Programmable illumination and high-speed, multi-wavelength, confocal microscopy using a digital micromirror

PLoS ONE

Volumn 7, Issue 8, 2012, Pages

  Martial, Franck P ^a   Hartell, Nicholas A ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; RED FLUORESCENT PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIOMEDICAL TECHNOLOGY ASSESSMENT; CONFOCAL MICROSCOPY; DIGITAL IMAGING; DIGITAL MICROMIRROR DEVICE; FLUORESCENCE MICROSCOPY; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; ILLUMINATION; IMAGE ANALYSIS; IMAGE DISPLAY; INTERMETHOD COMPARISON; LIGHT EMITTING DIODE; LIGHT INTENSITY; MALE; MOLECULAR IMAGING; NONHUMAN; OPTICAL INSTRUMENTATION; OPTICAL RESOLUTION; PHOTOACTIVATION; RAT;

CALCIUM; IMAGE PROCESSING, COMPUTER-ASSISTED; LIGHTING; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE;

EID: 84865298177     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0043942     Document Type: Article

References (16)

1. Conchello JA, Lichtman JW, (2005) Optical sectioning microscopy. NatMethods 2: 920-931.
2. Callamaras N, Parker I, (1999) Construction of line-scan confocal microscope for physiological recording. Methods Enzymol 307: 152-169.
3. Nakano A, (2002) Spinning-disk confocal microscopy - a cutting-edge tool for imaging of membrane traffic. Cell StructFunct 27: 349-355.
4. Liang M, Stehr R, Krause A, (1997) Confocal pattern period in multiple-aperture confocal imaging systems with coherent illumination. OptLett 22: 751-753.
5. Hanley QS, Verveer PJ, Gemkow MJ, rndt-Jovin D, Jovin TM, (1999) An optical sectioning programmable array microscope implemented with a digital micromirror device. J Microsc 196 (Pt 3): 317-331.
6. Heintzmann R, Hanley QS, rndt-Jovin D, Jovin TM, (2001) A dual path programmable array microscope (PAM): simultaneous acquisition of conjugate and non-conjugate images. J Microsc 204: 119-135.
7. Fukano T, Miyawaki A, (2003) Whole-field fluorescence microscope with digital micromirror device: imaging of biological samples. ApplOpt 42: 4119-4124.
8. Bansal V, Patel S, Saggau P, (2004) High-speed confocal laser scanning microscopy using acousto-optic deflectors and a digital micromirror device. Procedings- SPIE The International Society for Optical Engineering 5324: 47-54.
9. Bansal V, Patel S, Saggau P, (2006) High-speed addressable confocal microscopy for functional imaging of cellular activity. JBiomedOpt 11: 34003.
10. Jiang S, Walker J, (2010) Differential high-speed digital micromirror device based fluorescence speckle confocal microscopy. ApplOpt 49: 497-504.
11. Jiang Sh, Walker JG, (2005) Non-scanning fluorescence confocal microscopy using speckle illumination and optical data processing. Optics Communications 256: 35-45.
12. Hagen GM, Caarls W, Thomas M, Hill A, Lidke KA, et al. (2007) Biological applications of an LCoS-based programmable array microscope (PAM). Proc SPIE 6441.
13. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, et al. (2004) Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. NatBiotechnol 22: 1567-1572.
14. Tallini YN, Ohkura M, Choi BR, Ji G, Imoto K, et al. (2006) Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2. ProcNatlAcadSciUSA 103: 4753-4758.
15. McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL, (2009) A bright and photostable photoconvertible fluorescent protein. Nat Meth 6: 131-133.
16. Eilers J, Callewaert G, Armstrong C, Konnerth A, (1995) Calcium signaling in a narrow somatic submembrane shell during synaptic activity in cerebellar Purkinje neurons. ProcNatlAcadSciUSA 92: 10272-10276.