Demonstration of cell membrane permeabilization to medium-sized molecules caused by a single 10ns electric pulse

Bioelectrochemistry

Volumn 87, Issue , 2012, Pages 260-264

  Silve, Aude ^a   Leray, Isabelle ^b   Mir, Lluis M ^c  

^a UNIVERSITÉ PARIS SACLAY   (France)

^b CNRS   (France)

^c INSTITUT GUSTAVE ROUSSY   (France)

Author keywords

Bleomycin; Electropermeabilization; Electroporation; Nanopulse; NsPEF

Indexed keywords

BLEOMYCINS; ELECTROPERMEABILIZATION; ELECTROPORATION; NANOPULSE; NSPEF;

CELL DEATH; CELL MEMBRANES; DEFECTS; MOLECULES;

PEPTIDES;

BLEOMYCIN;

ANIMAL CELL; ARTICLE; CELL DEATH; CELL MEMBRANE; CELL MEMBRANE PERMEABILIZATION; CELL PERMEABILIZATION; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; ELECTRIC FIELD; ELECTROPORATION; NANOPORE; NONHUMAN;

ANIMALS; BLEOMYCIN; CELL LINE; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CELL NUCLEUS; CELL SURVIVAL; CRICETINAE; CRICETULUS; CYTOPLASM; ELECTRICITY; ELECTROPORATION; LUNG; MICROSCOPY;

EID: 84865196842     PISSN: 15675394     EISSN: 1878562X     Source Type: Journal    
DOI: 10.1016/j.bioelechem.2011.10.002     Document Type: Article

References (20)

1. Deng J., Schoenbach K.H., Buescher E.S., Hair P.S., Fox P.M., Beebe S.J. The effects of intense submicrosecond electrical pulses on cells. Biophys. J. 2003, 84:2709-2714.
2. Schoenbach K.H., Beebe S.J., Buescher E.S. Intracellular effect of ultrashort electrical pulses. Bioelectromagnetics 2001, 22:440-448.
3. Vernier P.T., Sun Y., Marcu L., Salemi S., Craft C.M., Gundersen M.A. Calcium bursts induced by nanosecond electric pulses. Biochem. Biophys. Res. Commun. 2003, 310:286-295.
4. Bowman A.M., Nesin O.M., Pakhomova O.N., Pakhomov A.G. Analysis of plasma membrane integrity by fluorescent detection of Tl(+) uptake. J. Membr. Biol. 2010, 236:15-26.
5. Vernier P.T., Sun Y., Chen M.-T., Gundersen M.A., Craviso G.L. Nanosecond electric pulse-induced calcium entry into chromaffin cells. Bioelectrochemistry 2008, 73:1-4.
6. Poddevin B., Orlowski S., Belehradek J., Mir L.M. Very high cytotoxicity of bleomycin introduced into the cytosol of cells in culture. Biochem. Pharmacol. 1991, 42:S67-S75. Suppl.
7. Tounekti O., Pron G., Belehradek J., Mir L.M. Bleomycin, an apoptosis-mimetic drug that induces two types of cell death depending on the number of molecules internalized. Cancer Res. 1993, 53:5462-5469.
8. Silve A., Mir L.M. Cell electropermeabilization and cellular uptake of small molecules: the electrochemotherapy concept. Clinical Aspects of Electroporation 2011, 69-82. Springer New York, New York, NY. S.T. Kee, J. Gehl, E.W. Lee (Eds.).
9. Belehradek J., Orlowski S., Ramirez L.H., Pron G., Poddevin B., Mir L.M. Electropermeabilization of cells in tissues assessed by the qualitative and quantitative electroloading of bleomycin. Biochim. Biophys. Acta (BBA) - Biomembranes 1994, 1190:155-163.
10. Pakhomov A.G., Bowman A.M., Ibey B.L., Andre F.M., Pakhomova O.N., Schoenbach K.H. Lipid nanopores can form a stable, ion channel-like conduction pathway in cell membrane. Biochem. Biophys. Res. Commun. 2009, 385:181-186.
11. Nesin O.M., Pakhomova O.N., Xiao S., Pakhomov A.G. Manipulation of cell volume and membrane pore comparison following single cell permeabilization with 60- and 600-ns electric pulses. Biochim. Biophys. Acta 2011, 1808:792-801.
12. Pron G., Mahrour N., Orlowski S., Tounekti O., Poddevin B., Belehradek J., et al. Internalisation of the bleomycin molecules responsible for bleomycin toxicity: a receptor-mediated endocytosis mechanism. Biochem. Pharmacol. 1999, 57:45-56.
13. Silve A., Mir L.M. Exposition of cells in suspension using nanosecond duration electric pulses - detection of permeabilisation by cloning efficiency tests: results and artifacts. Proceedings of XXXth URSI General Assembly and Scientific Symposium, Istanbul, Turkey 2011, communication K.01.05.
14. Silve A., Vezinet R., Mir L.M. Implementation of a broad band, high level electric field sensor in biological exposure device. Proceedings of IEEE International Power modulator high voltage conference, Atlanta, GA 2010, 711-714.
15. Pron G., Belehradek J., Mir L.M. Identification of a plasma membrane protein that specifically binds bleomycin. Biochem. Biophys. Res. Commun. 1993, 194:333-337.
16. Pron G., Belehradek J., Orlowski S., Mir L.M. Involvement of membrane bleomycin-binding sites in bleomycin cytotoxicity. Biochem. Pharmacol. 1994, 48:301-310.
17. Mahrour N., Pologea-Moraru R., Moisescu M.G., Orlowski S., Levêque P., Mir L.M. In vitro increase of the fluid-phase endocytosis induced by pulsed radiofrequency electromagnetic fields: importance of the electric field component. Biochim. Biophys. Acta 2005, 1668:126-137.
18. Moisescu M.G., Leveque P., Verjus M.-A., Kovacs E., Mir L.M. 900 MHz modulated electromagnetic fields accelerate the clathrin-mediated endocytosis pathway. Bioelectromagnetics 2009, 30:222-230.
19. Pakhomov A.G., Bowman A.M., Ibey B.L., Andre F.M., Pakhomova O.N., Schoenbach K.H. Lipid nanopores can form a stable, ion channel-like conduction pathway in cell membrane. Biochem. Biophys. Res. Commun. 2009, 385:181-186.
20. Levine Z.A., Vernier P.T. Life cycle of an electropore: field-dependent and field-independent steps in pore creation and annihilation. J. Membr. Biol. 2010, 236:27-36.