Efficient heterologous expression and secretion in Aspergillus oryzae of a llama variable heavy-chain antibody fragment VHH against EGFR

Applied Microbiology and Biotechnology

Volumn 96, Issue 1, 2012, Pages 81-88

  Okazaki, Fumiyoshi ^a   Aoki, Jun Ichi ^a   Tabuchi, Soichiro ^a   Tanaka, Tsutomu ^a   Ogino, Chiaki ^a   Kondo, Akihiko ^a  

^a KOBE UNIVERSITY   (Japan)

Author keywords

Antibody; Aspergillus oryzae; EGFR; VHH

Indexed keywords

ANTI-EGFR; ASPERGILLUS ORYZAE; CULTURE MEDIUM; EGFR; EPIDERMAL GROWTH FACTOR RECEPTORS; FILAMENTOUS FUNGI; HEAVY-CHAIN ANTIBODY FRAGMENTS; HETEROLOGOUS EXPRESSION; HIGH AFFINITY; N-TERMINALS; RHIZOPUS ORYZAE LIPASE; SIGNAL SEQUENCE; TAKA-AMYLASE A;

AMINO ACIDS; ANTIBODIES;

ASPERGILLUS;

AMYLASE; ANTIBODY; EPIDERMAL GROWTH FACTOR RECEPTOR; HEAVY CHAIN ANTIBODY FRAGMENT; UNCLASSIFIED DRUG;

ANTIBODY; ENZYME ACTIVITY; FUNGUS; GENE EXPRESSION; SECRETION;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; ASPERGILLUS ORYZAE; CIRCULAR DICHROISM; CONTROLLED STUDY; CULTURE MEDIUM; HEAVY CHAIN; IMMUNOFLUORESCENCE TEST; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; WESTERN BLOTTING;

ANIMALS; ASPERGILLUS ORYZAE; CAMELIDS, NEW WORLD; LIPASE; PROTEIN SORTING SIGNALS; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECOMBINANT PROTEINS; RHIZOPUS; SINGLE-DOMAIN ANTIBODIES;

ASPERGILLUS ORYZAE; FUNGI; LAMA (MAMMAL); RHIZOPUS ORYZAE;

EID: 84866053242     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4158-1     Document Type: Article

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