Comparison of rapid methods for detection of Giardia spp. and Cryptosporidium spp. (Oo)cysts using transportable instrumentation in a field deployment

Environmental Science and Technology

Volumn 46, Issue 16, 2012, Pages 8952-8959

  Keserue, Hans Anton ^a,b,c   Füchslin, Hans Peter ^a,d   Wittwer, Matthias ^e   Nguyen Viet, Hung ^a,f,g   Nguyen, Thuy Tram ^h   Surinkul, Narong ⁱ   Koottatep, Thammarat ⁱ   Schürch, Nadia ^e   Egli, Thomas ^a,c  

^a SWISS FEDERAL INSTITUTE OF AQUATIC SCIENCE AND TECHNOLOGY   (Switzerland)

^b FEDERAL OFFICE OF PUBLIC HEALTH   (Switzerland)

^c ETH ZURICH   (Switzerland)

^d Bachema AG   (Switzerland)

^e SPIEZ LABORATORY   (Switzerland)

^f SWISS TROPICAL AND PUBLIC HEALTH INSTITUTE   (Switzerland)

^g HANOI UNIVERSITY OF PUBLIC HEALTH   (Viet Nam)

^h NATIONAL INSTITUTE OF HYGIENE AND EPIDEMIOLOGY   (Viet Nam)

ⁱ ASIAN INSTITUTE OF TECHNOLOGY   (Thailand)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYSIS TIME; CANAL WATER; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; CRYPTOSPORIDIUM SPP; DISASTER MANAGEMENT; FIELD CAMPAIGN; FIELD DEPLOYMENT; FIELD STUDIES; GIARDIA; GIARDIA LAMBLIA; IMMUNOMAGNETIC SEPARATION; IRRIGATION WATERS; MOBILE INSTRUMENTATION; PATHOGENIC ORGANISMS; PCR REACTIONS; QUANTITATIVE DETECTION; RAPID DETECTION; RAPID METHOD; RAPID SCREENING; THAILAND;

DISASTER PREVENTION; FLOW CYTOMETRY; IRRIGATION; PROTOZOA; VEGETABLES; WATER ANALYSIS; WATER SUPPLY;

POLYMERASE CHAIN REACTION;

COMPARATIVE STUDY; CONCENTRATION (COMPOSITION); CYST; DETECTION METHOD; EUKARYOTE; FLOW CYTOMETRY; INSTRUMENTATION; POLYMERASE CHAIN REACTION;

ARTICLE; BACTERIAL CELL; CELL COUNT; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; DIPLOMONADIDA; ESCHERICHIA COLI; FILTRATION; FLOW CYTOMETRY; GIARDIA LAMBLIA; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOMAGNETIC SEPARATION; INTERMETHOD COMPARISON; MICROORGANISM DETECTION; NONHUMAN; OOCYST; PLATE COUNT; POLYMERASE CHAIN REACTION; PURIFICATION; QUANTITATIVE ANALYSIS; RELIABILITY; VEGETABLE; WASTE WATER; WATER ANALYSIS; WATER CONTAMINATION;

ANIMALS; CRYPTOSPORIDIUM; FLOW CYTOMETRY; GIARDIA; MICROSCOPY, FLUORESCENCE; OOCYSTS; POLYMERASE CHAIN REACTION;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; GIARDIA; GIARDIA INTESTINALIS;

EID: 84865499158     PISSN: 0013936X     EISSN: 15205851     Source Type: Journal    
DOI: 10.1021/es301974m     Document Type: Article

References (42)

1. Wampler, P. Pick sanitation over vaccination in Haiti Nature 2011, 470, 175
2. Webster, P. C. Lack of clean water exacerbates cholera outbreak in Haiti CMAJ 2011, 183, E83-E84
3. McCoy, W. F.; Olson, B. H. Relationship among turbidity, particle counts and bacteriological quality within water distribution lines Water Res. 1986, 20, 1023-1029
4. Bej, A. K.; McCarty, S. C.; Atlas, R. M. Detection of coliform bacteria and Escherichia coli by multiplex polymerase chain reaction: Comparison with defined substrate and plating methods for water quality monitoring Appl. Environ. Microbiol. 1991, 57, 2429-2432
5. Allen, M. J.; Edberg, S. C.; Reasoner, D. J. Heterotrophic plate count bacteria-What is their significance in drinking water? Int. J. Food Microbiol. 2004, 92, 265-274 (Pubitemid 38625562)
6. Reasoner, D. J. Heterotrophic plate count methodology in the United States Int. J. Food Microbiol. 2004, 92, 307-315 (Pubitemid 38625566)
7. Diamond, L. S.; Harlow, D. R.; Cunnick, C. C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba Trans. R. Soc. Trop. Med. Hyg. 1978, 72, 431-432 (Pubitemid 8389164)
8. Rice, E. W.; Schaefer, F. W. Improved in vitro excystation procedure for Giardia lamblia cysts J. Clin. Microbiol. 1981, 14, 709-710 (Pubitemid 12242083)
9. Robertson, L. J.; Campbell, A. T.; Smith, H. V. In vitro excystation of Cryptosporidium parvum Parasitology 1993, 106 (Pt 1) 13-19 (Pubitemid 23025271)
10. Keserue, H.-A.; Füchslin, H. P.; Egli, T. Rapid detection and enumeration of Giardia lamblia cysts in water samples by immunomagnetic separation and flow cytometric analysis Appl. Environ. Microbiol. 2011, 77, 5420-5427
11. Laberge, I.; Griffiths, M. W.; Griffiths, M. W. Prevalence, detection and control of Cryptosporidium parvum in food Int. J. Food Microbiol. 1996, 32, 1-26 (Pubitemid 26297254)
12. Karanis, P. Giardia and Cryptosporidium: Occurrence in water supplies. In Encyclopedia of Environmental Health; Elsevier: Burlington, 2011; pp 946-954.
13. Smith, H. V.; Cacciò, S. M.; Cook, N.; Nichols, R. A. B.; Tait, A. Cryptosporidium and Giardia as foodborne zoonoses Vet. Parasitol. 2007, 149, 29-40 (Pubitemid 47398932)
14. Smith, H. V.; Rose, J. B. Waterborne cryptosporidiosis Parasitol. Today 1990, 6, 8-12
15. Monis, P..; Thompson, R. C. Cryptosporidium and Giardia -zoonoses: Fact or fiction? Infect. Genet. Evol. 2003, 3, 233-244 (Pubitemid 37408274)
16. Marshall, M.; Naumovitz, D.; Ortega, Y.; Sterling, C. Waterborne protozoan pathogens Clin. Microbiol. Rev. 1997, 10, 67-85 (Pubitemid 27042832)
17. Rose, J. B.; Slifko, T. R. Giardia, Cryptosporidium, and Cyclospora and their impact on foods: A review J. Food Prot. 1999, 62, 1059-1070 (Pubitemid 29432335)
18. LeChevallier, M. W.; Norton, W. D.; Lee, R. G. Occurrence of Giardia and Cryptosporidium spp. in surface water supplies Appl. Environ. Microbiol. 1991, 57, 2610-2616
19. Smith, H. V.; Nichols, R. A. B. Cryptosporidium: Detection in water and food Exp. Parasitol. 2010, 124, 61-79
20. Smith, R.; Von Tress, M.; Tubb, C.; Vanhaecke, E. Evaluation of the ScanRDI as a rapid alternative to the pharmacopoeial sterility test method: Comparison of the limits of detection PDA J. Pharm. Sci. Technol. 2010, 64, 356-363
21. Rendtorff, R. C. The experimental transmission of Giardia lamblia among volunteer subjects. In Waterborne Transmission of Giardiasis; U.S. Environmental Protection Agency: Cincinnati, OH, 1978; pp 64-81.
22. Rendtorff, R. C. The experimental transmission of human intestinal protozoan parasites. II. Giardia lamblia cysts given in capsules Am. J. Hyg. 1954, 59, 209-220
23. Chappell, C. L.; Okhuysen, P. C.; DuPont, H. L.; Sterling, C. R.; Tzipori, S. Chapter 7-Cryptosporidium parvum volunteer study: Infectivity and immunity. In Cryptosporidium; Elsevier: Amsterdam, 2003; pp 79-81.
24. U.S. Environmental Protection Agency USEPA method 1623: Cryptosporidium and Giardia in water by filtration/IMS/FA. In; EPA 821-R-01-025; Office of Water, U. S. Environmental Protection Agency: Washington, D.C., 2005; Vol. EPA 821-R-01-025.
25. Guillot, E.; Loret, J. F. Waterborne Pathogens: Review for the Drinking Water Industry; IWA Publishing: London, United Kingdom, 2010.
26. Clancy, J. L.; Gollnitz, W. D.; Tabib, Z. Commercial labs: How accurate are they? J. Am. Water Works Assoc. 1994, 89-97
27. DiGiorgio, C. L.; Gonzalez, D. A.; Huitt, C. C. Cryptosporidium and Giardia recoveries in natural waters by using Environmental Protection Agency method 1623 Appl. Environ. Microbiol. 2002, 68, 5952-5955 (Pubitemid 36342427)
28. Goldstein, S. T.; Juranek, D. D.; Ravenholt, O.; Hightower, A. W.; Martin, D. G.; Mesnik, J. L.; Griffiths, S. D.; Bryant, A. J.; Reich, R. R.; Herwaldt, B. L. Cryptosporidiosis: An outbreak associated with drinking water despite state-of-the-art water treatment Ann. Intern. Med. 1996, 124, 459-468 (Pubitemid 26070110)
29. Mac Kenzie, W. R.; Schell, W. L.; Blair, K. A.; Addiss, D. G.; Peterson, D. E.; Hoxie, N. J.; Kazmierczak, J. J.; Davis, J. P. Massive outbreak of waterborne Cryptosporidium infection in Milwaukee, Wisconsin: recurrence of illness and risk of secondary transmission Clin. Infect. Dis. 1995, 21, 57-62
30. Anceno, A. J.; Ozaki, M.; Dang, Y. N. D.; Chuluun, B.; Shipin, O. V. Canal networks as extended waste stabilization ponds: Fate of pathogens in constructed waterways in Pathumthani Province, Thailand Water Sci. Technol. 2007, 55, 143-156 (Pubitemid 46957819)
31. Diallo, M. B. C.; Anceno, A. J.; Tawatsupa, B.; Houpt, E. R.; Wangsuphachart, V.; Shipin, O. V. Infection risk assessment of diarrhea-related pathogens in a tropical canal network Sci. Total Environ. 2008, 407, 223-232
32. Guy, R. A.; Payment, P.; Krull, U. J.; Horgen, P. A. Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage Appl. Environ. Microbiol. 2003, 69, 5178-5185 (Pubitemid 37150736)
33. Hammes, F.; Berney, M.; Wang, Y.; Vital, M.; Köster, O.; Egli, T. Flow-cytometric total bacterial cell counts as a descriptive microbiological parameter for drinking water treatment processes Water Res. 2008, 42, 269-277
34. APHA, AWWA, and WPCF 9215 Heterotrophic plate count. In Standard Methods for the Examination of Water and Wastewater; American Public Health Association: Washington, DC., USA., 2005.
35. APHA, AWWA, and WPCF 9221 F. Escherichia coli procedure. In Standard Methods for the Examination of Water and Wastewater; American Public Health Association: Washington, DC., USA., 2005.
36. Rochelle, P. A.; De Leon, R.; Stewart, M. H.; Wolfe, R. L. Comparison of primers and optimization of PCR conditions for detection of Cryptosporidium parvum and Giardia lamblia in water Appl. Environ. Microbiol. 1997, 63, 106-114 (Pubitemid 27020376)
37. McIntyre, L.; Hoang, L.; Ong, C. S. L.; Lee, P.; Isaac-Renton, J. L. Evaluation of molecular techniques to biotype Giardia duodenalis collected during an outbreak J. Parasitol. 2000, 86, 172-177 (Pubitemid 30126496)
38. Cacciò, S. M.; De Giacomo, M.; Aulicino, F. A.; Pozio, E. Giardia cysts in wastewater treatment plants in Italy Appl. Environ. Microbiol. 2003, 69, 3393-3398 (Pubitemid 36693346)
39. Campbell, A. T.; Robertson, L. J.; Smith, H. V. Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes Appl. Environ. Microbiol. 1992, 58, 3488-3493
40. Krometis, L.-A. H.; Characklis, G. W.; Sobsey, M. D. Identification of particle size classes inhibiting protozoan recovery from surface water samples via U.S. Environmental Protection Agency Method 1623 Appl. Environ. Microbiol. 2009, 75, 6619-6621
41. Füchslin, H. P.; Kötzsch, S.; Keserue, H.-A.; Egli, T. Rapid and quantitative detection of Legionella pneumophila applying immunomagnetic separation and flow cytometry Cytometry 2010, 77A, 264-274
42. Feng, Y.; Zhao, X.; Chen, J.; Jin, W.; Zhou, X.; Li, N.; Wang, L.; Xiao, L. Occurrence, source, and human infection potential of Cryptosporidium and Giardia spp. in source and tap water in Shanghai, China Appl. Environ. Microbiol. 2011, 77, 3609-3616