Production of rare ginsenosides (compound Mc, compound Y and aglycon protopanaxadiol) by β-glucosidase from Dictyoglomus turgidum that hydrolyzes β-linked, but not α-linked, sugars in ginsenosides

Biotechnology Letters

Volumn 34, Issue 9, 2012, Pages 1679-1686

  Lee, Gi Woong ^a   Kim, Kyoung Rok ^a   Oh, Deok Kun ^a  

^a Konkuk University   (South Korea)

Author keywords

Glucosidase; Aglycon protopanaxadiol; Compound Mc; Compound Y; Dictyoglomus turgidum; Ginsenoside

Indexed keywords

AGLYCON PROTOPANAXADIOL; COMPOUND MC; DICTYOGLOMUS TURGIDUM; GINSENOSIDES; GLUCOSIDASE;

ENZYMES; RUBIDIUM; SUGARS;

PHARMACODYNAMICS;

BETA GLUCOSIDASE; GINSENOSIDE; PROTOPANAXADIOL; SAPOGENIN;

ARTICLE; BACTERIUM; BIOTRANSFORMATION; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PH; TEMPERATURE; TIME;

BACTERIA; BETA-GLUCOSIDASE; BIOTRANSFORMATION; ENZYME STABILITY; GINSENOSIDES; HYDROGEN-ION CONCENTRATION; SAPOGENINS; TEMPERATURE; TIME FACTORS;

DICTYOGLOMUS;

EID: 84865802607     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-012-0949-9     Document Type: Article

References (17)

1. 1 into the rare gypenosides XVII and LXXV. Appl Environ Microbiol 76: 5827-5836.
2. c by human intestinal bacteria and its related antiallergic activity. Biol Pharm Bull 25: 743-747.
3. Bruce LZ, Henrik KN, Robert LS (1991) Thermostable enzymes for industrial applications. J Ind Microbiol Biotechnol 8: 71-81.
4. Chae S, Kang KA, Chang WY, Kim MJ, Lee SJ, Lee YS, Kim HS, Kim DH, Hyun JW (2009) Effect of compound K, a metabolite of ginseng saponin, combined with gamma-ray radiation in human lung cancer cells in vitro and in vivo. J Agric Food Chem 57: 5777-5782.
5. 1 by Acremonium strictum. Appl Microbiol Biotechnol 77: 1345-1350.
6. 2 and Rc from Panax ginseng by food microorganisms. Biol Pharm Bull 28: 2102-2105.
7. Cho WC, Chung WS, Lee SK, Leung AW, Cheng CH, Yue KK (2006) Ginsenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats. Eur J Pharmacol 550: 173-179.
8. Han Y, Sun B, Hu X, Zhang H, Jiang B, Spranger MI, Zhao Y (2007) Transformation of bioactive compounds by Fusarium sacchari fungus isolated from the soil-cultivated ginseng. J Agric Food Chem 55: 9373-9379.
9. Huang C, Wang G, Li H, Xie H, Sun J, Lv H, Lv T (2006) Sensitive and selective liquid chromatography-electrospray ionisation-mass spectrometry analysis of astragaloside-IV in rat plasma. J Pharm Biomed Anal 40: 788-793.
10. Noh KH, Oh DK (2009) Production of the rare ginsenosides compound K, compound Y, and compound Mc by a thermostable beta-glycosidase from Sulfolobus acidocaldarius. Biol Pharm Bull 32: 1830-1835.
11. Noh KH, Son JW, Kim HJ, Oh DK (2009) Ginsenoside compound K production from ginseng root extract by a thermostable beta-glycosidase from Sulfolobus solfataricus. Biosci Biotechnol Biochem 73: 316-321.
12. Park AR, Kim HJ, Lee JK, Oh DK (2010a) Hydrolysis and transglycosylation activity of a thermostable recombinant beta-glycosidase from Sulfolobus acidocaldarius. Appl Biochem Biotechnol 160: 2236-2247.
13. Park CS, Yoo MH, Noh KH, Oh DK (2010b) Biotransformation of ginsenosides by hydrolyzing the sugar moieties of ginsenosides using microbial glycosidases. Appl Microbiol Biotechnol 87: 9-19.
14. 1 by beta-glucosidase from Thermus caldophilus. Biotechnol Lett 30: 713-716.
15. 2, Rc and Rd by novel beta-glucosidase hydrolyzing outer 3-O-glycoside from Sphingomonas sp. 2F2: cloning, expression, and enzyme characterization. J Biotechnol 156: 125-133.
16. Yoo MH, Yeom SJ, Park CS, Lee KW, Oh DK (2011) Production of aglycon protopanaxadiol via compound K by a thermostable beta-glycosidase from Pyrococcus furiosus. Appl Microbiol Biotechnol 89: 1019-1028.
17. Yoshikawa M, Morikawa T, Kashima Y, Ninomiya K, Matsuda H (2003) Structures of new dammarane-type triterpene saponins from the flower buds of Panax notoginseng and hepatoprotective effects of principal ginseng saponins. J Nat Prod 66: 922-927.