RNA quantification using noble metal nanoprobes: Simultaneous identification of several different mrna targets using color multiplexing and application to cancer diagnostics

Methods in Molecular Biology

Volumn 906, Issue , 2012, Pages 71-87

  Conde, João ^a,b   Doria, Gonçalo ^a   De La Fuente, Jesus M ^b   Baptista, Pedro Viana ^a  

^a UNIVERSIDADE NOVA DE LISBOA   (Portugal)

^b UNIVERSITY OF ZARAGOZA   (Spain)

Author keywords

Cancer; Colorimetric method; Gene expression; Gold nanoparticles; Gold:silver alloy nanoparticles; mRNA detection; Nanoprobes

Indexed keywords

GOLD; GOLD ALLOY; MESSENGER RNA; METAL NANOPARTICLE; SILVER;

ARTICLE; CHEMISTRY; CHRONIC MYELOID LEUKEMIA; COLORIMETRY; GENETIC SCREENING; GENETICS; HUMAN; METHODOLOGY; NANOTECHNOLOGY; SYNTHESIS;

COLORIMETRY; GENETIC TESTING; GOLD; GOLD ALLOYS; HUMANS; LEUKEMIA, MYELOGENOUS, CHRONIC, BCR-ABL POSITIVE; METAL NANOPARTICLES; NANOTECHNOLOGY; RNA, MESSENGER; SILVER;

EID: 84864856777     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-953-2_6     Document Type: Article

References (29)

1. Bustin SA (2000) Absolute quanti fi cation of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169-193
2. Weis JH et al (1992) Detection of rare mRNAs via quantitative RT-PCR. Trends Genet 8:263-264
3. Freeman WM, Walker SJ, Vrana KE (1999) Quantitative RT-PCR: pitfalls and potential. Biotechniques 26:112-115
4. http://nano.cancer.gov/about/plan/Accessed 11 May 2012
5. Baptista P et al (2008) Gold nanoparticles for the development of clinical diagnosis methods. Anal Bioanal Chem 391:943-950
6. Sato K, Hosokawa K, Maeda M (2005) Noncross-linking gold nanoparticle aggregation as a detection method for single-base substitutions. Nucleic Acids Res 33:e4
7. Mirkin CA et al (1996) A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature 382:607-609 (Pubitemid 26268949)
8. Taton TA, Mirkin CA, Letsinger RL (2000) Scanometric DNA array detection with nanoparticle probes. Science 289:1757-1760
9. Qin WJ, Yung LY (2007) Nanoparticle-based detection and quanti fi cation of DNA with single nucleotide polymorphism (SNP) discrimination selectivity. Nucleic Acids Res 35:e111
10. Elghanian R et al (1997) Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. Science 277:1078-1081 (Pubitemid 27371624)
11. Sato K, Hosokawa K, Maeda M (2003) Rapid aggregation of gold nanoparticles induced by non-cross-linking DNA hybridization. J Am Chem Soc 125:8102-8103 (Pubitemid 36828566)
12. Storhoff JJ et al (2004) Homogeneous detection of unampli fi ed genomic DNA sequences based on colorimetric scatter of gold nanoparticle probes. Nat Biotechnol 22:883-887 (Pubitemid 38886609)
13. Wilcoxon J (2009) Optical absorption properties of dispersed gold and silver alloy nanoparticles. J Phys Chem B 113:2647-2656
14. Liz-Marzan LM (2006) Tailoring surface plasmons through the morphology and assembly of metal nanoparticles. Langmuir 22:32-41 (Pubitemid 43063520)
15. Baptista P et al (2005) Colorimetric detection of eukaryotic gene expression with DNAderivatized gold nanoparticles. J Biotechnol 119:111-117 (Pubitemid 41219540)
16. Doria G, Franco R, Baptista P (2007) Nanodiagnostics: fast colorimetric method for single nucleotide polymorphism/mutation detection. IET Nanobiotechnol 1:53-57 (Pubitemid 47209800)
17. Conde J, de la Fuente JM, Baptista PV (2010) RNA quanti fi cation using gold nanoprobes-application to cancer diagnostics. J Nanobiotechnology 8:5
18. Veigas B et al (2010) Au-nanoprobes for detection of SNPs associated with antibiotic resistance in Mycobacterium tuberculosis . Nanotechnology 21:415101
19. Baptista PV et al (2006) Gold-nanoparticleprobe-based assay for rapid and direct detection of Mycobacterium tuberculosis DNA in clinical samples. Clin Chem 52:1433-1434 (Pubitemid 43956043)
20. Costa P et al (2010) Gold nanoprobes assay for identi fi cation of mycobacteria from the Mycobacterium tuberculosis complex. Clin Microbiol Infect 16:1464-1469
21. Doria G et al (2010) Gold-silver-alloy nanoprobes for one-pot multiplex DNA detection. Nanotechnology 21:255101
22. Hehlmann R, Hochhaus A, Baccarani M (2007) Chronic myeloid leukaemia. Lancet 370:342-350 (Pubitemid 47094995)
23. Shet AS, Jahagirdar BN, Verfaillie CM (2002) Chronic myelogenous leukemia: mechanisms underlying disease progression. Leukemia 16: 1402-1411
24. Ren R (2005) Mechanisms of BCR-ABL in the pathogenesis of chronic myelogenous leukaemia. Nat Rev Cancer 5:172-183 (Pubitemid 40314949)
25. Melo JV, Hughes TP, Apperley JF (2003) Chronic myeloid leukemia. Hematology Am Soc Hematol Educ Program 132-152
26. Chomczynski P, Sacchi N (1987) Single step method of RNA isolation by acid guanidinum thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159 (Pubitemid 17064313)
27. Chomczynski P (1993) A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15:532-537 (Pubitemid 23270488)
28. Doria G et al (2010) Optimizing Au-nanoprobes for speci fi c sequence discrimination. Colloids Surf B 77:122-124
29. Parsons PW, Estrada FJ (1942) Changes in volume on mixing solutions. Ind Eng Chem 34:949-952