Extraction of proteins from gels: A brief review

Methods in Molecular Biology

Volumn 869, Issue , 2012, Pages 403-405

  Kurien, Biji T ^a   Scofield, R Hal ^a  

^a UNIVERSITY OF OKLAHOMA HEALTH SCIENCES CENTER   (United States)

Author keywords

Electroelution; Elution; Passive diffusion; Renaturation; SDS PAGE

Indexed keywords

ANTIGEN; POLYPEPTIDE; PROTEIN; TRYPSIN; ACETONE;

AMINO ACID ANALYSIS; AMINO ACID COMPOSITION; ANTIBODY PRODUCTION; ARTICLE; DIFFUSION; DISSOLUTION; ENZYME ACTIVITY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN LOCALIZATION; CHEMISTRY; ISOLATION AND PURIFICATION; METHODOLOGY; PRECIPITATION; REVIEW;

ACETONE; CHEMICAL PRECIPITATION; DIFFUSION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; PROTEINS;

EID: 84865813854     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-821-4_33     Document Type: Article

References (7)

1. Seelert H, Krause F (2008) Preparative isolation of protein complexes and other bioparticles by elution from polyacrylamide gels. Electrophoresis 29:2617-2636, Review
2. Ressler N (1958) Application of direct photographic photometry to preparative electrophoresis. Nature 182:463-464
3. Burgess RR (2009) Elution of proteins from gels. Methods Enzymol 463:565-572, Review
4. Lewis UJ, Clark MO (1963) Preparative methods for disk electrophoresis with special reference to the isolation of pituitary hormones. Anal Biochem 6:303-315
5. Jacobs E, Clad A (1986) Electroelution offixed and stained membrane proteins from preparative sodium dodecyl sulfate-polyacrylamide gels into a membrane trap. Anal Biochem 154:583-589
6. Shoji M, Kato M, Hashizume S (1995) Electrophoretic recovery of proteins from polyacrylamide gel. J Chromatogr A 698:145-162
7. May LT, Santhanam U, Sehgal PB (1991) On the multimeric nature of natural human interleukin-6. J Biol Chem 266:9950-9955