The walking dead: Is hydroethidine a suitable viability dye for intra-erythrocytic Plasmodium falciparum?

Parasitology International

Volumn 61, Issue 4, 2012, Pages 731-734

  Engelbrecht, Dewaldt ^a   Coetzer, Thérèsa Louise ^a  

^a UNIVERSITY OF THE WITWATERSRAND   (South Africa)

Author keywords

Hydroethidine; Plasmodium falciparum; Thiazole orange; Viability

Indexed keywords

HYDROETHIDINE; THIAZOLE ORANGE;

ARTICLE; CONTROLLED STUDY; DNA FRAGMENTATION; FLOW CYTOMETRY; IN VITRO STUDY; NONHUMAN; PARASITE DEVELOPMENT; PARASITE VIABILITY; PARASITEMIA; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL;

ANIMALS; COLORING AGENTS; DNA FRAGMENTATION; FLOW CYTOMETRY; PARASITE LOAD; PHENANTHRIDINES; PLASMODIUM FALCIPARUM; STAINING AND LABELING;

PLASMODIUM FALCIPARUM;

EID: 84865986255     PISSN: 13835769     EISSN: 18730329     Source Type: Journal    
DOI: 10.1016/j.parint.2012.07.002     Document Type: Article

References (17)

1. Wyatt C.R., Goff W., Davis W.C. A flow cytometric method for assessing viability of intraerythrocytic hemoparasites. Journal of Immunological Methods 1991, 140:23-30.
2. da Cruz L.N., Alves E., Leal M.T., Juliano M.A., Rosenthal P.J., Juliano L., et al. FRET peptides reveal differential proteolytic activation in intraerythrocytic stages of the malaria parasites Plasmodium berghei and Plasmodium yoelii. International Journal for Parasitology 2011, 41:363-372.
3. Elloso M.M., Van Der Heyde H.C., Vande Waa J.A., Manning D.D., Weidanz W.P. Inhibition of Plasmodium falciparum in vitro by human γδ T cells. Journal of Immunology 1994, 153:1187-1194.
4. Jouin H., Daher W., Khalife J., Ricard I., Puijalon O.M., Capron M., et al. Double staining of Plasmodium falciparum nucleic acids with hydroethidine and thiazole orange for cell cycle stage analysis by flow cytometry. Cytometry. Part A 2004, 57A:34-38.
5. Van der Heyde H.C., Elloso M.M., Vande Waa J., Schell K., Weidanz W.P. Use of hydroethidine and flow cytometry to assess the effects of leukocytes on the malarial parasite Plasmodium falciparum. Clinical and Diagnostic Laboratory Immunology 1995, 2(4):417-425.
6. Rost F.W.D. Fluorescence microscopy 1995, Cambridge University Press, New York.
7. Zielonka J., Kalyanaraman B. Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: another inconvenient truth. Free Radical Biology & Medicine 2010, 48:983-1001.
8. Trager W., Jensen J. Human malaria parasites in continuous culture. Science 1976, 193:673-675.
9. Mphande F., Nilsson S., Bolad A. Culturing of erythrocytic asexual stages of Plasmodium falciparum and P. vivax. Methods in malaria research. Virginia: MR4/ATCC 2008, 1-3. K. Moll, I. Ljungström, H. Perlmann, A. Scherf, M. Wahlgren (Eds.).
10. Lambros C., Vanderberg J.P. Synchronization of Plasmodium falciparum erythrocytic stages in culture. Journal of Parasitology 1979, 65(3):418-420.
11. Schulze D.L.C., Makgatho E.M., Coetzer T.L., Louw A.I., Van Rensburg C.E.J., Visser L. Development and application of a modified flow cytometric procedure for rapid in vitro quantitation of malaria parasitaemia. South African Journal of Science 1997, 93(4):156-158.
12. Oakley M.S.M., Kumar S., Anantharaman V., Zheng H., Mahajan B., Haynes J.D., et al. Molecular factors and biochemical pathways induced by febrile temperature in intraerythrocytic Plasmodium falciparum parasites. Infection and Immunity 2007, 75(4):2012-2025.
13. Miller L.H., Baruch D.I., Marsh K., Doumbo O.K. The pathogenic basis of malaria. Nature 2002, 415:673-679.
14. Swannell R.P.J., Caplin R., Nedwell D.B., Williamson F.A. An investigation of hydroethidine as a fluorescent vital stain for prokaryotes. FEMS Microbiology Ecology 1992, 101:173-182.
15. Papapostolou I., Patsoukis N., Georgiou C.D. The fluorescence detection of superoxide radical using hydroethidine could be complicated by the presence of heme proteins. Analytical Biochemistry 2004, 332:290-298.
16. Gomes A., Fernandes E., Lima J.L.F.C. Fluorescence probes used for detection of reactive oxygen species. Journal of Biochemical and Biophysical Methods 2005, 65:45-80.
17. Rothe G., Valet G. Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2',7'-dichlorofluorescin. Journal of Leukocyte Biology 1990, 47:440-448.