In-field implementation of a recombinant factor C assay for the detection of lipopolysaccharide as a biomarker of extant life within glacial environments

Biosensors

Volumn 2, Issue 1, 2012, Pages 83-100

  Barnett, Megan J ^a   Wadham, Jemma L ^b   Jackson, Miriam ^c   Cullen, David C ^a  

^a CRANFIELD UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

^c NORWEGIAN WATER RESOURCES AND ENERGY DIRECTORATE   (Norway)

Author keywords

Cryosphere; Endotoxin; Lipopolysaccharide; Portable life detection; Pyrogen; Recombinant factor c; Subglacial

Indexed keywords

BACTERIA; BIOGEOCHEMISTRY; CELL MEMBRANES; ECOLOGY; GLACIAL GEOLOGY;

CRYOSPHERE; ENDOTOXIN; IN-FIELD; LIFE DETECTION; LIPOPOLYSACCHARIDES; MICROBIAL COMMUNITIES; PORTABLE LIFE DETECTION; PYROGEN; RECOMBINANT FACTOR C; SUBGLACIAL;

BIODIVERSITY;

BACTERIUM LIPOPOLYSACCHARIDE; BIOLOGICAL MARKER; ENDOTOXIN;

ARTICLE; ASSAY; BACTERIUM DETECTION; BIOGEOCHEMICAL CYCLE; CRYOSPHERE; FLUOROMETER; GLACIER; GRAM NEGATIVE BACTERIUM;

BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 84865845844     PISSN: None     EISSN: 20796374     Source Type: Journal    
DOI: 10.3390/bios2010083     Document Type: Article

References (33)

1. Aycicek, H.; Oguz, U.; Karci, K. Comparison of results of ATP bioluminescence and traditional hygiene swabbing methods for the determination of surface cleanliness at a hospital kitchen. Int. J. Hyg. Environ. Health 2006, 209, 203-206.
2. Lipscomb, I.P.; Sihota, A.K.; Botham, M.; Harris, K.L.; Keevil, C.W. Rapid method for the sensitive detection of protein contamination on surgical instruments. J. Hosp. Infect. 2006, 62, 141-148.
3. Rasmussen, J.P.; Giglio, S.; Monis, P.T.; Campbell, R.J.; Saint, C.P. Development and field testing of a real-time PCR assay for cylindrospermopsin-producing cyanobacteria. J. Appl. Microbiol. 2008, 104, 1503-1515.
4. Davidson, C.A.; Griffith, C.J.; Peters, A.C.; Fielding, L.M. Evaluation of two methods for monitoring surface cleanliness-ATP bioluminescence and traditional hygiene swabbing. Luminescence 1999, 14, 33-38.
5. Sharp, M.; Parkes, J.; Cragg, B.; Fairchild, I.J.; Lamb, H.; Tranter, M. Widespread bacterial populations at glacier beds and their relationship to rock weathering and carbon cycling. Geology 1999, 27, 107-110.
6. Skidmore, M.L.; Foght, J.M.; Sharp, M.J. Microbial life beneath a High Arctic glacier. Appl. Environ. Microbiol. 2000, 66, 3214-3220.
7. Foght, J.; Aislabie, J.; Turner, S.; Brown, C.E.; Ryburn, J.; Saul, D.J.; Lawson, W. Culturable bacteria in subglacial sediments and ice from two Southern Hemisphere glaciers. Microb. Ecol. 2004, 47, 329-340.
8. Gaidos, E.; Lanoil, B.; Thorsteinsson, T.; Graham, A.; Skidmore, M.; Han, S.K.; Rust, T.; Popp, B. A viable microbial community in a subglacial volcanic crater lake, Iceland. Astrobiology 2004, 4, 327-344.
9. Mikucki, J.A.; Foreman, C.M.; Sattler, B.; Berry Lyons, W.; Priscu, J.C. Geomicrobiology of Blood Falls: An iron-rich saline discharge at the terminus of the Taylor Glacier, Antarctica. Aquat. Geochem. 2004, 10, 199-220.
10. Skidmore, M.; Anderson, S.P.; Sharp, M.; Foght, J.; Lanoil, B.D. Comparison of microbial community compositions of two subglacial environments reveals a possible role for microbes in chemical weathering processes. Appl. Environ. Microbiol. 2005, 71, 6986-6997.
11. Siegert, M.J.; Behar, A.; Bentley, M.; Blake, D.; Bowden, S.; Christoffersen, P.; Cockell, C.; Corr, H.; Cullen, D.C.; Edwards, H.; et al. Exploration of Ellsworth Subglacial Lake: A concept paper on the development, organisation and execution of an experiment to explore, measure and sample the environment of a West Antarctic subglacial lake: The Lake Ellsworth Consortium. Rev. Environ. Sci. Biotechnol. 2007, 6, 161-179.
12. Hodson, A.; Anesio, A.M.; Tranter, M.; Fountain, A.; Osborn, M.; Priscu, J.; Laybourn-Parry, J.; Sattler, B. Glacial ecosystems. Ecol. Monogr. 2008, 78, 41-67.
13. Watson, S.W.; Novitsky, T.J.; Quinby, H.L.; Valois, F.W. Determination of bacterial number and biomass in the marine environment. Appl. Environ. Microbiol. 1977, 33, 940-946.
14. Xiang, S.; Yao, T.; An, L.; Xu, B.; Wang, J. 16S rRNA sequences and differences in bacteria isolated from the Muztag Ata glacier at increasing depths. Appl. Environ. Microbiol. 2005, 71, 4619-4627.
15. Brinkmeyer, R.; Knittel, K.; Jurgens, J.; Weyland, H.; Amann, R.; Helmke, E. Diversity and structure of bacterial communities in Arctic versus Antarctic pack ice. Appl. Environ. Microbiol. 2003, 69, 6610-6619.
16. Mosier, A.C.; Murray, A.E.; Fritsen, C.H. Microbiota within the perennial ice cover of Lake Vida, Antarctica. FEMS Microbiol. Ecol. 2007, 59, 274-288.
17. Amato, P.; Hennebelle, R.; Magand, O.; Sancelme, M.; Delort, A.M.; Barbante, C.; Boutron, C.; Ferrari, C. Bacterial characterization of the snow cover at Spitzberg, Svalbard. FEMS Microbiol. Ecol. 2007, 59, 255-264.
18. Bang, F.B. A bacterial disease of Limulus polyphemus. B. Johns Hopkins Hosp. 1956, 98, 325-351.
19. Levin, J.; Tomasulo, P.A.; Oser, R.S. Detection of endotoxin in human blood and demonstration of an inhibitor. J. Lab. Clin. Med. 1970, 75, 903-911.
20. Steele, A.; Schweizer, M.; Amundsen, H.E.F.; Wainwright, N. In-Field Testing of Life Detection Instruments and Protocols in a Mars Analogue Arctic Environment. In Proceedings of the 35th Lunar and Planetary Science Conference, League City, TX, USA, 15-19 March 2004.
21. La Ferla, R.; Lo Giudice, A.; Maimone, G. Morphology and LPS content for the estimation of marine bacterioplankton biomass in the Ionian Sea. Sci. Mar. 2004, 68, 23-31.
22. Karl, D.M.; Bird, D.F.; Björkman, K.; Houlihan, T.; Shackelford, R.; Tupas, L. Microorganisms in the accreted ice of Lake Vostok, Antarctica. Science 1999, 286, 2144-2147.
23. Nakamura, T.; Morita, T.; Iwanaga, S. Lipopolysaccharide-sensitive serine-protease zymogen (factor C) found in Limulus hemocytes. Isolation and characterization. Eur. J. Biochem. 1986, 154, 511-521.
24. Ding, J.L.; Ho, B. A new era in pyrogen testing. Trends Biotechnol. 2001, 19, 277-281.
25. Lappegard, G.; Kohler, J.; Jackson, M.; Hagen, J.O. Characteristics of subglacial drainage systems deduced from load-cell measurements. J. Glaciol. 2006, 52, 137-148.
26. Douwes, J.; Versloot, P.; Hollander, A.; Heederik, D.; Doekes, G. Influence of various dust sampling and extraction methods on the measurement of airborne endotoxin. Appl. Environ. Microbiol. 1995, 61, 1763-1769.
27. Holzheimer, R. The significance of endotoxin release in experimental and clinical sepsis in surgical patients-Evidence for antibiotic-induced endotoxin release? Infection 1998, 26, 77-84.
28. Mueller, M.; Lindner, B.; Kusumoto, S.; Fukase, K.; Schromm, A.B.; Seydel, U. Aggregates are the biologically active units of endotoxin. J. Biol. Chem. 2004, 279, 26307-26313.
29. Segawa, T.; Miyamoto, K.; Ushida, K.; Agata, K.; Okada, N.; Kohshima, S. Seasonal change in bacterial flora and biomass in mountain snow from the Tateyama Mountains, Japan, analyzed by 16S rRNA gene sequencing and real-time PCR. Appl. Environ. Microbiol. 2005, 71, 123-130.
30. Kaštovská, K.; Stibal, M.; Šabacká, M.; Černá, B.; Šantrůčková, H.; Elster, J. Microbial community structure and ecology of subglacial sediments in two polythermal Svalbard glaciers characterized by epifluorescence microscopy and PLFA. Polar Biol. 2007, 30, 277-287.
31. Mader, H.M.; Pettitt, M.E.; Wadham, J.L.; Wolff, E.W.; Parkes, R.J. Subsurface ice as a microbial habitat. Geology 2006, 34, 169-172.
32. Junge, K.; Krembs, C.; Deming, J.; Stierle, A.; Eicken, H. A microscopic approach to investigate bacteria under in situ conditions in sea-ice samples. Ann. Glaciol. 2001, 33, 304-310.
33. Tung, H.C.; Price, P.B.; Bramall, N.E.; Vrdoljak, G. Microorganisms metabolizing on clay grains in 3-km-deep Greenland basal ice. Astrobiology 2006, 6, 69-86.