Quantitative analysis of plasma membrane proteome using two-dimensional difference gel electrophoresis

Methods in Molecular Biology

Volumn 876, Issue , 2012, Pages 67-82

  Tang, Wenqiang ^a,b  

^a GEOPHYSICAL LABORATORY   (United States)

^b HEBEI NORMAL UNIVERSITY   (China)

Author keywords

2 D DIGE; Brassinosteroids; Plasma membrane; Protein prefractionation; Proteomics; Signal transduction

Indexed keywords

CELL SURFACE RECEPTOR; PROTEOME; VEGETABLE PROTEIN;

ARABIDOPSIS; ARTICLE; CELL MEMBRANE; CYTOLYSIS; PLANT SEED; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEOMICS; QUANTITATIVE ANALYSIS; REGULATORY MECHANISM; SIGNAL TRANSDUCTION; TWO DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS; METABOLISM; METHODOLOGY;

CELL MEMBRANE; PLANT PROTEINS; PROTEOME; PROTEOMICS; TWO-DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS;

EID: 84865823685     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-809-2_5     Document Type: Article

References (12)

1. TangW, Deng Z, Wang ZY (2010) Proteomics shed light on the brassinosteroid signaling mechanisms. Curr Opin Plant Biol 13:27-33
2. Kosako H, Nagano K (2011) Quantitative phosphoproteomics strategies for understanding protein kinase-mediated signal transduction pathways. Expert Rev Proteomics 8:81-94
3. Choudhary C, Mann M (2010) Decoding signaling networks by mass spectrometry based proteomics. Nat Rev Mol Cell Biol 6:427-439
4. Stasyk T, Huber LA (2004) Zooming in: fractionation strategies in proteomics. Proteomics 4:3704-3716
5. Tang W, Deng Z, Oses-Prieto JA, Suzuki N, Zhu S, Zhang X, Burlingame AL, Wang Z-Y (2008) Proteomics studies of brassinosteroid signal transduction using prefractionation and two-dimensional DIGE. Mol Cell Proteomics 7:728-738
6. O'Farrell PH (1975) High resolution twodimensional electrophoresis of proteins. J Biol Chem 250:4007-4021
7. Unlu M, Morgan ME, Minden JS (1997) Difference gel electrophoresis: a single gel method for detecting changes in protein extracts. Electrophoresis 18:2071-2077
8. Tonge R, Shaw J, Middleton B, Rowlinson R, Rayner S, Young J, Pognan F, Hawkins E, Currie I, Davison M (2001) Validation and development of fluorescence two-dimensional differential gel electrophoresis proteomics technology. Proteomics 1:377-396
9. Deng Z, Zhang X, Tang W, Oses-Prieto JA, Suzuki N, Gendron JA, Chen H, Guan S, Chalkley RJ, Kaye Peterman T, Burlingame AL, Wang Z-Y (2007) A proteomic study of brassinosteroid response in Arabidopsis. Mol Cell Proteomics 6:2058-2071
10. Tang W, Kim T-W, Oses-Prieto JA, Sun Y, Deng Z, Zhu S, Wang R, Burlingame AL, Wang Z-Y (2008) BSKs mediate signal transduction from the receptor kinase BRI1 in Arabidopsis. Science 321:557-560
11. Widell S, Larsson C (1987) Plasma membrane purification. In: Senger H (ed) Blue light response: phenomena and occurrence in plants and microorganisms, vol II. CRC Press, Florida
12. Larsson C, Widell S, Kjellbom P (1987) Preparation of high-purity plasma membranes. Methods Enzymol 148:558-568