Immunodetection of the recombinant GroEL by the Nanobody NbBruc02

World Journal of Microbiology and Biotechnology

Volumn 28, Issue 10, 2012, Pages 2987-2995

  Abo Assali, Lubna ^a   Al Mariri, Ayman ^b   Hamad, Ebtisam ^a   Abbady, Abdul Qader ^b  

^a DAMASCUS UNIVERSITY   (Syrian Arab Republic)

^b DEPARTMENT OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY   (Syrian Arab Republic)

Author keywords

Brucella; GroEL; Nanobody; Yersinia

Indexed keywords

3D STRUCTURE; BRUCELLA; CONFORMATIONAL CHANGE; CONFORMATIONAL EPITOPES; GROEL; HEAT SHOCK PROTEIN; HEAVY-CHAIN ANTIBODY FRAGMENTS; IMMUNOBLOTTING; IMMUNOCHROMATOGRAPHY; IMMUNODETECTION; NANOBODY; NANOMOLAR AFFINITY; PARASITE-; PROTEOMIC APPROACHES; STRONG INTERACTION; SURFACE PLASMON RESONANCE BIOSENSOR; YERSINIA;

BIOSENSORS; GENETIC ENGINEERING; SURFACE PLASMON RESONANCE;

ANTIGENS;

BACTERIAL ANTIGEN; CHAPERONIN 60; EPITOPE; IMMUNOGLOBULIN HEAVY CHAIN; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BRUCELLA; CAMEL; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOBLOTTING; IMMUNOLOGY; ISOLATION AND PURIFICATION; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; PROTEOMICS; SYRIAN ARAB REPUBLIC; YERSINIA;

ANIMALS; ANTIGENS, BACTERIAL; BRUCELLA; CAMELS; CHAPERONIN 60; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; IMMUNOBLOTTING; IMMUNODOMINANT EPITOPES; IMMUNOGLOBULIN HEAVY CHAINS; PROTEIN CONFORMATION; PROTEOMICS; RECOMBINANT PROTEINS; SYRIA; YERSINIA;

BRUCELLA; YERSINIA;

EID: 84865793861     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-012-1109-z     Document Type: Article

References (39)

1. Abbady AQ, Al-Mariri A, Zarkawi M, Al-Assad A, Muyldermans S (2011) Evaluation of a nanobody phage display library constructed from a Brucella-immunised camel. Vet Immunol Immunopathol 142(1-2): 49-56.
2. Abbady AQ, Al-Daoude A, Al-Mariri A, Zarkawi M, Muyldermans S (2012) Chaperonin GroEL a Brucella immunodominant antigen identified using Nanobody and MALDI-TOF-MS technologies. Vet Immunol Immunopathol 146(3-4): 254-263.
3. Al Dahouk S, Nockler K, Scholz HC, Tomaso H, Bogumil R, Neubauer H (2006) Immunoproteomic characterization of Brucella abortus 1119-3 preparations used for the serodiagnosis of Brucella infections. J Immunol Methods 309(1-2): 34-47.
4. Alonso-Urmeneta B, Marin C, Aragon V, Blasco JM, Diaz R, Moriyon I (1998) Evaluation of lipopolysaccharides and polysaccharides of different epitopic structures in the indirect enzyme-linked immunosorbent assay for diagnosis of brucellosis in small ruminants and cattle. Clin Diagn Lab Immunol 5(6): 749-754.
5. Amirmozafari N, Ghazi F, Mostafazadeh A, Mostafaie A, Rajabnia R (2008) Comparison of heat shock response in Brucella abortus and Brucella melitensis. Pak J Biol Sci 11(2): 188-194.
6. Arbabi Ghahroudi M, Desmyter A, Wyns L, Hamers R, Muyldermans S (1997) Selection and identification of single domain antibody fragments from camel heavy-chain antibodies. FEBS Lett 414(3): 521-526.
7. Bae JE, Toth TE (2000) Cloning and kinetics of expression of Brucella abortus heat shock proteins by baculovirus recombinants. Vet Microbiol 75(2): 199-204.
8. Bae JE, Schurig GG, Toth TE (2002) Mice immune responses to Brucella abortus heat shock proteins. Use of baculovirus recombinant-expressing whole insect cells, purified Brucella abortus recombinant proteins, and a vaccinia virus recombinant as immunogens. Vet Microbiol 88(2): 189-202.
9. Baloglu S, Toth TE, Schurig GG, Sriranganathan N, Boyle SM (2000) Humoral immune response of BALB/c mice to a vaccinia virus recombinant expressing Brucella abortus GroEL does not correlate with protection against a B. abortus challenge. Vet Microbiol 76(2): 193-199.
10. Basharov MA (2003) Protein folding. J Cell Mol Med 7(3): 223-237.
11. Blanco-Toribio A, Muyldermans S, Frankel G, Fernandez LA (2010) Direct injection of functional single-domain antibodies from E. coli into human cells. PLoS One 5(12): e15227.
12. Castaneda-Roldan EI, Ouahrani-Bettache S, Saldana Z, Avelino F, Rendon MA, Dornand J, Giron JA (2006) Characterization of SP41, a surface protein of Brucella associated with adherence and invasion of host epithelial cells. Cell Microbiol 8(12): 1877-1887.
13. Chuang VT, Maruyama T, Otagiri M (2009) Updates on contemporary protein binding techniques. Drug Metab Pharmacokinet 24(4): 358-364.
14. Connolly JP, Comerci D, Alefantis TG, Walz A, Quan M, Chafin R, Grewal P, Mujer CV, Ugalde RA, DelVecchio VG (2006) Proteomic analysis of Brucella abortus cell envelope and identification of immunogenic candidate proteins for vaccine development. Proteomics 6(13): 3767-3780.
15. Habicht G, Haupt C, Friedrich RP, Hortschansky P, Sachse C, Meinhardt J, Wieligmann K, Gellermann GP, Brodhun M, Gotz J, Halbhuber KJ, Rocken C, Horn U, Fandrich M (2007) Directed selection of a conformational antibody domain that prevents mature amyloid fibril formation by stabilizing Abeta protofibrils. Proc Natl Acad Sci U S A 104(49): 19232-19237.
16. Hendrickx ML, De Winter A, Buelens K, Compernolle G, Hassanzadeh-Ghassabeh G, Muyldermans S, Gils A, Declerck PJ (2011) TAFIa inhibiting nanobodies as profibrinolytic tools and discovery of a new TAFIa conformation. J Thromb Haemost 9(11): 2268-2277.
17. Kaufmann SH, Schoel B, van Embden JD, Koga T, Wand-Wurttenberger A, Munk ME, Steinhoff U (1991) Heat-shock protein 60: implications for pathogenesis of and protection against bacterial infections. Immunol Rev 121: 67-90.
18. Kirchhofer A, Helma J, Schmidthals K, Frauer C, Cui S, Karcher A, Pellis M, Muyldermans S, Casas-Delucchi CS, Cardoso MC, Leonhardt H, Hopfner KP, Rothbauer U (2010) Modulation of protein properties in living cells using nanobodies. Nat Struct Mol Biol 17(1): 133-138.
19. Kuroda K, Kato M, Mima J, Ueda M (2006) Systems for the detection and analysis of protein-protein interactions. Appl Microbiol Biotechnol 71(2): 127-136.
20. Lin J, Ficht TA (1995) Protein synthesis in Brucella abortus induced during macrophage infection. Infect Immun 63(4): 1409-1414.
21. Lin Z, Rye HS (2006) GroEL-mediated protein folding: making the impossible, possible. Crit Rev Biochem Mol Biol 41(4): 211-239.
22. Lin J, Adams LG, Ficht TA (1992) Characterization of the heat shock response in Brucella abortus and isolation of the genes encoding the GroE heat shock proteins. Infect Immun 60(6): 2425-2431.
23. Lund PA (2009) Multiple chaperonins in bacteria-why so many? FEMS Microbiol Rev 33(4): 785-800.
24. Oliveira SC, Harms JS, Banai M, Splitter GA (1996) Recombinant Brucella abortus proteins that induce proliferation and gamma-interferon secretion by CD4 + T cells from Brucella-vaccinated mice and delayed-type hypersensitivity in sensitized guinea pigs. Cell Immunol 172(2): 262-268.
25. Omidfar K, Rasaee MJ, Modjtahedi H, Forouzandeh M, Taghikhani M, Golmakani N (2004) Production of a novel camel single-domain antibody specific for the type III mutant EGFR. Tumor Biol 25(5-6): 296-305.
26. Pappas G, Akritidis N, Bosilkovski M, Tsianos E (2005) Brucellosis. N Engl J Med 352(22): 2325-2336.
27. Pappas G, Panagopoulou P, Christou L, Akritidis N (2006) Brucella as a biological weapon. Cell Mol Life Sci 63(19-20): 2229-2236.
28. Retzlaff C, Yamamoto Y, Hoffman PS, Friedman H, Klein TW (1994) Bacterial heat shock proteins directly induce cytokine mRNA and interleukin-1 secretion in macrophage cultures. Infect Immun 62(12): 5689-5693.
29. Rothbauer U, Zolghadr K, Muyldermans S, Schepers A, Cardoso MC, Leonhardt H (2008) A versatile nanotrap for biochemical and functional studies with fluorescent fusion proteins. Mol Cell Proteomics 7(2): 282-289.
30. Saerens D, Kinne J, Bosmans E, Wernery U, Muyldermans S, Conrath K (2004) Single domain antibodies derived from dromedary lymph node and peripheral blood lymphocytes sensing conformational variants of prostate-specific antigen. J Biol Chem 279(50): 51965-51972.
31. Sambrook J, Russel DW (2001) Molecular cloning: a laboratory manual, vol 3rd ed. Cold Spring Harbour, New York.
32. Serruys B, van Houtte F, Verbrugghe P, Leroux-Roels G, Vanlandschoot P (2009) Llama-derived single-domain intrabodies inhibit secretion of hepatitis B virions in mice. Hepatology 49(1): 39-49.
33. Skerra A, Pluckthun A (1988) Assembly of a functional immunoglobulin Fv fragment in Escherichia coli. Science 240(4855): 1038-1041.
34. Stevens MG, Olsen SC, Pugh GW, Mayfield JE (1997) Role of immune responses to a GroEL heat shock protein in preventing brucellosis in mice vaccinated with Brucella abortus strain RB51. Comp Immunol Microbiol Infect Dis 20(2): 147-153.
35. Stijlemans B, Caljon G, Natesan SK, Saerens D, Conrath K, Perez-Morga D, Skepper JN, Nikolaou A, Brys L, Pays E, Magez S, Field MC, De Baetselier P, Muyldermans S (2011) High affinity nanobodies against the Trypanosome brucei VSG are potent trypanolytic agents that block endocytosis. PLoS Pathog 7(6): e1002072.
36. Teixeira-Gomes AP, Cloeckaert A, Bezard G, Bowden RA, Dubray G, Zygmunt MS (1997a) Identification and characterization of Brucella ovis immunogenic proteins using two-dimensional electrophoresis and immunoblotting. Electrophoresis 18(8): 1491-1497.
37. Teixeira-Gomes AP, Cloeckaert A, Bezard G, Dubray G, Zygmunt MS (1997b) Mapping and identification of Brucella melitensis proteins by two-dimensional electrophoresis and microsequencing. Electrophoresis 18(1): 156-162.
38. Watarai M, Kim S, Erdenebaatar J, Makino S, Horiuchi M, Shirahata T, Sakaguchi S, Katamine S (2003) Cellular prion protein promotes Brucella infection into macrophages. J Exp Med 198(1): 5-17.
39. Zarebski LM, Urrutia M, Goldbaum FA (2005) Llama single domain antibodies as a tool for molecular mimicry. J Mol Biol 349(4): 814-824.