Quantitation of pseudomonas aeruginosa in wound biopsy samples: From bacterial culture to rapid 'real-time' polymerase chain reaction

Critical Care

Volumn 4, Issue 4, 2000, Pages 255-261

  Pirnay, Jean Paul ^a,b,d   De Vos, Daniel ^b,c,d   Duinslaeger, Luc ^a   Reper, Pascal ^a   Vandenvelde, Christian ^a   Cornelis, Pierre ^b,d   Vanderkelen, Alain ^a  

^a QUEEN ASTRID MILITARY HOSPITAL   (Belgium)

^b DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^c INNOGENETICS   (Belgium)

^d VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

Author keywords

Burn wound; Polymerase chain reaction; Pseudomonas aeruginosa; Quantitation; Sepsis

Indexed keywords

ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BIOPSY; CLINICAL ARTICLE; COLONY FORMING UNIT; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN TISSUE; INTENSIVE CARE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SEPSIS;

EID: 0033860887     PISSN: 13648535     EISSN: None     Source Type: Journal    
DOI: 10.1186/cc702     Document Type: Article

References (44)

1. Pruitt BA Jr, McManus AT, Kim SH, Goodwin CW: Burn wound infections: current status. World J Surg 1998, 22:135-145.
2. Lee JJ, Marvin JA, Heimbach DM, Grube BJ, Engrave LH: Infection control in a burn center. J Burn Care Rehabil 1992, 11:575-580.
3. McManus AT, Mason AD Jr, McManus WF, Pruitt BA Jr: Twenty-five year review of Pseudomonas aeruginosa bacteremia in a burn center. Eur J Clin Microbiol 1985, 4:219-223.
4. Teplitz C, Davis D, Mason AD Jr, Moncrief JA: Pseudomonas burn wound sepsis. I. Pathogenesis of experimental Pseudomonas burn wound sepsis. J Surg Res 1964, 4:200-222.
5. Moncrief JA, Lindberg RB, Switzer WE, Pruitt BA Jr: Use of topical antibacterial therapy in the treatment of the burn wound. Arch Surg 1966, 92:558-565.
6. Perez-Cappelano R, Manelli JC, Palayret D, Carlin G, Echinard C, Jouglard JP: Evaluation of the septicaemic risk by a quantitative study of the cutaneous flora in patients with burns. Burns 1976, 3:42-45.
7. Lawrence JC, Lilly HA: A quantitative method for investigating the bacteriology of the skin: its applicability to burns. Br J Exp Pathol 1972, 53:550-558.
8. Loebl EC, Marvin JA, Heck EL, Curreri, PW, Baxter CR: The method of quantitative burn-wound biopsy cultures and its routine use in the care of the burned patient Am J Clin Pathol 1974, 61:20-24.
9. McManus WF, Goodwin CW, Mason AD Jr, Pruitt BA Jr: Burn wound infection. J Trauma 1981, 21:753-756.
10. Taddonio TE, Thomson PD, Tait MJ, Prasad JK, Feller I: Rapid quantification of bacterial and fungal growth in burn wounds: biopsy homogenate Gram stain versus microbial culture results. Burns 1988, 14:180-184.
11. Bharadwaj R, Joshi BN, Phadke SA: Assessment of burn wound sepsis by swab, full thickness biopsy culture and blood culture: a comparative study. Burns 1983, 10:124-126.
12. Heininger A, Binder M, Schmidt S, Unertl K, Botzenhart K, Döring G: PCR and blood culture for detection of Escherichia coli bacteremia in rats. J Clin Microbiol 1999, 37:2479-2482.
13. McManus AT, Kim SH, McManus WF, Mason AD Jr, Pruitt BA Jr: Comparison of quantitative microbiology and histopathology in divided burn-wound biopsy specimens. Arch Surg 1987, 122: 74-76.
14. Masuda N, Sakagawa E, Ohya S: Outer membrane proteins responsible for multiple drug resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother 1995, 39:645-649.
15. Nikaido H: Prevention of drug access to bacterial targets: permeability barriers and active efflux. Science 1994, 264:382-388.
16. Burdon DW, Whitby JL: Contamination of hospital disinfectants with Pseudomonas species. Br Med J 1967, 2:153-155.
17. Parrott PL, Terry PM, Whitworth EN, Frawley LW, Coble RS, Wachsmuth IK, McGowan JE Jr: Pseudomonas aeruginosa peritonitis associated with contaminated poloxamer-iodine solution. Lancet 1982, ii:683-685.
18. Craven DE, Steger KA: Nosocomial pneumonia in mechanically ventilated adult patients: epidemiology and prevention in 1996. Semin Respir Infect 1996, 11:32-53.
19. Koch C, Høiby N: Pathogenesis of cystic fibrosis. Lancet 1993, 341: 1065-1069.
20. Cornelis P, Bouia A, Belarbi A, Guyonvarch A, Kammerer B, Hannaert V, Hubert JC: Cloning and analysis of the gene for the major outer membrane lipoprotein from Pseudomonas aeruginosa. Mol Microbiol 1989, 3:421-428.
21. Lim A Jr, De Vos D, Brauns M, Mossialos D, Gaballa A, Qing D, Cornelis P: Molecular and immunological characterization of OprL, the 18-kDa outer membrane peptidoglycan-associated (PAL) lipoprotein of Pseudomonas aeruginosa. Microbiology 1997, 143:1709-1716.
22. De Vos D, Lim A Jr, Pirnay JP, Struelens M, Vandenvelde C, Duinslaeger L, Vanderkelen A, Cornelis P: Direct detection of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprl and oprL. J Clin Microbiol 1997, 35:1295-1299.
23. Holland PM, Abramson RD, Watson R, Gelfand DH: Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci USA 1991, 88:7276-7280.
24. Tyagi S, Kramer FR: Molecular beacons: probes that fluoresce upon hybridization. Nature Biotechnol 1996, 14:303-308.
25. Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP: Continuous fluorescence monitoring of rapid cycle DNA amplifications. BioTechniques 1997, 22:130-138.
26. Nazarenko IA, Bhatnagar SK, Hohman RJ: A closed tube format for amplification and detection of DNA based on energy transfer. Nucleic Acids Res 1997, 25:2516-2521.
27. Whitcombe D, Theaker J, Guy SP, Brown T, Little S: Detection of PCR products using self-probing amplicons and fluorescence. Nafure Biotechnol 1999, 17:804-807.
28. ™: a microvolume multisample fluorimeter with rapid temperature control. BioTechniques 1997, 22:176-181.
29. Livak KJ, Flood SJA, Marmaro J, Giusti W, Deetz K: Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 1995, 4:357-362.
30. Desjardin LE, Chen Y, Perkins MD, Teixeira L, Cave MD, Eisenach KD: Comparison of the ABI 7700 system (TaqMan) and competitive PCR for quantification of IS6110 DNA in sputum during treatment of tuberculosis. J Clin Microbiol 1998, 36:1964-1968.
31. Matsumoto T, Tanaka M, Ogata N, Mizunoe Y, Takahashi K, Kumazawa J: Significance of urinary endotoxin concentration in patients with urinary tract infection. Urol Res 1991, 19:293-295.
32. Fischer GW, Smith LP, Hemming VG, Longfield R, Valdes-Dapena AA, Lopreiato JO: Avoidance of false-negative blood culture results by rapid detection of pneumococcal antigen. JAMA 1984, 252:1742-1743.
33. Razin S: DNA probes and PCR in diagnosis of mycoplasma infections. Mol Cell Probes 1994, 8:497-511.
34. Oberst RD, Hays MP, Bohra LK, Phebus RK, Yamashiro CT, Paszko-Kolva C, Flood SJ, Sargeant JM, Gillespie JR: PCR-based DNA amplification and presumptive detection of Escherichia coli O157:H7 with an internal fluorogenic probe and the 5′ nuclease (TaqMan) assay. Appl Environ Microbiol 1998, 64:3389-3396.
35. Bieche I, Olivi M, Champeme MH, Vidaud D, Lidereau R, Vidaud M: Novel approach to quantitative polymerase chain reaction using real-time detection: application to the detection of gene amplification in breast cancer. Int J Cancer 1998, 78:661-666.
36. Laurendeau I, Bahuau M, Vodovar N, Larramendy C, Olivi M, Bieche I, Vidaud M, Vidaud D: TaqMan PCR-based gene dosage assay for predictive testing in individuals from a cancer family with INK4 locus haploinsufficiency. Clin Chem 1999, 45:982-986.
37. Mensink E, van de Locht A, Schattenberg A, Linders E, Schaap N, Geurts van Kessel A, De Witte T: Quantitation of minimal residual disease in Philadelphia chromosome positive chronic myeloid leukemia using real-time quantitative RT-PCR. Br J Haematol 1998, 102:768-774.
38. Von Ahsen N, Schutz E, Armstrong VW, Oellerich M: Rapid detection of prothrombotic mutations of prothrombin (G20210A), factor V (G1691A) and methylenetetrahydrofolate reductase (C677T) by real-time fluorescence PCR with the LightCycler. Clin Chem 1999, 45:694-696.
39. Lewin SR, Vesanen M, Kostrikis L, Hurley A, Duran M, Zhang L, Ho DD, Markowitz M: Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1-infected individuals on prolonged effective antiretroviral therapy. J Virol 1999, 73:6099-6103.
40. Pahl A, Kuhlbrandt U, Brune K, Rollinghoff M, Gessner A: Quantitative detection of Borrelia burgdorferi by real-time PCR. J Clin Microbiol 1999, 37:1958-1963.
41. Martell M, Gomez J, Esteban JI, Sauleda S, Quer J, Cabot B, Esteban R, Guardia J: High-throughput real-time reverse transcription-PCR quantitation of hepatitis C virus RNA. J Clin Microbiol 1999, 37: 327-332.
42. Kimura H, Morita M, Yabuta Y, Kuzushima K, Kato K, Kojima S, Matsuyama T, Morishima T: Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay. J Clin Microbiol 1999, 37: 132-136.
43. Doern G, Vautour R, Gaudet M, Levy B: Clinical impact of rapid in vitro susceptibility testing and bacterial identification. J Clin Microbiol 1994, 32:1757-1762.
44. Barenfanger J, Drake C, Kacich G: Clinical and financial benefits of rapid bacterial identification and antimicrobial susceptibility testing. J Clin Microbiol 1999, 37:1415-1418.