In vitro assays measuring protection by proteins such as cystatin c of primary cortical neuronal and smooth muscle cells

Methods in Molecular Biology

Volumn 849, Issue , 2012, Pages 275-287

  Gauthier, Sebastien A ^a,b   Tizon, Belen ^a,b   Sahoo, Susmita ^a,b   Levy, Efrat ^a,b  

^a NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b NATHAN S KLINE INSTITUTE FOR PSYCHIATRIC RESEARCH   (United States)

Author keywords

Amyloid ; Cell death; Cell survival; Cerebrovascular cells; Cortical neurons; Cystatin C; Primary cell culture; Protection assays; Stress induced cytotoxicity

Indexed keywords

CYSTATIN C; 3',6' DIACETYLFLUORESCEIN; 3',6'-DIACETYLFLUORESCEIN; FLUORESCEIN DERIVATIVE; PROPIDIUM IODIDE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN CELL; CELL COUNT; CELL CULTURE; CELL DEATH; CELL DIVISION; CELL GROWTH; CELL PROLIFERATION; CELL PROTECTION; CELL STRESS; CELL SURVIVAL; CELL SUSPENSION; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; GENE INACTIVATION; IN VITRO STUDY; MOUSE; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; SMOOTH MUSCLE FIBER; ANIMAL; BRAIN CORTEX; CULTURE TECHNIQUE; CYTOLOGY; EXTRACELLULAR SPACE; GENETICS; METABOLISM; METHODOLOGY; NERVE CELL; STAINING;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIVISION; CELL PROLIFERATION; CELL SURVIVAL; CEREBRAL CORTEX; CYSTATIN C; CYTOLOGICAL TECHNIQUES; EXTRACELLULAR SPACE; FLUORESCEINS; GENE KNOCKOUT TECHNIQUES; MICE; MYOCYTES, SMOOTH MUSCLE; NEURONS; OXIDATIVE STRESS; PROPIDIUM; STAINING AND LABELING;

EID: 84864624807     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-551-0_19     Document Type: Article

References (16)

1. Sastre, M., Calero, M., Pawlik, M., Mathews, P. M., Kumar, A., Danilov, V., Schmidt, S. D., Nixon, R. A., Frangione, B., and Levy, E. (2004) Binding of cystatin C to Alzheimer's amyloid b inhibits amyloid fi bril formation. Neurobiol Aging 25 , 1033-1043
2. Kaeser, S. A., Herzig, M. C., Coomaraswamy, J., Kilger, E., Selenica, M. L., Winkler, D. T., Staufenbiel, M., Levy, E., Grubb, A., and Jucker, M. (2007) Cystatin C modulates cerebral b-amyloidosis. Nat Genet 39 , 1437-1439
3. Mi, W., Pawlik, M., Sastre, M., Jung, S. S., Radvinsky, D. S., Klein, A. M., Sommer, J., Schmidt, S. D., Nixon, R. A., Mathews, P. M., and Levy, E. (2007) Cystatin C inhibits amyloid-b deposition in Alzheimer's disease mouse models. Nat Genet 39 , 1440-1442
4. Selenica, M. L., Wang, X., Ostergaard-Pedersen, L., Westlind-Danielsson, A., and Grubb, A. (2007) Cystatin C reduces the in vitro formation of soluble A b 1-42 oligomers and protofi-brils. Scand J Clin Lab Invest 67 , 179-190
5. Tizon, B., Ribe, E. M., Mi, W., Troy, C. M., and Levy, E. (2010) Cystatin C protects neuronal cells from amyloid b-induced toxicity. J Alzheimers Dis 19 , 665-894
6. Tizon, B., Sahoo, S., Yu, H., Gauthier, S., Kumar, A. R., Mohan, P., Figliola, M., Pawlik, M., Grubb, A., Uchiyama, Y., Bandyopadhyay, S., Cuervo, A. M., Nixon, R. A., and Levy, E. (2010) Induction of autophagy by cystatin C: A mechanism that protects murine primary cortical neurons and neuronal cell lines. PLoS One 5 , e9819
7. Deng, A., Irizarry, M. C., Nitsch, R. M., Growdon, J. H., and Rebeck, G. W. (2001) Elevation of cystatin C in susceptible neurons in alzheimer's disease. Am J Pathol 159 , 1061-1068
8. Palm, D. E., Knuckey, N. W., Primiano, M. J., Spangenberger, A. G., and Johanson, C. E. (1995) Cystatin C, a protease inhibitor, in degenerating rat hippocampal neurons following transient forebrain ischemia. Brain Res 691 , 1-8
9. Pirttila, T. J., and Pitkanen, A. (2006) Cystatin C expression is increased in the hippocampus following photothrombotic stroke in rat. Neurosci Lett 395 , 108-113
10. Huh, C. G., Hakansson, K., Nathanson, C. M., Thorgeirsson, U. P., Jonsson, N., Grubb, A., Abrahamson, M., and Karlsson, S. (1999) Decreased metastatic spread in mice homozygous for a null allele of the cystatin C protease inhibitor gene. Mol Pathol 52 , 332-340
11. Pawlik, M., Sastre, M., Calero, M., Mathews, P. M., Schmidt, S. D., Nixon, R. A., and Levy, E. (2004) Overexpression of human cystatin C in transgenic mice does not affect levels of endogenous brain amyloid b peptide. J Mol Neurosci 22 , 13-18
12. Abrahamson, M., Barrett, A. J., Salvesen, G., and Grubb, A. O. (1986) Isolation of six cysteine proteinase inhibitors from human urine. Their physicochemical and enzyme kinetic properties and concentrations in biological fl uids. J Biol Chem 261 , 11282-11289
13. Yamada, T., Mukaiyama, I., Miyake, N., and Igari, J. (2002) Measurement of cystatin C in cerebrospinal fl uid. Rinsho Byori 50 , 613-617
14. Jones, K. H., and Senft, J. A. (1985) An improved method to determine cell viability by simultaneous staining with fl uorescein diacetate-propidium iodide. J Histochem Cytochem 33 , 77-79
15. Soames, A. R., Lavender, D., Foster, J. R., Williams, S. M., and Wheeldon, E. B. (1994) Image analysis of bromodeoxyuridine (BrdU) staining for measurement of S-phase in rat and mouse liver. J Histochem Cytochem 42 , 939-944
16. Perry, S., Norman, J., Litzburg, A., and Gelbard, H. (2004) Antioxidants are required during the early critical period, but not later, for neuronal survival. J Neurosci Res 78 , 485-492.