Isolation of cell nuclei in microchannels by short-term chemical treatment via two-step carrier medium exchange

Biomedical Microdevices

Volumn 14, Issue 4, 2012, Pages 751-757

  Toyama, Kaori ^a   Yamada, Masumi ^a   Seki, Minoru ^a  

^a CHIBA UNIVERSITY   (Japan)

Author keywords

Carrier medium exchange; Cell nucleus; Hydrodynamic filtration; Microfluidics; Subcellular separation

Indexed keywords

BIOCHEMICAL RESEARCH; BIOLOGICAL APPLICATIONS; CARRIER MEDIUM; CELL NUCLEUS; CELL SUSPENSION; CELL TREATMENT; CHEMICAL TREATMENTS; FLOWTHROUGH; HIGH REPRODUCIBILITY; MICRO-FLUIDIC DEVICES; PRECISE CONTROL; RECOVERY RATIO; SELECTIVE DIGESTION; SIDE-CHANNEL; SUB-CELLULAR; SURFACTANT MOLECULES; TREATMENT TIME; TRITON X-100; WASHING BUFFERS; WESTERN BLOTTING;

CELL CULTURE; CELL MEMBRANES; DEBRIS; HYDRODYNAMICS; MICROCHANNELS; MICROFLUIDICS; SURFACE ACTIVE AGENTS;

MOLECULAR BIOLOGY;

BUFFER; DODECYL SULFATE SODIUM; SURFACTANT; TRITON X 100;

ARTICLE; CELL ADHESION; CELL COMPONENT; CELL ISOLATION; CELL METABOLISM; CELL NUCLEUS; CELL NUCLEUS MEMBRANE; CELL SEPARATION; CELL SIZE; CELL STRUCTURE; CELL SUSPENSION; CHEMICAL ANALYSIS; FLOW RATE; HUMAN; HUMAN CELL; HYDRODYNAMIC FILTRATION; HYDRODYNAMICS; LAB ON A CHIP; LABORATORY DEVICE; MEMBRANE CHANNEL; MICROFLUIDIC ANALYSIS; MICROFLUIDIC DEVICE; PRIORITY JOURNAL; REPRODUCIBILITY; TWO STEP CARRIER MEDIUM EXCHANGE; WESTERN BLOTTING;

CELL FRACTIONATION; CELL NUCLEUS; HELA CELLS; HUMANS; MICROFLUIDIC ANALYTICAL TECHNIQUES; OCTOXYNOL; SODIUM DODECYL SULFATE; SURFACE-ACTIVE AGENTS; TIME FACTORS;

EID: 84865436958     PISSN: 13872176     EISSN: 15728781     Source Type: Journal    
DOI: 10.1007/s10544-012-9653-8     Document Type: Article

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