Detection of apoptosis by TUNEL assay

Methods in Molecular Biology

Volumn 887, Issue , 2012, Pages 41-47

  Kyrylkova, Kateryna ^a   Kyryachenko, Sergiy ^a   Leid, Mark ^a   Kioussi, Chrissa ^a  

^a OREGON STATE UNIVERSITY   (United States)

Author keywords

Apoptosis; Frozen sections; Immunofluorescence; Mouse embryos; TUNEL

Indexed keywords

DNA NUCLEOTIDYLEXOTRANSFERASE;

APOPTOSIS; ARTICLE; DNA DEGRADATION; DNA TEMPLATE; DOUBLE STRANDED DNA BREAK; FROZEN SECTION; HISTOLOGY; NICK END LABELING; NONHUMAN; PRIORITY JOURNAL; ROOM TEMPERATURE; TOOTH DEVELOPMENT; ANIMAL; ANIMAL EMBRYO; CYTOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; METHODOLOGY; MOUSE; PHYSIOLOGY;

ANIMALS; APOPTOSIS; EMBRYO, MAMMALIAN; FLUORESCENT ANTIBODY TECHNIQUE; FROZEN SECTIONS; IN SITU NICK-END LABELING; MICE;

EID: 84865591708     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-860-3_5     Document Type: Article

References (11)

1. Saraste A. (1999) Morphologic criteria and detection of apoptosis. Herz 24 (3), 189-195.
2. Saraste A., Pulkki K. (2000) Morphologic and biochemical hallmarks of apoptosis. Cardiovasc Res 45 (3), 528-537.
3. Cohen J. J. (1993) Apoptosis. Immunol Today 14 (3), 126-130.
4. Bortner C. D., Oldenburg N. B., Cidlowski J. A. (1995) The role of DNA fragmentation in apoptosis. Trends Cell Biol 5 (1), 21-26.
5. Gavrieli Y., Sherman Y., Ben-Sasson S. A. (1992) Identi fi cation of programmed cell death in situ via speci fi c labeling of nuclear DNA fragmentation. J Cell Biol 119 (3), 493-501.
6. Nagoescu A., Lorimier P., Labat-Moleur F., Drouet C., Robert C., Guillermet C., Brambilla C., Brambilla E. (1996) In situ apoptotic cell labeling by the TUNEL method: improvement and evaluation on cell preparations. J Histochem Cytochem 44 (9), 959-968.
7. Rode H. D., Eisel D., Frost I. (2004) Apoptosis, cell death and cell proliferation. 3 rd ed. London: Roche Applied Science.
8. Grasl-Kraupp B., Ruttkay-Nedecky B., Koudelka H. Bukowska K., Bursch W., Schulte- Hermann R. (1995) In situ detection of fragmented DNA (TUNEL assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: a cautionary note. Hepatology 21 (5), 1465-1468.
9. Orita Y., Nishizaki K., Sasaki J., Kanda S., Kimura N., Nomiya S., Yuen K., Masuda Y. (1999) Does TUNEL staining during peri- and post-natal development of the mouse inner ear indicate apoptosis? Acta Otolaryngol Suppl 540, 22-26.
10. Tornusciolo D. R., Schmidt R. E., Roth K. A. (1995) Simultaneous detection of TDTmediated dUTP-biotin nick end-labeling (TUNEL)-positive cells and multiple immunohistochemical markers in single tissue sections. Biotechniques 19 (5), 800-805.
11. Oberhaus S. M. (2003) TUNEL and immuno fl uorescence double-labeling assay for apoptotic cells with speci fi c antigen(s). Methods Mol Biol 218, 85-96.