Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells

Experimental Biology and Medicine

Volumn 237, Issue 7, 2012, Pages 845-852

  Li, Hao ^a   Liang, Chengzhen ^a   Tao, Yiqing ^a   Zhou, Xiaopeng ^a   Li, Fangcai ^a   Chen, Gang ^a   Chen, Qi xin ^a  

^a ZHEJIANG UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

Acidity; Adipose derived mesenchymal stem cells; Intervertebral disc

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; AGGRECAN; CASPASE 3; COLLAGEN TYPE 1; COLLAGEN TYPE 2; FLUORESCEIN ISOTHIOCYANATE; GELATINASE A; LIPOCORTIN 5; MESSENGER RNA; PROPIDIUM IODIDE; TISSUE INHIBITOR OF METALLOPROTEINASE 3;

ADIPOSE DERIVED STEM CELL; ADULT; ARTICLE; CELL FUNCTION; CELL PH; CELL PROLIFERATION; CELL SURVIVAL; CELL VIABILITY; CLINICAL ARTICLE; CONTROLLED STUDY; DISEASE SEVERITY; ENVIRONMENT; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERVERTEBRAL DISK DEGENERATION; MALE; MESENCHYMAL STEM CELL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION;

ACIDS; ADIPOSE TISSUE; BASE SEQUENCE; CELL SURVIVAL; DNA PRIMERS; ENZYME-LINKED IMMUNOSORBENT ASSAY; EXTRACELLULAR MATRIX PROTEINS; HUMANS; HYDROGEN-ION CONCENTRATION; INTERVERTEBRAL DISC DEGENERATION; MESENCHYMAL STEM CELLS; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 84865328332     PISSN: 15353702     EISSN: 15353699     Source Type: Journal    
DOI: 10.1258/ebm.2012.012009     Document Type: Article

References (26)

1. Andersson GB. Epidemiological features of chronic low-back pain. Lancet 1999;354:581-5.
2. Martell BA, O'Connor PG, Kerns RD, Becker WC, Morales KH, Kosten TR, Fiellin DA. Systematic review: opioid treatment for chronic back pain: prevalence, efficacy, and association with addiction. Ann Intern Med 2007;146:116-27.
3. Katz JN. Lumbar disc disorders and low-back pain: socioeconomic factors and consequences. J Bone Joint Surg Am 2006;88(Suppl 2):21-4.
4. Serigano K, Sakai D, Hiyama A, Tamura F, Tanaka M, Mochida J. Effect of cell number on mesenchymal stem cell transplantation in a canine disc degeneration model. J Orthop Res 2010;28:1267-75.
5. Ganey T, Hutton WC, Moseley T, Hedrick M, Meisel HJ. Intervertebral disc repair using adipose tissue-derived stem and regenerative cells: experiments in a canine model. Spine (Phila Pa 1976) 2009;34:2297-304.
6. Gruber HE, Hanley EN Jr. Recent advances in disc cell biology. Spine (Phila Pa 1976) 2003;28:186-93.
7. Sakai D. Future perspectives of cell-based therapy for intervertebral disc disease. Eur Spine J 2008;17(Suppl 4):452-8.
8. Wu X, Liu X, Wang S. Implantation of biomaterial as an efficient method to harvest mesenchymal stem cells. Exp Biol Med (Maywood) 2011;236:1477-84.
9. Wei Y, Sun X, Wang W, Hu Y. Adipose-derived stem cells and chondrogenesis. Cytotherapy 2007;9:712-6.
10. Musumeci G, Lo Furno D, Loreto C, Giuffrida R, Caggia S, Leonardi R, Cardile V. Mesenchymal stem cells from adipose tissue which have been differentiated into chondrocytes in three-dimensional culture express lubricin. Exp Biol Med (Maywood) 2011;236:1333-41.
11. Tapp H, Hanley EN Jr., Patt JC, Gruber HE. Adipose-derived stem cells: characterization and current application in orthopaedic tissue repair. Exp Biol Med (Maywood) 2009;234:1-9.
12. Jeong JH, Lee JH, Jin ES, Min JK, Jeon SR, Choi KH. Regeneration of intervertebral discs in a rat disc degeneration model by implanted adipose-tissue-derived stromal cells. Acta Neurochir (Wien) 2010;152:1771-7.
13. Razaq S, Wilkins RJ, Urban JP. The effect of extracellular pH on matrix turnover by cells of the bovine nucleus pulposus. Eur Spine J 2003;12:341-9.
14. Wuertz K, Godburn K, Iatridis JC. MSC response to pH levels found in degenerating intervertebral discs. Biochem Biophys Res Commun 2009;379:824-9.
15. Nachemson A. Intradiscal measurements of pH in patients with lumbar rhizopathies. Acta Orthop Scand 1969;40:23-42.
16. Ichimura K, Tsuji H, Matsui H, Makiyama N. Cell culture of the intervertebral disc of rats: factors influencing culture, proteoglycan, collagen, and deoxyribonucleic acid synthesis. J Spinal Disord 1991;4:428-36.
17. Diamant B, Karlsson J, Nachemson A. Correlation between lactate levels and pH in discs of patients with lumbar rhizopathies. Experientia 1968;24:1195-6.
18. Kitano T, Zerwekh JE, Usui Y, Edwards ML, Flicker PL, Mooney V. Biochemical changes associated with the symptomatic human intervertebral disk. Clin Orthop Relat Res 1993;372-7.
19. Ohshima H, Urban JP. The effect of lactate and pH on proteoglycan and protein synthesis rates in the intervertebral disc. Spine (Phila Pa 1976) 1992;17:1079-82.
20. Smith LJ, Nerurkar NL, Choi KS, Harfe BD, Elliott DM. Degeneration and regeneration of the intervertebral disc: lessons from development. Dis Model Mech 2011;4:31-41.
21. Acosta FL Jr., Metz L, Adkisson HD, Liu J, Carruthers-Liebenberg E, Milliman C, Maloney M, Lotz JC. Porcine intervertebral disc repair using allogeneic juvenile articular chondrocytes or mesenchymal stem cells. Tissue Eng Part A 2011;17:3045-55.
22. Razaq S, Urban JP, Wilkins RJ. Regulation of intracellular pH by bovine intervertebral disc cells. Cell Physiol Biochem 2000;10:109-15.
23. Perez-Sala D, Collado-Escobar D, Mollinedo F. Intracellular alkalinization suppresses lovastatin-induced apoptosis in HL-60 cells through the inactivation of a pH-dependent endonuclease. J Biol Chem 1995;270:6235-42.
24. Lucas CA, Gillies RJ, Olson JE, Giuliano KA, Martinez R, Sneider JM. Intracellular acidification inhibits the proliferative response in BALB/ c-3T3 cells. J Cell Physiol 1988;136:161-7.
25. Nerlich AG, Schleicher ED, Boos N. 1997 Volvo Award winner in basic science studies. Immunohistologic markers for age-related changes of human lumbar intervertebral discs. Spine (Phila Pa 1976) 1997;22:2781-95.
26. Hohaus C, Ganey TM, Minkus Y, Meisel HJ. Cell transplantation in lumbar spine disc degeneration disease. Eur Spine J 2008;17(Suppl 4): 492-503.