Performance of the Sysmex UF1000i system in screening for significant bacteriuria before quantitative culture of aerobic/facultative fast-growth bacteria in a reference hospital

Journal of Applied Microbiology

Volumn 113, Issue 3, 2012, Pages 609-614

  Gutierrez Fernandez J ^a,b   Lara, A ^a   Bautista, M F ^a   de Dios Luna, J ^b   Polo, P ^a   Miranda, C ^a   Navarro, J M ^a  

^a HOSPITAL UNIVERSITARIO VIRGEN DE LAS NIEVES   (Spain)

^b UNIVERSITY OF GRANADA   (Spain)

Author keywords

Bacteriuria; Cytometry; Sysmex; Urinary tract infection; Urine

Indexed keywords

BODY FLUIDS; SCREENING;

BACTERIURIA; COLONY FORMING UNITS; CYTOMETRY; FAST GROWTHS; PERFORMANCE; POSITIVE PREDICTIVE VALUES; SCREENING SYSTEM; SYSMEX; URINARY TRACT INFECTIONS; URINE;

AEROBIC BACTERIA;

BACTERIAL DISEASE; BACTERIOLOGY; BACTERIUM; COLONY; FLOW CYTOMETRY; GROWTH RATE; HOSPITAL SECTOR; PERFORMANCE ASSESSMENT; URINE;

ANALYZER; ARTICLE; BACTERIAL COUNT; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM EXAMINATION; BACTERIURIA; COLONY FORMING UNIT; CONTROLLED STUDY; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ESCHERICHIA COLI; FALSE NEGATIVE RESULT; FEMALE; FLOW CYTOMETRY; HOSPITAL; HUMAN; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; LEUKOCYTE COUNT; LEUKOCYTURIA; MAJOR CLINICAL STUDY; MORGANELLA MORGANII; PROTEUS MIRABILIS; PROVIDENCIA STUARTII; PSEUDOMONAS AERUGINOSA; SCREENING TEST; STAPHYLOCOCCUS SAPROPHYTICUS; STREPTOCOCCUS AGALACTIAE;

NUCLEOPOLYHEDROVIRUS;

EID: 84865304108     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2012.05369.x     Document Type: Article

References (20)

1. Andreu, A., Cacho, J., Coira, A. and Lepe, J.A. (2011). Microbiological diagnosis of urinary tract infections. Enferm Infecc Microbiol Clin 29, 52-57.
2. Baron, E.J., Thomson, R.B. (2011) Specimen collection, transport, and processing: bacteriology. Volume 1, Chapter 16. In Manual of Clinical Microbiology, 10th edn ed. Versalovic, J., Carroll, K.C., Funke, G., Jorgensen, J.H., Landry, M.L. and Warnock, D.W., pp. 228-271. Washington, DC: ASM Press.
3. Broeren, M.C., Bahçeci, S., Vader, H.L. and Arents, N.L. (2011). Screening for urinary tract infection with the Sysmex UF-1000i urine flow cytometer. J Clin Microbiol 49, 1025-1029.
4. Falbo, R., Sala, M.R., Signorelli, S., Venturi, N., Signorini, S. and Brambilla, P. (2012) Bacteriuria screening by automated whole-field-image-based microscopy reduces the number of necessary urine cultures. J Clin Microbiol 50, 1427-1429.
5. Farrar, J.T., Young, J.P., La Moreaux, L., Werth, J.L. and Poole, R.M. (2001). Clinical importance of changes in chronic pain intensity measured on an 11-point numerical pain rating scale. Pain 94, 149-158.
6. Gutiérrez, J., Rodríguez, M., Morales, P. and Piédrola, G. (2000). Importance of risk of contamination from urine samples for diagnosis of urinary tract infection in a third level hospital. Rev Invest Clin 52, 581-583.
7. Gutiérrez, J., Sorlózano, A., Soto, M.J. and Piédrola, G. (2006). Evaluation of two automated systems for detection of bacteriuria. J Clin Lab Anal 20, 118-120.
8. Hanley, J.A. and McNeil, B.J. (1982). The meaning and use of the area under a receiver operating characteristic (ROC) curve. Radiology 143, 29-36.
9. Jiang, T., Chen, P., Ouyang, J., Zhang, S. and Cai, D. (2011). Urine particles analysis: performance evaluation of Sysmex UF-1000i and comparison among urine flow cytometer, dipstick, and visual microscopic examination. Scand J Clin Lab Invest 71, 30-37.
10. Jolkkonen, S., Paattiniemi, E.L., Kärpänoja, P. and Sarkkinen, H. (2010). Screening of urine samples by flow cytometry reduces the need for culture. J Clin Microbiol 48, 3117-3121.
11. De Long, E.R., De Long, D.M. and Clarke-Pearson, D.L. (1988). Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach. Biometrics 44, 837-845.
12. Manoni, F., Fornasiero, L., Ercolin, M., Tinello, A., Ferrian, M., Hoffer, P., Valverde, S. and Gessoni, G. (2009). Cutoff values for bacteria and leukocytes for urine flow cytometer Sysmex UF-1000i in urinary tract infections. Diagn Microbiol Infect Dis 65, 103-107.
13. Marschal, M., Wienke, M., Hoering, S., Autenrieth, I.B. and Frick, J.S. (2012). Evaluation of 3 different rapid automated systems for diagnosis of urinary tract infections. Diagn Microbiol Infect Dis 72, 125-130.
14. Palacios, E., Rodríguez-Granjer, J., Sampedro, A., Martínez-Brocal, A. and De La Rosa, M. (2002). Use of MPO chromogenic culture medium for routine processing of urine cultures. Enferm Infecc Microbiol Clin 20, 388-390.
15. Pezzlo, M., Mary, K.C. and Deirdre, L.C. (2010) Urine cultures. Volume 1, Chapter 3.12. In Clinical Microbiology Procedures Handbook, 3th edn Ed. Garcia, L.S. Washington, DC: ASM Press.
16. Pieretti, B., Brunati, P., Pini, B., Colzani, C., Congedo, P., Rocchi, M. and Terramocci, R. (2010). Diagnosis of bacteriuria and leukocyturia by automated flow cytometry compared with urine culture. J Clin Microbiol 48, 3990-3996.
17. De Rosa, R., Grosso, S., Bruschetta, G., Avolio, M., Stano, P., Modolo, M.L. and Camporese, A. (2010). Evaluation of the Sysmex UF1000i flow cytometer for ruling out bacterial urinary tract infection. Clin Chim Acta 411, 1137-1142.
18. Sobel, J.D. and Kaye, D. (2010) Urinary tract infections. Volume I, Part II, Chapter 69. In Mandell, Douglas and Bennett's Principles and Practice of Infectious Diseases, 7th edn ed. Mandell, G.L., Bennett, J.E. and Dolin, R. pp. 957-985. Philadelphia, PA: Churchill Livingstone Elsevier.
19. Wang, J., Zhang, Y., Xu, D., Shao, W. and Lu, Y. (2010). Evaluation of the Sysmex UF-1000i for the diagnosis of urinary tract infection. Am J Clin Pathol 133, 577-582.
20. ® urine flow cytometer in the diagnostic work-up of suspected urinary tract infection in a Dutch general hospital. Clin Chem Lab Med 48, 1765-1771.