Structure-guided mutagenesis for the improvement of substrate specificity of Bacillus megaterium glucose 1-dehydrogenase IV

FEBS Journal

Volumn 279, Issue 17, 2012, Pages 3264-3275

  Nishioka, Taiki ^a   Yasutake, Yoshiaki ^b   Nishiya, Yoshiaki ^c   Tamura, Tomohiro ^a,b  

^a HOKKAIDO UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c TOYOBO CO LTD   (Japan)

Author keywords

Bacillus megaterium; crystal structure; glucose 1 dehydrogenase; short chain dehydrogenase reductase; site directed mutagenesis; substrate specificity

Indexed keywords

AMINO ACID; GLUCOSE; GLYCOSE 1 DEHYDROGENASE IV; OXIDOREDUCTASE; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS MEGATERIUM; BACTERIUM MUTANT; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME CONFORMATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; HYDROPHOBICITY; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; X RAY DIFFRACTION;

BACILLUS MEGATERIUM; GLUCOSE; GLUCOSE 1-DEHYDROGENASE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; SUBSTRATE SPECIFICITY;

BACILLUS MEGATERIUM; BACILLUS SP.;

EID: 84865268524     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2012.08713.x     Document Type: Article

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