Evaluation of PCR for cutaneous leishmaniasis diagnosis and species identification using filter paper samples in Panama, Central America

Transactions of the Royal Society of Tropical Medicine and Hygiene

Volumn 106, Issue 9, 2012, Pages 544-548

  Miranda, A ^a   Saldana A ^a,b   Gonzalez K ^a   Paz, H ^a   Santamaria G ^c   Samudio, F ^a   Calzada, J E ^a,b  

^a Instituto Conmemorativo Gorgas de Estudios de la Salud (ICGES)   (Panama)

^b UNIVERSIDAD DE PANAMÁ   (Panama)

^c Hospital Dr Luis Fábrega   (Panama)

Author keywords

Diagnosis; Filter paper; Leishmania panamensis; Leishmaniasis; Panama; PCR

Indexed keywords

ADOLESCENT; ADULT; AGED; ARTICLE; CENTRAL AMERICA; CHILD; CONTROLLED STUDY; DIAGNOSTIC VALUE; DNA EXTRACTION; FEMALE; FILTER; FILTER PAPER; HUMAN; IN VITRO STUDY; INFANT; LEISHMANIA; MAJOR CLINICAL STUDY; MALE; NONHUMAN; PAPER; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; ROOM TEMPERATURE; SCHOOL CHILD; SENSITIVITY AND SPECIFICITY; SKIN LEISHMANIASIS;

DNA; FEMALE; FILTRATION; HUMANS; LEISHMANIASIS, CUTANEOUS; MALE; PANAMA; PAPER; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SKIN; SPECIES SPECIFICITY; SPECIMEN HANDLING;

LEISHMANIA PANAMENSIS;

EID: 84864795362     PISSN: 00359203     EISSN: 18783503     Source Type: Journal    
DOI: 10.1016/j.trstmh.2012.05.005     Document Type: Article

References (22)

1. WHO Tropical Diseases Research Scientific Working Group. Report on leishmaniasis. 2-4 February 2004. Geneva: World Health Organization; 2004. TDR/SWG/04.
2. Bern C., Maguire J.H., Alvar J. Complexities of assessing the disease burden attributable to leishmaniasis. PLoS Negl Trop Dis 2008, 2:e313.
3. WHO. Control of the leishmaniasis: report of a meeting of the WHO Expert Committee on the Control of Leishmaniases, Geneva, 22-26 March 2010. Geneva: World Health Organization; 2011. Technical Report Series No. 949.
4. Reithinger R., Dujardin J.C., Louzir H., Pirmez C., Alexander B., Brooker S. Cutaneous leishmaniasis. Lancet Infect Dis 2007, 7:581-596.
5. Miranda A., Carrasco R., Paz H., et al. Molecular epidemiology of American tegumentary leishmaniasis in Panama. Am J Trop Med Hyg 2009, 81:565-571.
6. Rodríguez-Cortés A., Ojeda A., Francino O., López-Fuertes L., Timón M., Alberola J. Leishmania infection: laboratory diagnosing in the absence of a " gold standard" . Am J Trop Med Hyg 2010, 82:251-256.
7. Vega-López F. Diagnosis of cutaneous leishmaniasis. Curr Opin Infect Dis 2003, 16:97-101.
8. Reithinger R., Dujardin J.C. Molecular diagnosis of leishmaniasis: current status and future applications. J Clin Microbiol 2007, 45:21-25.
9. Belli A., Rodriguez B., Aviles H., Harris E. Simplified polymerase chain reaction detection of new world Leishmania in clinical specimens of cutaneous leishmaniasis. Am J Trop Med Hyg 1998, 58:102-109.
10. Vergel C., Walker J., Saravia N.G. Amplification of human DNA by primers targeted to Leishmania kinetoplast DNA and post-genome considerations in the detection of parasites by a polymerase chain reaction. Am J Trop Med Hyg 2005, 72:423-429.
11. Fagundes A., Schubach A., Paula C.C., et al. Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre. Mem Inst Oswaldo Cruz 2010, 105:109-112.
12. Bensoussan E., Nasereddin A., Jonas F., Schnur L.F., Jaffe C.L. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J Clin Microbiol 2006, 44:1435-1439.
13. Shahbazi F., Shahabi S., Kazemi B., Mohebali M., Abadi A.R., Zare Z. Evaluation of PCR assay in diagnosis and identification of cutaneous leishmaniasis: a comparison with the parasitological methods. Parasitol Res 2008, 103:1159-1162.
14. de Bruijn M.H., Barker D.C. Diagnosis of New World leishmaniasis: specific detection of species of the Leishmania braziliensis complex by amplification of kinetoplast DNA. Acta Trop 1992, 52:45-58.
15. Fuehrer H.P., Fally M.A., Habler V.E., Starzengruber P., Swoboda P., Noedl H. Novel nested direct PCR technique for malaria diagnosis using filter paper samples. J Clin Microbiol 2011, 49:1628-1630.
16. Marques M.J., Volpini A.C., Genaro O., Mayrink W., Romanha A.J. Simple form of clinical sample preservation and Leishmania DNA extraction from human lesions for diagnosis of American cutaneous leishmaniasis via polymerase chain reaction. Am J Trop Med Hyg 2001, 65:902-906.
17. Boggild A.K., Valencia B.M., Espinosa D., et al. Detection and species identification of Leishmania DNA from filter paper lesion impressions for patients with American cutaneous leishmaniasis. Clin Infect Dis 2010, 50:e1-e6.
18. Romero G.A., Noronha E.F., Pirmez C., et al. Sensitivity and reproducibility of a PCR assay for Leishmania detection using skin biopsy imprints on filter paper. Acta Trop 2009, 109:74-77.
19. Jamjoom M., Sultan A.H. Diagnosis of clinical samples spotted on FTA cards using PCR-based methods. J Egypt Soc Parasitol 2009, 39:227-246.
20. Christensen H.A., Herrer A., Telford S.R. Enzootic cutaneous leishmaniasis in eastern Panama. II. Entomological investigations. Ann Trop Med Parasitol 1972, 66:55-66.
21. Christensen H.A., Fairchild G.B., Herrer A., Johnson C.M., Young D.G., de Vásquez A.M. The ecology of cutaneous leishmaniasis in the Republic of Panama. J Med Entomol 1983, 20:463-484.
22. Christensen H.A., de Vasquez A.M., Petersen J.L. Short report: epidemiologic studies on cutaneous leishmaniasis in eastern Panama. Am J Trop Med Hyg 1999, 60:54-57.