Identification of intrinsically disordered proteins by a special 2D electrophoresis

Methods in Molecular Biology

Volumn 896, Issue , 2012, Pages 215-222

  Tantos, Agnes ^a   Tompa, Peter ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

2D electrophoresis; Diagonal electrophoresis; Heat stable proteins; Intrinsically unstructured protein; Large scale separation; Proteomics

Indexed keywords

PROTEIN;

ARTICLE; CHEMISTRY; ISOLATION AND PURIFICATION; METHODOLOGY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; PROTEINS;

EID: 84865340391     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4614-3704-8_13     Document Type: Article

References (14)

1. Uversky VN (2002) Natively unfolded proteins: a point where biology waits for physics. Protein Sci 11:739-756
2. Wright PE, Dyson HJ (1999) Intrinsically unstructured proteins: re-assessing the protein structure-function paradigm. J Mol Biol 293:321-331
3. Dunker AK, Brown CJ, Lawson JD, Iakoucheva LM, Obradovic Z (2002) Intrinsic disorder and protein function. Biochemistry 41:6573-6582
4. Tompa P (2002) Intrinsically unstructured proteins. Trends Biochem Sci 27:527-533
5. Dunker AK, Obradovic Z, Romero P, Garner EC, Brown CJ (2000) Intrinsic protein disorder in complete genomes. Genome InformSer Workshop Genome Inform 11:161-171
6. Kim TD, Ryu HJ, Cho HI, Yang CH, Kim J (2000) Thermal behavior of proteins: heatresistant proteins and their heat-induced secondary structural changes. Biochemistry 39:14839-14846
7. Tompa P, Csermely P (2004) The role of structural disorder in the function of RNA and protein chaperones. FASEB J 18:1169-1175
8. Hackel M, Konno T, Hinz H (2000) A new alternative method to quantify residual structure in 'unfolded' proteins. Biochim Biophys Acta 1479:155-165
9. Weinreb PH, ZhenW, Poon AW, Conway KA, Lansbury PT Jr (1996) NACP, a protein implicated in Alzheimer's disease and learning, is natively unfolded. Biochemistry 35:13709-13715
10. Belmont LD, Mitchison TJ (1996) Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules. Cell 84:623-631
11. Kriwacki RW, Hengst L, Tennant L, Reed SI, Wright PE (1996) Structural studies of p21Waf1/Cip1/Sdi1 in the free and Cdk2-bound state: conformational disorder mediates binding diversity. Proc Natl Acad Sci USA 93:11504-11509
12. Szollosi E, Bokor M, Bodor A, Perczel A, Klement E, Medzihradszky KF, Tompa K, Tompa P (2008) Intrinsic structural disorder of DF31, a Drosophila protein of chromatin decondensation and remodeling activities. J Proteome Res 7:2291-2299
13. Csizmok V, Szollosi E, Friedrich P, Tompa P (2006) A novel two-dimensional electrophoresis technique for the identification of intrinsically unstructured proteins. Mol Cell Proteomics 5:265-273
14. Uversky VN, Gillespie JR, Fink AL (2000) Why are "natively unfolded" proteins unstructured under physiologic conditions? Proteins 41:415-427