Sensitive and robust detection of citrus greening (huanglongbing) bacterium " Candidatus Liberibacter asiaticus" by DNA amplification with new 16S rDNA-specific primers

Molecular and Cellular Probes

Volumn 26, Issue 5, 2012, Pages 194-197

  Fujikawa, Takashi ^a   Iwanami, Toru ^a  

^a NATIONAL INSTITUTE OF FRUIT TREE SCIENCE   (Japan)

Author keywords

16S rDNA; Citrus greening; Diagnosis; DNA amplification; Huanglongbing; Liberibacter asiaticus

Indexed keywords

DNA 16S; PRIMER DNA;

ARTICLE; BACTERIUM DETECTION; CANDIDATUS LIBERIBACTER SUBSPECIES ASIATICUS; CITRUS; DNA SEQUENCE; DNA TEMPLATE; FALSE NEGATIVE RESULT; GENE AMPLIFICATION; GRAM NEGATIVE BACTERIUM; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY; SENSITIVITY ANALYSIS;

CITRUS; DNA PRIMERS; DNA, RIBOSOMAL; JAPAN; PLANT DISEASES; PLANT LEAVES; POLYMERASE CHAIN REACTION; RHIZOBIACEAE; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS); CANDIDATUS LIBERIBACTER; CANDIDATUS LIBERIBACTER ASIATICUS; CITRUS;

EID: 84865068426     PISSN: 08908508     EISSN: 10961194     Source Type: Journal    
DOI: 10.1016/j.mcp.2012.06.001     Document Type: Article

References (22)

1. Bové J.M. Huanglongbing: a destructive, newly-emerging, century-old disease of citrus. J Plant Pathol 2006, 88:7-37.
2. Jagoueix S., Bové J.M., Garnier M. The phloem-limited bacterium of greening disease of citrus is a member of the α subdivision of the Proteobacteria. Int J Sys Bacteriol 1994, 44:379-386.
3. Garnier M., Bové J.M. Distribution of the huanglongbing (greening) Liberobacter species in fifteen Africans and Asian countries. Proc 13th conf IOCV 1996, 388-391. IOCV, Riverside.
4. Garnier M., Jagoueix-Eveillard S., Cronje P.R., Le Roux H.F., Bové J.M. Genomic characterization of a liberibacter present in an ornamental rutaceous tree, Calodendrum capense, in the Western Cape province of South Africa. Proposal of 'Candidatus Liberibacter africanus subsp. capensis'. Int J Sys Evol Microbiol 2000, 50:2119-2125.
5. Teixeira D.C., Saillard C., Eveillard S., Danet J.L., de Costa P.I., Ayres A.J., et al. 'Candidatus Liberibacter americanus', associated with citrus huanglongbing (greening disease) in São Paulo state. Brazil Int J Syst Evol Microbiol 2005, 55:1857-1862.
6. Halbert SE. The discovery of huanglongbing in Florida. In. Proceedings of 2nd international citrus canker and huanglongbing research workshop, Florida citrus mutual, Orlando; 2005, H-3.
7. Teixeira D.C., Danet J.L., Eveillard S., Martins E.C., De Jesus W.C., Yamamoto P.T., et al. Citrus huanglongbing in São Paulo state, Brazil: PCR detection of the 'Candidatus' Liberibacter species associated with the disease. Mol Cell Probes 2005, 19:173-179.
8. Sechler A., Schuenzel E.L., Cooke P., Donnua S., Thaveechai N., Postnikova E., et al. Cultivation of 'Candidatus Liberibacter asiaticus', 'Ca. L. africanus', and 'Ca L. americanus' associated with huanglongbing. Phytopathology 2009, 99:480-486.
9. Committee on the Strategic Planning for the Florida Citrus Industry: addressing citrus greening disease (huanglongbing) National research council. Strategic planning for the Florida citrus industry: addressing citrus greening 2010, The National Academies Press, Washington, DC. http://www.nap.edu/catalog/12880.html.
10. Jagoueix S., Bové J.M., Garnier M. PCR detection of the two 'Candidatus' Liberobacter species associated with greening disease of citrus. Mol Cell Probes 1996, 10:43-50.
11. Hocquellet A., Toorawa P., Bové J.M., Garnier M. Detection and identification of the two Candidatus Liberibacter species associated with citrus huanglongbing by PCR amplification of ribosomal protein genes of the β-operon. Mol Cell Probes 1999, 13:373-379.
12. Hoy M.A., Jeyaprakash A., Nguyen R. Long PCR is a sensitive method for detecting Liberobacter asiaticum in parasitoids undergoing risk assessment in quarantine. Biol Control 2001, 22:278-287.
13. Iwanami T., Uechi N., Kawano S. Temporal change in the distribution of PCR-positive tissue within the tree canopy of Citrus depressa affected by greening. Kyushu Pl Prot Res 2009, 55:68-75. [in Japanese].
14. Duan Y., Zhou L., Hall D.G., Li W., Doddapaneni H., Lin H., et al. Complete genome sequence of citrus huanglongbing bacterium, 'Candidatus Liberibacter asiaticus' obtained through metagenomics. Mol Plant Microbe Interact 2009, 22:1011-1020.
15. Thompson J.D., Higgins D.G., Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994, 22:4673-4680.
16. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997, 25:4876-4882.
17. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011, 28:2731-2739.
18. Furuya N., Matsukura K., Tomimura K., Okuda M., Miyata S.I., Iwanami T. Sequence homogenicity of the ψserA-trmU-tufB-secE-nusG-rplKAJL-rpoB gene cluster and the flanking regions of 'Candidatus Liberibacter asiaticus' isolates around Okinawa main island in Japan. J Gen Plant Pathol 2010, 76:122-131.
19. Tomimura K., Furuya N., Miyata S.I., Hamashima A., Torigoe H., Murayama Y., et al. Distribution of two distinct genotypes of citrus greening organism in the Ryukyu islands of Japan. Jpn Agr Res Q 2010, 44:151-158.
20. Demeke T., Jenkins G.R. Influence of DNA extraction methods, PCR inhibitors and quantification methods on real-time PCR assay of biotechnology-derived traits. Anal Bioanal Chem 2010, 396:1977-1990.
21. Peist R., Honsel D., Twieling G., Löffert D. PCR inhibitors in plant DNA preparations. QIAGEN News 2001, 3:7-9.
22. Teixeira D.C., Saillard C., Couture C., Martins E.C., Wulff N.A., Eveillard-Jagoueix S., et al. Distribution and quantification of Candidatus Liberibacter americanus, agent of huanglongbing disease of citrus in São Paulo state, Brazil, in leaves of an affected sweet orange tree as determined by PCR. Mol Cell Probes 2008, 22:139-150.