRL, Bilican B, Webber DJ et al. Derivation of neural precursor cells from human ES cells at 3% O2 is efficient, enhances survival and presents no barrier to regional specification and functional differentiation. Cell Death Differ. 18(6), 1016-1023 (2011).
24. Cameron CM, Harding F, Hu W-S, Kaufman DS. Activation of hypoxic response in human embryonic stem cell-derived embryoid bodies. Exp. Biol. Med. (Maywood) 233(8), 1044-1057 (2008).
25. Varma S, Rakusan K, Cowan J, Hetenyi G. Stimulation of glucose production by hypoxia in newborn dogs. Can. J. Physiol. Pharm. 51(6), 464-471 (1973).
26. Jones C, Ritchie J, Walker D. The effects of hypoxia on glucose-turnover in the fetal sheep. J. Dev. Physiol. 5(4), 223-235 (1983).
27. Bristow J, Rudolph AM, Itskovitz J, Barnes R. Hepatic oxygen and glucose metabolism in the fetal lamb. Response to hypoxia. J. Clin. Invest. 71(5), 1047-1061 (1983). (Pubitemid 13098892)
28. Déry M-AC, Michaud MD, Richard DE. Hypoxia-inducible factor 1: Regulation by hypoxic and non-hypoxic activators. Int. J. Biochem. Cell Biol. 37(3), 535-540 (2005). (Pubitemid 40022930)
29. Bruick RK. Oxygen sensing in the hypoxic response pathway: Regulation of the hypoxia-inducible transcription factor. Genes Dev. 17(21), 2614-2623 (2003). (Pubitemid 37363065)
30. Draper JS, Moore HD, Ruban LN, Gokhale PJ, Andrews PW. Culture and characterization of human embryonic stem cells. Stem Cells Dev. 13(4), 325-336 (2004). (Pubitemid 39223282)
31. Catalina P, Montes R, Ligero G et al. Human ESCs predisposition to karyotypic instability: Is a matter of culture adaptation or differential vulnerability among hESC lines due to inherent properties? Mol. Cancer 7, 76 (2008).
32. Draper JS, Smith K, Gokhale P et al. Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat. Biotechnol. 22(1), 53-54 (2004). (Pubitemid 38057956)
33. Rajala K, Hakala H, Panula S et al. Testing of nine different xeno-free culture media for human embryonic stem cell cultures. Hum. Reprod. 22(5), 1231-1238 (2007). (Pubitemid 47062789)
34. Kusuda Furue M, Tateyama D, Kinehara M, Na J, Okamoto T, Sato JD. Advantages and difficulties in culturing human pluripotent stem cells in growth factor-defined serum-free medium. In Vitro Cell. Dev. Biol. Anim. 46(7), 573-576 (2010).