Assessment of technical protocols for novel embryonic stem cell tests with molecular markers (Hand1- and Cmya1-ESTs): A preliminary cross-laboratory performance analysis

Journal of Toxicological Sciences

Volumn 37, Issue 4, 2012, Pages 845-851

  Suzuki, Noriyuki ^a   Yamashita, Norihisa ^a   Koseki, Naoteru ^b   Yamada, Toru ^b   Kimura, Yutaka ^c   Aiba, Setsuya ^c   Toyoizumi, Tomoyasu ^d   Watanabe, Mika ^d   Ohta, Ryo ^d   Tanaka, Noriho ^d   Saito, Koichi ^a  

^a SUMITOMO CHEMICAL CO LTD   (Japan)

^b DAINIPPON SUMITOMO PHARMA CO LTD   (Japan)

^c TOHOKU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^d HATANO RESEARCH INSTITUTE   (Japan)

Author keywords

Differentiation; Embryonic stem cell test; Embryonic stem cells; Embryotoxicity; In vitro; Reporter gene assay

Indexed keywords

6 AMINONICOTINAMIDE; ASCORBIC ACID; CARDIOMYOPATHY ASSOCIATED 1; HEART AND NEURAL CREST DERIVATIVES EXPRESSED TRANSCRIPT 1; HYDROXYUREA; MOLECULAR MARKER; PENICILLIN G SODIUM; UNCLASSIFIED DRUG; VALPROIC ACID;

ANIMAL CELL; ASSAY; CLINICAL ASSESSMENT; CONTROLLED STUDY; CYTOTOXICITY TEST; EMBRYONIC STEM CELL TEST; EMBRYOTOXICITY; LABORATORY TEST; LETTER; MEDICAL DEVICE; METHODOLOGY; MOUSE; NONHUMAN; REPORTER GENE; TOXICITY TESTING;

EID: 84865654848     PISSN: 03881350     EISSN: 18803989     Source Type: Journal    
DOI: 10.2131/jts.37.845     Document Type: Letter

References (17)

1. Bremer, S. and Hartung, T. (2004): The use of embryonic stem cells for regulatory developmental toxicity testing in vitro - the current status of test development. Curr. Pharm. Des., 10, 2733-2747.
2. Brown, N.A. (2002): Selection of test chemicals for the ECVAM international validation study on in vitro embryotoxicity tests, ATLA, 30, 177-198.
3. Buesen, R., Genschow, E., Slawik, B., Visan, A., Spielmann, H., Luch, A. and Seiler, A. (2009): Embryonic stem cell test remastered: comparison between the validated EST and the new molecular FACS-EST for assessing developmental toxicity in vitro. Toxicol. Sci., 108, 389-400.
4. Chen, R., Chen, J., Cheng, S., Qin, J., Li, W., Zhang, L., Jiao, H., Yu, X., Zhang, X., Lahn, B.T. and Xiang, A.P. (2010): Assessment of embryotoxicity of compounds in cosmetics by the embryonic stem cell test. Toxicol. Mech. Methods, 20, 112-118.
5. de Jong, E., Louisse, J., Verwei, M., Blaauboer, B.J., van de Sandt, J.J., Woutersen, R.A., Rietjens, I.M. and Piersma, A.H. (2009): Relative developmental toxicity of glycol ether alkoxy acid metabolites in the embryonic stem cell test as compared with the in vivo potency of their parent compounds. Toxicol. Sci., 110, 117-124.
6. di Guglielmo, C., Lopez, D.R., De Lapuente, J., Mallafre, J.M. and Suarez, M.B. (2010): Embryotoxicity of cobalt ferrite and gold nanoparticles: a first in vitro approach. Reprod. Toxicol., 30, 271-276.
7. Eckardt, K. and Stahlmann, R. (2010): Use of two validated in vitro tests to assess the embryotoxic potential of mycophenolic acid. Arch. Toxicol., 84, 37-43.
8. Festag, M., Viertel, B., Steinberg, P. and Sehner, C. (2007): An in vitro embryotoxicity assay based on the disturbance of the differ- entiation of murine embryonic stem cells into endothelial cells. II. Testing of compounds. Toxicol. In Vitro, 21, 1631-1640.
9. Genschow, E., Spielmann, H., Scholz, G., Pohl, I., Seiler, A., Clemann, N., Bremer, S. and Becker, K. (2004): Validation of the embryonic stem cell test in the international ECVAM validation study on three in vitro embryotoxicity tests. Altern. Lab. Anim. 32, 209-244.
10. Paquette, J.A., Kumpf, S.W., Streck, R.D., Thomson, J.J., Chapin, R.E. and Stedman, D.B. (2008): Assessment of the Embryonic Stem Cell Test and application and use in the pharmaceutical industry. Birth Defects Res. B Dev. Reprod. Toxicol., 83, 104-111.
11. Pellizzer, C., Adler, S., Corvi, R., Hartung, T. and Bremer, S. (2004): Monitoring of teratogenic effects in vitro by analysing a selected gene expression pattern. Toxicol. In Vitro, 18, 325-335.
12. Scholz, G., Pohl, I., Genschow, E., Klemm, M. and Spielmann, H. (1999): Embryotoxicity screening using embryonic stem cells in vitro: Correlation to in vivo teratogenicity. Cells Tissues Organs, 165, 203-211.
13. Spielmann, H., Pohl, I., Doring, B., Liebsch, M. and Moldenhauer, F. (1997): The embryonic stem cell test (EST), an in vitro embryotoxicity test using two permanent mouse cell lines: 3T3 fibroblasts and embryonic stem cells. Toxicol. In Vitro, 10, 119-127.
14. Spielmann, H. and Vogel, R. (2006): REACH testing requirements must not be driven by reproductive toxicity testing. Altern. Lab. Anim., 34, 365-366.
15. Suzuki, N., Ando, S., Horie, N., Sumida, K. and Saito, K. (2011a): Analysis of altered gene expression specific to embryotoxic chemical treatment during embryonic stem cell differentiation into myocardiac and neural cells. J. Toxicol. Sci., 36, 569-585.
16. Suzuki, N., Ando, S., Yamashita, N., Horie, N. and Saito, K. (2011b): Evaluation of novel high-throughput embryonic stem cell tests with new molecular markers for screening embryotoxic chemicals in vitro. Toxicol. Sci., 124, 460-471.
17. zur Nieden, N.I., Kempka, G., and Ahr, H.J. (2004): Molecular multiple endpoint embryonic stem cell test - A possible approach to test for the teratogenic potential of compounds. Toxicol. Appl. Pharmacol., 194, 257-269.