Tissue-specific response of metallothionein and superoxide dismutase in the clam Mactra veneriformis under sublethal mercury exposure

Ecotoxicology

Volumn 21, Issue 6, 2012, Pages 1593-1602

  Fang, Yan ^a,b   Yang, Hongsheng ^a   Liu, Baozhong ^a  

^a INSTITUTE OF OCEANOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Mactra veneriformis; Mercury; Metallothionein; mRNA expression; Superoxide dismutase

Indexed keywords

MERCURY; MESSENGER RNA; METALLOTHIONEIN; SUPEROXIDE DISMUTASE;

BIOMARKER; BIVALVE; GENE EXPRESSION; HOMEOSTASIS; MERCURY (ELEMENT); METALLOTHIONEIN; RNA; TOXICITY;

ARTICLE; CLAM; CONTROLLED STUDY; ENVIRONMENTAL EXPOSURE; ENVIRONMENTAL MONITORING; ENVIRONMENTAL STRESS; ENZYME ACTIVITY; GENE EXPRESSION PROFILING; LETHAL DOSE; MACTRA VENERIFORMIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN HOMEOSTASIS; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; SEQUENCE ANALYSIS; TISSUE SPECIFICITY; TRANSCRIPTION REGULATION;

ANIMALS; BIVALVIA; ENVIRONMENTAL EXPOSURE; MERCURY; METALLOTHIONEIN; RNA, MESSENGER; SUPEROXIDE DISMUTASE; TOXICITY TESTS, SUBACUTE;

BIVALVIA; MACTRA VENERIFORMIS;

EID: 84864882326     PISSN: 09639292     EISSN: 15733017     Source Type: Journal    
DOI: 10.1007/s10646-012-0938-8     Document Type: Article

References (56)

1. Baudrimont M, Métivaud J, Maury-Brachet R, Ribeyre F, Boudou A (1997) Bioaccumulation and metallothionein response in the Asiatic clam (Corbicula fluminea) after experimental exposure to cadmium and inorganic mercury. Environ Toxicol Chem 16: 2096-2105
2. Beck F, Neuhofer W, Muller E (2000) Molecular chaperones in the kidney: distribution, putative roles and regulation. Am J Physiol Renal Physiol 279:203-215
3. Bigas M, Durfort M, Poquet M (2006) Cytological response of hemocytes in the European flat oyster, Ostrea edulis, experimentally exposed to mercury. Biometals 19:659-673
4. Bolognesi C, Landini E, Roggieri P, Fabbri R, Viarengo A (1999) Genotoxicity biomarkers in the assessment of heavy metal effects in mussels: experimental studies. Environ Mol Mutagen 33:287-292
5. Booth S, Zeller D (2005) Mercury, food webs, and marine mammals: implications of diet and climate change for human health. Environ Health Perspect 113:521-526
6. Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of the protein-dye binding. Anal Biochem 72:248-254
7. Campbell LM, Norstrom RJ, Hobson KA, Muir DC, Backus S, Fisk AT (2005) Mercury and other trace elements in a pelagic Arctic marine food web (North Water Polynya, Baffin Bay). Sci Total Environ 351-352:247-263
8. Chabicovsky M, Niederstätter H, Thaler R, Hödl E, Parson W, Rossmanith W, Dallinger R (2003) Localization and quantification of CD- and CU-specific metallothionein isoform mRNA in cells and organs of the terrestrial gastropod helix pomatia. Toxicol Appl Pharm 190:25-36
9. Chang YT, Jong KJ, Liao BK, Wu SM (2007) Cloning and expression of metallothionein cDNA in the hard clam (Meretrix lusoria) upon cadmium exposure. Aquaculture 262:504-513
10. Cho YS, Choi BN, Kim KH, Kim SK, Kim DS, Bang IC, Nam YK (2006) Differential expression of Cu/Zn superoxide dismutase mRNA during exposures to heavy metals in rockbream (Oplegnathus fasciatus). Aquaculture 253:667-679
11. Company R, Serafim A, Bebianno MJ, Cosson R, Shillito B, Fiala-Medioni A (2004) Effect of cadmium, copper and mercury on antioxidant enzyme activities and lipid peroxidation in the gills of the hydrothermal vent mussel Bathymodiolus azoricus. Mar Environ Res 58:377-381
12. Davis SR, Cousins RJ (2000) Metallothionein expression in animals: a physiological perspective on function. J Nutr 130:1085-1088
13. Decataldo A, Di Leo A, Giandomenico S, Cardellicchio N (2004) Association of metals (mercury, cadmium and zinc) with metallothionein-like proteins in storage organs of stranded dolphins from the Mediterranean sea (Southern Italy). J Environ Monit 6:361-367
14. Desclaux-Marchand C, Paul-Pont I, Gonzalez P, Baudrimont M, de Montaudouin X (2007) Metallothionein gene identification and expression in the cockle (Cerastoderma edule) under parasitism (Trematodes) and cadmium contaminations. Aquat Living Resour 20:43-49
15. Dondero F, Piacentini L, Banni M, Rebelo M, Burlando B, Viarengo A (2005) Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation. Gene 345:259-270
16. Fang Y, Yang H, Wang T, Liu B, Zhao H, Chen M (2010) Metallothionein and superoxide dismutase responses to sublethal cadmium exposure in the clam Mactra veneriformis. Comp Biochem Physiol C 151:325-333
17. Faria M, Carrasco L, Diez S, Riva MC, Bayona JM, Barata C (2009) Multi-biomarker responses in the freshwater mussel Dreissena polymorpha exposed to polychlorobiphenyls and metals. Comp Biochem Physiol 149:281-288
18. Faria M, Huertas D, Soto DX, Grimalt JO, Catalan J, Riva MC, Barata C (2010) Contaminant accumulation and multi-biomarker responses in field collected zebra mussels (Dreissena polymorpha) and crayfish (Procambarus clarkii), to evaluate toxicological effects of industrial hazardous dumps in the Ebro river (NE Spain). Chemosphere 78:232-240
19. Frenzilli G, Bocchetti R, Pagliarecci M, Nigro M, Annarumma F, Scarcelli V, Fattorini D, Regoli F (2004) Time-course evaluation of ROS-mediated toxicity in mussels, Mytilus galloprovincialis, during a field translocation experiment. Mar Environ Res 58: 609-613
20. Fridovich I (1975) Superoxide dismutases. Annu Rev Biochem 44:147-159
21. Géret F, Jouan A, Turpin V, Bebianno MJ, Cosson RP (2002) Influence of metal exposure on metallothionein synthesis and lipid peroxidation in two bivalve mollusks: the oyster (Crassostrea gigas) and the mussel (Mytilus edulis). Aquat Living Resour 15:61-66
22. Gregory MA, Marshall DJ, George RC, Anandraj A, McClurg TP (2002) Correlations between metal uptake in the soft tissue of Perna perna and gill filament pathology after exposure to mercury. Mar Pollut Bull 45:114-125
23. Halliwell B, Gutteridge JMC (1985) The importance of free-radicals and catalytic metal-ions in human diseases. Mol Aspects Med 8:89-193
24. Hardivillier Y, Denis F, Demattei MV, Bustamante P, Laulier M, Cosson R (2006) Metal influence on metallothionein synthesis in the hydrothermal vent mussel Bathymodiolus thermophilus. Comp Biochem Physiol C 143:321-332
25. IPCS (1976) Environment health criteria 1: mercury. WHO, Geneva
26. IPCS (1991) Environment health criteria 118: inorganic mercury. WHO, Geneva
27. Jackson TA (1998) Mercury in aquatic ecosystems. In: Langston WJ, Bebianno MJ (eds) Metal metabolism in aquatic environments. Chapman and Hall, London, pp 77-157
28. Jo PG, Choi YK, Choi CY (2008) Cloning and mRNA expression of antioxidant enzymes in the Pacific oyster, Crassostrea gigas in response to cadmium exposure. Comp Biochem Physiol C 147:460-469
29. Kumagai YO, Mizukado S, Nagafune N, Shinyashik M, Homma-Yakeda S, Shimojo N (1997) Post-transcriptional elevation of mouse brain Mn-SOD protein by mercuric chloride. Brain Res 796:178-182
30. Lemoine S, Bigot Y, Sellos D, Cosson RP, Laulier M (2000) Metallothionein isoforms in Mytilus edulis (mollusca, bivalvia): complementary DNA characterization and quantification of expression in different organs after exposure to cadmium, zinc, and copper. Mar Biotechnol 2:195-203
31. Liang LN, Shi JB, He B, Jiang GB, Yuan CG (2003) Investigation of methylmercury and total mercury contamination in mollusk samples collected from coastal sites along the Chinese Bohai sea. J Agric Food Chem 51:7373-7378
32. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using realtime quantitative PCR and the 2(-Delta Delta C(T)) method. Methods: 25:402-408
33. 2 production and lipid peroxidation in vitro in rat kidney mitochondria. Biochem Pharmacol 42:S181-S187
34. MacDougal KC, Johnson MD, Burnett KG (1996) Exposure to mercury alters early activation events in fish leukocytes. Environ Health Perspect 104:1102-1106
35. Mahmood K, Yang JS, Chen DF, Wang MR, Yang F, Yang WJ (2009) Response of metallothionein gene-1 to laboratory exposure to heavy metals and thermal stress in the freshwater prawn Macrobrachium rosenbergii. J Hazard Mater 167:523-530
36. Marigómez I, Soto M, Cajaraville MP, Angulo E, Giamberini L (2002) Cellular and subcellular distribution of metals in molluscs. Micros Res Tech 56:358-392
37. Metian M, Warnau M, Cosson RP, Oberhänsli F, Bustamante P (2008) Bioaccumulation and detoxification processes of hg in the king scallop Pecten maximus: Field and laboratory investigations. Aquat Toxicol 90:204-213
38. Moore MN, Allen JI (2002) A computational model of the digestive gland epithelial cell of marine mussels and its simulated responses to oil-derived aromatic hydrocarbons. Mar Environ Res 54:579-584
39. Mourgaud Y, Martinez E, Geffard A, Andral B, Stanisière JY, Amiard JC (2002) Metallothionein concentration in the mussel Mytilus galloprovincialis as a biomarker of response to metal contamination: validation in the field. Biomarkers 7:479-490
40. Navarro A, Quirós L, Casado M, Faria M, Carrasco L, Benejam L, Benito J, Díez S, Raldúa D, Barata C, Bayona JM, Piña B (2009) Physiological responses to mercury in feral carp populations inhabiting the low Ebro river (NE Spain), a historically contaminated site. Aquat Toxicol 93:150-157
41. Ni DJ, Song LS, Gao Q, Wu LT, Yu YD, Zhao JM, Qiu LM, Zhang H, Shi FF (2007) The cDNA cloning and mRNA expression of cytoplasmic Cu, Zn superoxide dismutase (SOD) gene in scallop Chlamys farreri. Fish Shellfish Immunol 23:1032-1042
42. Oyanagui Y (1984) Reevaluation of assay-methods and establishment of kit for superoxide dismutase activity. Anal Biochem 142: 290-296
43. Pourahmad M, O'Brien PJ, Jokar F, Daraei B (2003) Carcinogenic metal induced sites of reactive oxygen species formation in hepatocytes. Toxicol In Vitro 17:803-810
44. Scheuhammer AM, Cherian MG (1986) Quantification of metallothioneins by a silver-saturation method. Toxicol Appl Pharmacol 82:417-425
45. Sener G, Sehirli AO, Ayanoglu-Dulger G (2003) Melatonin protects against mercury (II)-induced oxidative tissue damage in rats. Pharmacol Toxicol 93:290-296
46. Soazig L, Marc L (2003) Potential use of the levels of the mRNA of a specific metallothionein isoform (MT-20) in mussel (Mytilus edulis) as a biomarker of cadmium contamination. Mar Pollut Bull 46:1450-1455
47. Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497-500
48. Verlecar XN, Jena KB, Chainy GBN (2008) Modulation of antioxidant defences in digestive gland of Perna viridis (L.), on mercury exposures. Chemosphere 71:1977-1985
49. Vieira LR, Gravato C, Soares A, Morgado F, Guilhermino L (2009) Acute effects of copper and mercury on the estuarine fish Pomatoschistus microps: linking biomarkers to behaviour. Chemosphere 76:1416-1427
50. Wang CY, Wang XL (2007) Spatial distribution of dissolved Pb, Hg, Cd, Cu and as in the Bohai sea. J Environ Sci 19:1061-1066
51. Wang YW, Liang L, Shi JB, Jiang GB (2005) Study on the contamination of heavy metals and their correlations in mollusks collected from coastal sites along the Chinese Bohai Sea. Environ Int 31:1103-1113
52. Wang XY (2009) Physiological response of Mactra Veneriformis under cadmium and mercury pollution stress. Dissertation, Chinese Academy of Sciences
53. Wang SF, Jia YF, Wang SY, Wang X, Wang H, Zhao ZX, Liu BZ (2009a) Total mercury and monomethylmercury in water, sediments, and hydrophytes from the rivers, estuary, and bay along the Bohai sea coast, northeastern China. Appl Geochem 24:1702-1711
54. 2+ to the clam Mactra veneriformis. Mar Sci 12: 24-39 (in Chinese with English abstract)
55. Znidaric MT, Falnoga I, Skreblin M, Turk V (2006) Induction of metallothionein-like proteins by mercury and distribution of mercury and selenium in the cells of hepatopancreas and gill tissues in mussel Mytilus galloprovincialis. Biol Trace Elem Res 111:121-135
56. Zorita I, Bilbao E, Schad A, Cancio I, Soto M, Cajaraville MP (2007) Tissue- and cell-specific expression of metallothionein genes in cadmium- and copper-exposed mussels analyzed by in situ hybridization and RT-PCR. Toxicol Appl Pharmacol 220: 186-196