Microorganisms in the accredit ice of Lake Vostok, Antarctica

Science

Volumn 286, Issue 5447, 1999, Pages 2144-2147

  Karl, D M ^a   Bird, D F ^b   Bjorkman K ^a   Houlihan, T ^a   Shackelford, R ^a   Tupas, L ^a  

^a UNIVERSITY OF HAWAII   (United States)

^b UNIVERSITÉ DU QUÉBEC À MONTRÉAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ICE; ACETIC ACID DERIVATIVE; ADENOSINE TRIPHOSPHATE; BACTERIAL PROTEIN; FRESH WATER; GLUCOSE; LIPOPOLYSACCHARIDE; NUCLEIC ACID;

BACTERIUM; ICE CORE; ICE THICKNESS; LACUSTRINE ENVIRONMENT; LAKE ECOSYSTEM; MICROORGANISM;

ANTARCTICA; ARTICLE; BACTERIUM IDENTIFICATION; GRAM NEGATIVE BACTERIUM; LAKE; MICROORGANISM; PRIORITY JOURNAL; BACTERIAL COUNT; BACTERIAL PHENOMENA AND FUNCTIONS; BACTERIUM; BIOMASS; ECOSYSTEM; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; OXIDATION REDUCTION REACTION; PHYSIOLOGY; SCANNING ELECTRON MICROSCOPY;

ANTARCTICA; LAKE VOSTOCK;

ACETATES; ADENOSINE TRIPHOSPHATE; ANTARCTIC REGIONS; BACTERIA; BACTERIAL PHYSIOLOGY; BACTERIAL PROTEINS; BIOMASS; COLONY COUNT, MICROBIAL; ECOSYSTEM; FLOW CYTOMETRY; FRESH WATER; GLUCOSE; GRAM-NEGATIVE BACTERIA; ICE; LIPOPOLYSACCHARIDES; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, FLUORESCENCE; NUCLEIC ACIDS; OXIDATION-REDUCTION; WATER MICROBIOLOGY;

EID: 0032589571     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5447.2144     Document Type: Article

References (33)

1. G. K. A. Oswald and G. de Q. Robin, Nature 245, 251 (1973); A. P. Kapitsa, J. K. Ridley, G. de Q. Robin, M. J. Siegert, I. A. Zotikov, Nature 381, 684 (1996)
2. G. K. A. Oswald and G. de Q. Robin, Nature 245, 251 (1973); A. P. Kapitsa, J. K. Ridley, G. de Q. Robin, M. J. Siegert, I. A. Zotikov, Nature 381, 684 (1996)
3. M. J. Siegert, J. A. Dowdeswell, M. R. Gorman, N. F. McIntyre, Antarct. Sci. 8, 281 (1996).
4. J. R. Petit et al., Nature 399, 429 (1999).
5. S. S. Abyzov, I. N. Mitskevich, M. N. Poglazova, Microbiology 67, 451 (1998).
6. J. R. Petit, in Lake Vostok Workshop Final Report, R. E. Bell and D. M. Karl, Eds. (National Science Foundation, Washington, DC, 1999), pp. 16-17; J. Jouzel et al., Science 286, 2138 (1999)
7. J. R. Petit, in Lake Vostok Workshop Final Report, R. E. Bell and D. M. Karl, Eds. (National Science Foundation, Washington, DC, 1999), pp. 16-17; J. Jouzel et al., Science 286, 2138 (1999)
8. 2O (final concentration, 11% ice). This procedure yielded three separate ice samples: bottom rinse I, bottom rinse II, and bottom melt. Visual inspection of the partially ablated ice cores before final melting revealed an apparently homogeneous solid with no evidence of fissures, cracks, or other imperfections.
9. -12 M). Total LPS (dissolved plus particulate) concentrations were measured in undiluted samples with the use of the turbidimetric Limulus amoebocyte lysate (LAL) assay [S. W. Watson, T. J. Novitsky, H. L. Quinby, F. W. Valois, Appl. Environ. Microbiol. 33, 940 (1977)] (12) and a computer-assisted commercially available instrument (LAL-5000; Associates of Cape Cod). Gram-negative bacterial cell carbon was estimated by assuming that LPS ranged from 6 to 15%, by weight, of total cell carbon, which corresponds to a C:LPS conversion factor of 6.7 to 16.7 (11). The detection limit for this assay was 0.025 pg of LPS per milliliter, and internal endotoxin standard recovery was 42.3 ± 5.1%.
10. 14C incorporation into nucleic acid and protein fractions [D. M. Karl, Appl. Environ. Microbiol. 44, 891 (1982)].
11. 2]). TOC was measured by means of a high-temperature (800 °C) combustion oxidation method [J. Qian and K. Mopper, Anal. Chem. 68, 3090 (1996)] with an automated TOC analyzer (MQ Scientific model 1001). Potassium hydrogen pthalate was used as the standard. Certified reference materials were used to establish instrument blank (0 μM DOC sample) and to determine measurement accuracy. Internal standards were recovered quantitatively.
12. J. C. Priscu et al., Science 285, 2141 (1999).
13. M. Maeda and N. Taga, J. Appl. Bact. 47, 175 (1979).
14. S. W. Watson and J. E. Hobbie, in Native Aquatic Bacteria: Enumeration, Activity and Ecology, J. W. Costerton and R. R. Colwell, Eds. (American Society for Testing and Materials, Philadelphia, PA, 1979), pp. 82-88.
15. D. M. Karl and F. C. Dobbs, in Molecular Approaches to the Study of the Ocean, K. E. Cooksey, Ed. (Chapman & Hall, London, 1998), pp. 29-89.
16. D. M. Karl, Microbiol. Rev. 44, 739 (1980).
17. J. H. Sharp et al., Mar. Chem. 48, 91 (1995).
18. M. Legrand and P. Mayewski, Rev. Geophys. 35, 219 (1997).
19. M. C. Kennicutt II, T. Sowers, B. Lyons, J. R. Petit, in Lake Vostok Workshop Final Report, R. E. Bell and D. M. Karl, Eds. (National Science Foundation, Washington, DC, 1999), pp. 26-28.
20. H. W. Jannasch and C. O. Wirsen, BioScience 29, 592 (1979); D. M. Karl, in The Microbiology of Deep-Sea Hydrothermal Vents, D. M. Karl, Ed. (CRC Press, Boca Raton, FL, 1995), pp. 35-124.
21. H. W. Jannasch and C. O. Wirsen, BioScience 29, 592 (1979); D. M. Karl, in The Microbiology of Deep-Sea Hydrothermal Vents, D. M. Karl, Ed. (CRC Press, Boca Raton, FL, 1995), pp. 35-124.
22. I. Dalziel, in Lake Vostok Workshop Final Report, R. E. Bell and D. M. Karl, Eds. (National Science Foundation, Washington, DC, 1999), pp. 17-19.
23. S. W. Squyres, R. T. Reynolds, P. M. Cassen, S. J. Peale, Nature 301, 225 (1983); M. H Carr et al., Nature 391, 363 (1998); C. R. Chapman, Science 283, 338 (1999); T. B. McCord et al., Science 280, 1242 (1998).
24. S. W. Squyres, R. T. Reynolds, P. M. Cassen, S. J. Peale, Nature 301, 225 (1983); M. H Carr et al., Nature 391, 363 (1998); C. R. Chapman, Science 283, 338 (1999); T. B. McCord et al., Science 280, 1242 (1998).
25. S. W. Squyres, R. T. Reynolds, P. M. Cassen, S. J. Peale, Nature 301, 225 (1983); M. H Carr et al., Nature 391, 363 (1998); C. R. Chapman, Science 283, 338 (1999); T. B. McCord et al., Science 280, 1242 (1998).
26. S. W. Squyres, R. T. Reynolds, P. M. Cassen, S. J. Peale, Nature 301, 225 (1983); M. H Carr et al., Nature 391, 363 (1998); C. R. Chapman, Science 283, 338 (1999); T. B. McCord et al., Science 280, 1242 (1998).
27. B. C. Monger and M. R. Landry, Appl. Environ. Microbiol. 59, 905 (1993).
28. Habitat data were taken from the following sources. South Atlantic: (12). Aleutian Trench: O. Holm-Hansen, R. Hodson, F. Azam, in Analytical Applications of Bioluminescence and Chemiluminescence, E. W. Chappelle and G. I. Picciolo, Eds. (NASA SP-388, Washington, DC, 1975), pp. 75-87. North Pacific gyre: (13); R. P. Shackelford, thesis, University of Hawaii (1998); the Hawaii Ocean Time series online database (http://hahana.soest. hawaii.edu/hot/hot_jgofs.html). Galapagos Rift: D. M. Karl, C. O. Wirsen, H. W. Jannasch, Science 207, 1345 (1980). Subglacial Ross Sea: F. Azam et al., Science 203, 451 (1979) . Antarctic Dry Valley lakes: C. D. Takacs and J. C. Priscu, Microb. Ecol. 36, 239 (1998).
29. Habitat data were taken from the following sources. South Atlantic: (12). Aleutian Trench: O. Holm-Hansen, R. Hodson, F. Azam, in Analytical Applications of Bioluminescence and Chemiluminescence, E. W. Chappelle and G. I. Picciolo, Eds. (NASA SP-388, Washington, DC, 1975), pp. 75-87. North Pacific gyre: (13); R. P. Shackelford, thesis, University of Hawaii (1998); the Hawaii Ocean Time series online database (http://hahana.soest. hawaii.edu/hot/hot_jgofs.html). Galapagos Rift: D. M. Karl, C. O. Wirsen, H. W. Jannasch, Science 207, 1345 (1980). Subglacial Ross Sea: F. Azam et al., Science 203, 451 (1979) . Antarctic Dry Valley lakes: C. D. Takacs and J. C. Priscu, Microb. Ecol. 36, 239 (1998).
30. Habitat data were taken from the following sources. South Atlantic: (12). Aleutian Trench: O. Holm-Hansen, R. Hodson, F. Azam, in Analytical Applications of Bioluminescence and Chemiluminescence, E. W. Chappelle and G. I. Picciolo, Eds. (NASA SP-388, Washington, DC, 1975), pp. 75-87. North Pacific gyre: (13); R. P. Shackelford, thesis, University of Hawaii (1998); the Hawaii Ocean Time series online database (http://hahana.soest. hawaii.edu/hot/hot_jgofs.html). Galapagos Rift: D. M. Karl, C. O. Wirsen, H. W. Jannasch, Science 207, 1345 (1980). Subglacial Ross Sea: F. Azam et al., Science 203, 451 (1979) . Antarctic Dry Valley lakes: C. D. Takacs and J. C. Priscu, Microb. Ecol. 36, 239 (1998).
31. Habitat data were taken from the following sources. South Atlantic: (12). Aleutian Trench: O. Holm-Hansen, R. Hodson, F. Azam, in Analytical Applications of Bioluminescence and Chemiluminescence, E. W. Chappelle and G. I. Picciolo, Eds. (NASA SP-388, Washington, DC, 1975), pp. 75-87. North Pacific gyre: (13); R. P. Shackelford, thesis, University of Hawaii (1998); the Hawaii Ocean Time series online database (http://hahana.soest. hawaii.edu/hot/hot_jgofs.html). Galapagos Rift: D. M. Karl, C. O. Wirsen, H. W. Jannasch, Science 207, 1345 (1980). Subglacial Ross Sea: F. Azam et al., Science 203, 451 (1979) . Antarctic Dry Valley lakes: C. D. Takacs and J. C. Priscu, Microb. Ecol. 36, 239 (1998).
32. Habitat data were taken from the following sources. South Atlantic: (12). Aleutian Trench: O. Holm-Hansen, R. Hodson, F. Azam, in Analytical Applications of Bioluminescence and Chemiluminescence, E. W. Chappelle and G. I. Picciolo, Eds. (NASA SP-388, Washington, DC, 1975), pp. 75-87. North Pacific gyre: (13); R. P. Shackelford, thesis, University of Hawaii (1998); the Hawaii Ocean Time series online database (http://hahana.soest. hawaii.edu/hot/hot_jgofs.html). Galapagos Rift: D. M. Karl, C. O. Wirsen, H. W. Jannasch, Science 207, 1345 (1980). Subglacial Ross Sea: F. Azam et al., Science 203, 451 (1979) . Antarctic Dry Valley lakes: C. D. Takacs and J. C. Priscu, Microb. Ecol. 36, 239 (1998).
33. We thank R. Bell and J. Priscu for invaluable discussions of Lake Vostok; K. Selph for the flow cytometric analyses; H. Vali, H. Campbell, and M. Lagacé for electron microscopy; B. Bays for preparation of Fig. 1; and L. Lum for assistance in the preparation of this manuscript. Supported by NSF (grant number OPP96-32763). This is SOEST publication 4931.