Site directed mutagenesis studies of FAD-dependent glucose dehydrogenase catalytic subunit of Burkholderia cepacia

Biotechnology Letters

Volumn 30, Issue 11, 2008, Pages 1967-1972

  Yamaoka, Hideaki ^a   Yamashita, Yuki ^a   Ferri, Stefano ^a   Sode, Koji ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

Diabetes; Glucose dehydrogenase; Glucose sensor; Substrate specificity

Indexed keywords

ENZYME ACTIVITY; ENZYME SENSORS; ENZYMES; FOOD ADDITIVES; GLUCOSE; GLUCOSE OXIDASE; MUTAGENESIS; SUBSTRATES;

ACTIVE SITES; BURKHOLDERIA CEPACIA; CATALYTIC ACTIVITIES; CATALYTIC SUBUNITS; DIABETES; ELECTRON TRANSFER ABILITY; GLUCOSE DEHYDROGENASE; GLUCOSE OXIDASES; GLUCOSE SENSOR; SITE-DIRECTED MUTAGENESIS; SUBSTRATE SPECIFICITY; WILD-TYPE ENZYME;

GLUCOSE SENSORS;

ALANINE; ASPARAGINE; FLAVINE ADENINE NUCLEOTIDE; GLUCOSE; GLUCOSE DEHYDROGENASE;

AMINO ACID SEQUENCE; ARTICLE; BURKHOLDERIA CEPACIA; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

ALANINE; AMINO ACID SEQUENCE; ASPARAGINE; BASE SEQUENCE; BURKHOLDERIA CEPACIA; CATALYTIC DOMAIN; FLAVIN-ADENINE DINUCLEOTIDE; GLUCOSE; GLUCOSE 1-DEHYDROGENASE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

BURKHOLDERIA CEPACIA;

EID: 52449112953     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9777-3     Document Type: Article

References (7)

1. K Inose M Fujikawa T Yaniazaki K Kojima K Sode 2003 Cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from Burkholderia Cepacia in Escherichia coli Biochim Biophys Acta (Proteins and Proteomics) 1645 133 138
2. W Janssen G Harff M Caers A Schellekens 1998 Positive interference of icodextrin metabolites in some enzymatic glucose methods Clin Chem 44 2379 2380
3. K Sode W Tsugawa T Yamazaki M Watanabe N Ogasawara M Tanaka 1996 A novel thermostable glucose dehydrogenase varying temperature properties by altering its quaternary structures Enzyme Microbial Technol 19 82 85
4. T Tsuya S Ferri M Fujikawa H Yamaoka K Sode 2006 Cloning and functional expression of glucose dehydrogenase complex of Burkholderia Cepacia in Escherichia coli J Biotechnol 123 127 136
5. G Wohlfahrt S Witt J Hendle D Schomburg HM Kalisz HJ Hecht 1999 1.8 and 1.9 a resolution structures of the Penicillium amagasakiense and Aspergillus niger glucose oxidases as a basis for modelling substrate complexes Acta Cryst D55 969 977
6. H Yamaoka K Sode 2007 SPCE based glucose sensor employing novel thermostable glucose dehydrogenase, FADGDH: blood glucose measurement with 150 nL sample in one-second J Diabetes Sci Technol 1 28 35
7. H Yamaoka K Sode 2007 A disposable electrochemical glucose sensor using catalytic subunit of novel thermostable glucose dehydrogenase Open Biotechnol J 1 26 30