A sensitive switch for visualizing natural gene silencing in single cells

ACS Synthetic Biology

Volumn 1, Issue 3, 2012, Pages 99-106

  Haynes, Karmella A ^a   Ceroni, Francesca ^b   Flicker, Daniel ^c   Younger, Andrew ^c   Silver, Pamela A ^c  

^a Arizona State University   (United States)

^b UNIVERSITY OF BOLOGNA   (Italy)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Genetic switch; MiR 34; RNA interference; Synthetic repressor

Indexed keywords

MICRORNA;

ARTICLE; DYNAMICS; ENZYME ACTIVITY; GENE SILENCING; MAMMAL CELL; NONHUMAN; OSTEOSARCOMA CELL; PRIORITY JOURNAL; RNA INTERFERENCE; SINGLE CELL ANALYSIS;

CELL LINE; GENE SILENCING; GENES, REPORTER; GENETIC TECHNIQUES; HUMANS; LUMINESCENT PROTEINS; MICRORNAS; MODELS, GENETIC; RECOMBINANT FUSION PROTEINS; RNA INTERFERENCE; SYNTHETIC BIOLOGY;

MAMMALIA;

EID: 84865304769     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb3000035     Document Type: Article

References (30)

1. Greber, D., and Fussenegger, M. (2007) Mammalian synthetic biology: engineering of sophisticated gene networks. J. Biotechnol. 130, 329-345. (Pubitemid 47021243)
2. Haynes, K. A., and Silver, P. A. (2009) Eukaryotic systems broaden the scope of synthetic biology. J. Cell Biol. 187, 589-596.
3. Ye, H., Daoud-El Baba, M., Peng, R.-W., and Fussenegger, M. A Synthetic Optogenetic Transcription Device Enhances Bloodglucose Homeostasis In Mice. Science 332 1565-1568.
4. Xie, Z., Wroblewska, L., Prochazka, L., Weiss, R., and Benenson, Y. (2011) Multi-Input RNAi-Based Logic Circuit for Identification of Specific Cancer Cells. Science 333, 1307-1311.
5. Boyerinas, B., Park, S.-M., Hau, A., Murmann, A. E., and Peter, M. E. (2010) The role of let-7 in cell differentiation and cancer. Endocr. Relat. Cancer 17, F19-36.
6. Lin, S.-Y., Johnson, S. M., Abraham, M., Vella, M. C., Pasquinelli, A., Gamberi, C., Gottlieb, E., and Slack, F. J. (2003) The C elegans hunchback homolog, hbl-1, controls temporal patterning and is a probable microRNA target. Develop. Cell 4, 639-650. (Pubitemid 36564895)
7. Nimmo, R. A., and Slack, F. J. (2009) An elegant miRror: microRNAs in stem cells, developmental timing and cancer. Chromosoma 118, 405-418.
8. Merkerova, M., Belickova, M., and Bruchova, H. (2008) Differential expression of microRNAs in hematopoietic cell lineages. Eur. J. Haematol. 81, 304-310.
9. Sun, F., Fu, H., Liu, Q., Tie, Y., Zhu, J., Xing, R., Sun, Z., and Zheng, X. (2008) Downregulation of CCND1 and CDK6 by miR-34a induces cell cycle arrest. FEBS Lett. 582, 1564-1568.
10. Lynam-Lennon, N., Maher, S. G., and Reynolds, J. V. (2009) The roles of microRNA in cancer and apoptosis. Biol. Rev. 84, 55-71.
11. Cullen, B. (2004) Transcription and Processing of Human microRNA Precursors. Mol. Cell 16, 861-865. (Pubitemid 40018397)
12. Koutsoulidou, A., Mastroyiannopoulos, N. P., Furling, D., Uney, J. B., and Phylactou, L. A. (2011) Expression of miR-1, miR-133a, miR-133b and miR-206 increases during development of human skeletal muscle. BMC Dev. Biol. 11, 34.
13. Gokey, N. G., Srinivasan, R., Lopez-Anido, C., Krueger, C., and Svaren, J. (2011) Developmental Regulation of MicroRNA Expression in Schwann Cells. Mol. Cell Biol. 32, 558-568.
14. Yang, M., Lee, J.-E., Padgett, R. W., and Edery, I. (2008) Circadian regulation of a limited set of conserved microRNAs in Drosophila. BMC Genomics 9, 83.
15. Cheng, H.-Y. M., and Obrietan, K. (2007) Revealing a role of microRNAs in the regulation of the biological clock. Cell Cycle 6, 3034-3035.
16. De Pietri Tonelli, D., Calegari, F., Fei, J.-F., Nomura, T., Osumi, N., Heisenberg, C.-P., and Huttner, W. B. (2006) Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid. Biotech 41, 727-732. (Pubitemid 46534858)
17. Zhang, X., Zabinsky, R., Teng, Y., Cui, M., and Han, M. (2011) microRNAs play critical roles in the survival and recovery of Caenorhabditis elegans from starvation-induced L1 diapause. Proc. Natl. Acad. Sci. U.S.A. 108, 17997-18002.
18. Varghese, J., Lim, S. F., and Cohen, S. M. (2010) Drosophila miR-14 regulates insulin production and metabolism through its target, sugarbabe. Genes Dev. 24, 2748-2753.
19. Milo, R., Jorgensen, P., Moran, U., Weber, G., and Springer, M. (2010) BioNumbers-the database of key numbers in molecular and cell biology. Nucleic Acids Res. 38, D750-3.
20. Rinaudo, K., Bleris, L., Maddamsetti, R., Subramanian, S., Weiss, R., and Benenson, Y. (2007) A universal RNAi-based logic evaluator that operates in mammalian cells. Nat. Biotechnol. 25, 795-801. (Pubitemid 47052185)
21. Kramer, B. P., Viretta, A. U., Baba, M. D.-E., Aubel, D., Weber, W., and Fussenegger, M. (2004) An engineered epigenetic transgene switch in mammalian cells. Nat. Biotechnol. 22, 867-870. (Pubitemid 38886606)
22. Ryan, R. F., Schultz, D. C., Ayyanathan, K., Singh, P. B., Friedman, J. R., Fredericks, W. J., and Rauscher, F. J. (1999) KAP-1 corepressor protein interacts and colocalizes with heterochromatic and euchromatic HP1 proteins: a potential role for Kruppel-associated boxzinc finger proteins in heterochromatin-mediated gene silencing. Mol. Cell. Biol. 19, 4366-4378. (Pubitemid 29242011)
23. Ramos, J. L., Martínez-Bueno, M., Molina-Henares, A. J., Teran, W., Watanabe, K., Zhang, X., Gallegos, M. T., Brennan, R., and Tobes, R. (2005) The TetR family of transcriptional repressors. Microbiol. Mol. Biol. Rev. 69, 326-356. (Pubitemid 40847943)
24. Lewis, M. (2005) The lac repressor. C. R. Biol. 328, 521-548. (Pubitemid 40796583)
25. Shih, J. D., Waks, Z., Kedersha, N., and Silver, P. A. (2011) Visualization of single mRNAs reveals temporal association of proteins with microRNA-regulated mRNA. Nucleic Acids Res. 39, 7740-7749.
26. Chung, K. H. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. Nucleic Acids Res. 34, e53-e53.
27. He, C., Xiong, J., Xu, X., Lu, W., Liu, L., Xiao, D., and Wang, D. (2009) Functional elucidation of MiR-34 in osteosarcoma cells and primary tumor samples. Biochem. Biophys. Res. Commun. 388, 35-40.
28. Phillips, I., and Silver, P. (2006) DSpace@MIT: A New Biobrick Assembly Strategy Designed for Facile Protein Engineering, http://hdl.handle.net/1721.1/ 32535.
29. Endy, D. (2005) Foundations for engineering biology. Nat. Cell Biol. 438, 449-453. (Pubitemid 41742010)
30. Raymond, C. K., Roberts, B. S., Garrett-Engele, P., Lim, L. P., and Johnson, J. M. (2005) Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs. RNA 11, 1737-1744. (Pubitemid 41505548)