Real-time PCR detection and quantification of emerging waterborne pathogens (EWPs) and antibiotic resistance genes (ARGs) in the downstream area of Jiulong River

Huanjing Kexue/Environmental Science

Volumn 33, Issue 8, 2012, Pages 2685-2690

  Wang, Qing ^a   Lin, Hui Rong ^a   Zhang, Shu Ting ^a   Yu, Xin ^a  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

Antibiotic resistance genes(ARGs); Drinking water; Emerging waterborne pathogens(EWPs); Jiulong River; Real time PCR

Indexed keywords

BACTERIAL DNA; DRINKING WATER;

ANTIBIOTIC RESISTANCE; ARTICLE; DRUG EFFECT; GENETICS; ISOLATION AND PURIFICATION; LEGIONELLA PNEUMOPHILA; MICROBIOLOGY; PSEUDOMONAS AERUGINOSA; REAL TIME POLYMERASE CHAIN REACTION; RIVER; SALMONELLA ENTERICA;

DNA, BACTERIAL; DRINKING WATER; DRUG RESISTANCE, BACTERIAL; LEGIONELLA PNEUMOPHILA; PSEUDOMONAS AERUGINOSA; REAL-TIME POLYMERASE CHAIN REACTION; RIVERS; SALMONELLA ENTERICA; WATER MICROBIOLOGY;

EID: 84865973886     PISSN: 02503301     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. 2011, 37(8): 1-5.
2. Behravesh C B, Mody R K, Jungk J, et al. 2008 outbreak of Salmonella Saintpaul infections associated with raw produce[J]. The New England Journal of Medicine, 2011, 364(10): 918-927.
3. Mackie R I, Koike S, Krapac I, et al. Tetracycline residues and tetracycline resistance genes in groundwater impacted by swine production facilities[J]. Animal Biotechnology, 2006, 17(2): 157-176.
4. Tennstedt T, Szczepanowski R, Braun S, et al. Occurrence of integron-associated resistance gene cassettes located on antibiotic resistance plasmids isolated from a wastewater treatment plant[J]. FEMS Microbiology Ecology, 2003, 45(3): 239-252.
5. Heuer H, Szczepanowski R, Schneiker S, et al. The complete sequences of plasmids pB2 and pB3 provide evidence for a recent ancestor of the IncP-1β group without any accessory genes[J]. Microbiology, 2004, 150(11): 3591-3599.
6. Poppe C, Martin L, Muckle A, et al. Characterization of antimicrobial resistance of Salmonella Newport isolated from animals, the environment, and animal food products in Canada[J]. Canadian Journal of Veterinary Research, 2006, 70(2): 105-114.
7. Thompson S A, Maani E V, Lindell A H, et al. Novel tetracycline resistance determinant isolated from an environmental strain of Serratia marcescens[J]. Applied and Environmental Microbiology, 2007, 73(7): 2199-2206.
8. Lee Y J, Han H S, Seong C N, et al. Distribution of genes coding for aminoglycoside avetyltransferases in gentamicin resistant bacteria isolated from aquatic environment[J]. Journal of Microbiology, 1998, 36(4): 249-255.
9. 2008, 28(8): 1499-1505.
10. 2010, 41(8): 148-155.
11. Zhang S T, Wei B, Yu X, et al. Development of pretreatment protocol for DNA extraction from biofilm attached to biologic activated carbon (BAC) granules[J]. Frontiers of Environmental Science and Engineering in China, 2010, 4(4): 459-465.
12. Pruden A, Pei R T, Storteboom H, et al. Antibiotic resistance genes as emerging contaminants: studies in northern Colorado[J]. Environmental Science and Technology, 2006, 40(23): 7445-7450.
13. Tennstedt T, Szczepanowski R, Krahn I, et al. Sequence of the 68, 869 bp IncP-1α plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements[J]. Plasmid, 2005, 53(3): 218-238.
14. Ng L K, Martin I, Alfa M, et al. Multiplex PCR for the detection of tetracycline resistant genes[J]. Molecular and Cellular Probes, 2001, 15(4): 209-215.
15. Bell J M, Paton J C, Turnidge J. Emergence of vancomycin-resistant enterococci in Australia: phenotypic and genotypic characteristics of isolates[J]. Journal of Clinical Microbiology, 1998, 36(8): 2187-2190.
16. Jensen L B, Frimodt-Möller N, Aarestrup F M. Presence of erm gene classes in gram-positive bacteria of animal and human origin in Denmark[J]. FEMS Microbiology Letters, 1999, 170(1): 151-158.
17. Kong R Y C, Lee S K Y, Law T W F, et al. Rapid detection of six types of bacterial pathogens in marine waters by multiplex PCR[J]. Water Research, 2002, 36(11): 2802-2812.
18. Ibekwe A M, Watt P M, Grieve C M, et al. Multiplex fluorogenic real-time PCR for detection and quantification of Escherichia coli O157:H7 in dairy wastewater wetlands[J]. Applied and Environmental Microbiology, 2002, 68(10): 4853-4862.
19. Maheux A F, Picard F J, Boissinot M, et al. Analytical comparison of nine PCR primer sets designed to detect the presence of Escherichia coli/Shigella in water samples[J]. Water Research, 2009, 43(12): 3019-3028.
20. Pirnay J P, De Vos D, Duinslaeger L, et al. Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid 'real-time' polymerase chain reaction[J]. Critical Care, 2000, 4(4): 255-262.
21. Wellinghausen N, Frost C, Marre R. Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR[J]. Applied and Environmental Microbiology, 2001, 67(9): 3985-3993.
22. Levy H, Diallo S, Tennant S M, et al. PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella isolates from the blood of patients with clinical enteric fever[J]. Journal of Clinical Microbiology, 2008, 46(5): 1861-1866.
23. Ramesh A, Padmapriya B P, Chrashekar A, et al. Application of a convenient DNA extraction method and multiplex PCR for the direct detection of Staphylococcus aureus and Yersinia enterocolitica in milk samples[J]. Molecular and Cellular Probes, 2002, 16(4): 307-314.