Impact of each individual component of the mutated PTS Nag on glucose uptake and phosphorylation in Ralstonia eutropha G +1

Applied Microbiology and Biotechnology

Volumn 95, Issue 3, 2012, Pages 735-744

  Raberg, Matthias ^a   Kaddor, Chlud ^a   Kusian, Bernhard ^b   Stahlhut, Gertrud ^b   Budinova, Radka ^b   Kolev, Nikola ^b   Bowien, Botho ^b   Steinbüchel, Alexander ^a,c  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b UNIVERSITY OF GÖTTINGEN   (Germany)

^c KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

Author keywords

Carbohydrate phosphotransferase system (PTS); Glucose utilization; Nag operon; PHB; Ralstonia eutropha

Indexed keywords

CYTOSOLIC; CYTOSOLIC DOMAINS; DE-REPRESSION; DELETION MUTANTS; GLUCOKINASE; GLUCOSE TRANSPORT; GLUCOSE UPTAKE; INDIVIDUAL COMPONENTS; MEMBRANE COMPONENTS; MEMBRANE SPANNING; NAG OPERON; PHB; PHOSPHOTRANSFERASE SYSTEM; POINT MUTATIONS; PROTEOMIC ANALYSIS; RALSTONIA EUTROPHA; SOLUBLE DOMAINS;

AMINO ACIDS; BACTERIA; CARBOHYDRATES; CELL MEMBRANES; CYTOLOGY; PHOSPHORYLATION; PROTEINS;

GLUCOSE;

GLUCOSE; N ACETYLGLUCOSAMINE SPECIFIC PHOSPHOTRANSFERASE; PHOSPHOTRANSFERASE; THREONINE; UNCLASSIFIED DRUG;

BACTERIUM; BIOLOGICAL UPTAKE; ENZYME ACTIVITY; GLUCOSE; MEMBRANE; MUTATION; PHOSPHORITE; PROTEOMICS; ULTRAVIOLET RADIATION;

ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; CUPRIAVIDUS NECATOR; GENE DELETION; GENE MUTATION; GLUCOSE TRANSPORT; MISSENSE MUTATION; MUTANT; NONHUMAN; OPERON; PROTEIN PHOSPHORYLATION; SUBSTITUTION REACTION;

ACETYLGLUCOSAMINE; CUPRIAVIDUS NECATOR; GENE DELETION; GLUCOSE; MUTATION, MISSENSE; PHOSPHORYLATION; PHOSPHOTRANSFERASES;

CUPRIAVIDUS NECATOR;

EID: 84864593380     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-3911-9     Document Type: Article

References (46)

1. Aragno M, Schlegel HG (1992) The prokaryotes. The mesophilic hydrogen-oxidizing (Knallgas) bacteria. Springer, New York, pp 344-384
2. Bairoch A (1991) PROSITE: a dictionary of sites and patterns in proteins. Nucleic Acids Res 19:2241-2245
3. Begley GS, Hansen DE, Jacobson GR, Knowles JR (1982) Stereochemical course of the reactions catalyzed by the bacterial phosphoenolpyruvate: glucose phosphotransferase system. Biochemistry 21:5552-5556
4. Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acid Res 7:1513-1523
5. Bowien B, Schlegel HG (1981) Physiology and biochemistry of aerobic hydrogen-oxidizing bacteria. Annu Rev Microbiol 35:405-452
6. Brandl H, Gross RA, Lenz RW, Fuller RC (1988) Pseudomonas oleovorans as a source of poly(ß-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl Environ Microbiol 66:2117-2124
7. Brückner R, Titgemeyer F (2002) Carbon catabolite repression in bacteria: choice of the carbon source and autoregulatory limitation of sugar utilization. FEMS Microbiol Lett 209:141-148
8. Burstein C, Cohn M, Kepes A, Monod J (1965) Role of lactose and its metabolic products in the induction of the lactose operon in Escherichia coli. Biochim Biophys Acta 95:634-639
9. Cai M, Williams DC Jr., Wang G, Lee BR, Peterkofsky A, Clore GM (2003) Solution structure of the phosphoryl transfer complex between the signal transducing protein IIAGlucose and the cytoplasmic domain of the glucose transporter IICBGlucose of the Escherichia coli glucose phosphotransferase system. J Biol Chem 278:25191-25206
10. Chen Y, Reizer J, Saier MH Jr., Fairbrother WJ, Wright PE (1993) Mapping of the binding interfaces of the proteins of the bacterial phosphotransferase system, HPr and IIAglc. Biochemistry 32:32-37
11. Deutscher J, Franke C, Postma PW (2006) How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria. Microbiol Mol Biol Rev 70:939-1031
12. Erni B (1986) Glucose-specific permease of the bacterial phosphotransferase system: phosphorylation and oligomeric structure of the glucose-specific IIGlc-IIIGlc complex of Salmonella typhimurium. Biochemistry 25:305-312
13. Friedrich CG, Bowien B, Friedrich B (1979) Formate and oxalate metabolism in Alcaligenes eutrophus. J Gen Microbiol 115:185-192
14. Garrett DS, Seok YJ, Peterkofsky A, Gronenborn AM, Clore GM (1999) Solution structure of the 40,000 Mr phosphoryl transfer complex between the N-terminal domain of enzyme I and HPr. Nat Struct Biol 6:166-173
15. Gemmecker G, Eberstadt M, Buhr A, Lanz R, Grdadolnik SG, Kessler H, Erni B (1997) Glucose transporter of Escherichia coli: NMR characterization of the phosphocysteine form of the IIBGlc domain and its binding interface with the IIAGlc subunit. Biochemistry 36:7408-7417
16. Gottschalk G (1964) Verwertung von Fructose durch Hydrogenomonas H16. Arch Mikrobiol 67:51-57
17. Gottschalk G, Eberhardt U, Schlegel HG (1964) Verwertung von Fructose durch Hydrogenomonas H16. Arch Mikrobiol 48:95-108
18. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95-98
19. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557-580
20. Hogrefe C, Römermann D, Friedrich B (1981) Alcaligenes eutrophus hydrogenase genes (Hox). J Bacteriol 158:43-48
21. Jeffke T, Gropp NH, Kaiser C, Grzeszik C, Kusian B, Bowien B (1999) Mutational analysis of the cbb Operon (CO2 assimilation) promotor of Ralstonia eutropha. J Bacteriol 14:4374-4380
22. Kaddor C, Steinbüchel A (2011) Effects of PEP-PTS homologous proteins on carbohydrate uptake and PHB accumulation in Ralstonia eutropha H16. Appl Environ Microbiol 77:3582-3590
23. Kersters K, De Ley J (1984) Genus Alcaligenes Castellani and Chalmers 1919. In: Krieg NR, Holt JG (eds) Bergey's manual of systematic bacteriology, vol 1. Williams and Wilkins, Baltimore, pp 361-373
24. König C (1967) Über die Kultur von Hydrogenomonas H16 im Chemostaten unter chemoorganotrophen Bedingungen. Dissertation, University of Göttingen
25. König C, Sammler I, Wilde E, Schlegel HG (1969) Konstitutive Glucose-6-phosphat-Dehydrogenase bei Glucose verwertenden Mutanten von einem kryptischen Wildstamm. Arch Mikrobiol 67:51-57
26. Krauße D, Hunold K, Kusian B, Lenz O, Stülke J, Bowien B, Deutscher J (2009) Essential role of the hprK gene in Ralstonia eutropha H16. J Mol Microbiol Biotechnol 17:146-152
27. Marmur J (1961) A procedure for the isolation of desoxyribonucleic acids from microorganisms. J Mol Biol 3:208-218
28. Meins M, Jenö P, Müller D, Richter WJ, Rosenbusch JR, Erni B (1993) Cysteine phosphorylation of the glucose transporter of Escherichia coli. J Biol Chem 268:11604-11609
29. Oh H, Park Y, Park C (1999) A mutated PtsG, the glucose transporter, allows uptake of D-ribose. J Biol Chem 274:14006-14011
30. Orita I, Iwazawa R, Nakamura S, Fukui T (2011) Identification of mutation points in Cupriavidus necator NCIMB 11599 and genetic reconstitution of glucose-utilization ability in wild strain H16 for polyhydroxyalkanoate production. J Biosci Bioeng. doi:10.1016/j.jbiosc.2011.09.014
31. Pohlmann A, Fricke WF, Reinecke F, Kusian B, Liesegang H, Cramm R, Eitinger T, Ewering C, Pötter M, Schwartz E, Strittmatter A, Voß I, Gottschalk G, Steinbüchel A, Friedrich B, Bowien B (2006) Hydrogen-based biotechnology: genome sequence of the bioplastic-producing "Knallgas" bacterium Ralstonia eutropha H16. Nature Biotechnol 24:1257-1262
32. Pötter M, Müller H, Steinbüchel A (2005) Influence of homologous phasins (PhaP) on PHA accumulation and regulation of their expression by the transcriptional repressor PhaR in Ralstonia eutropha H16. Microbiology (SGM) 151:825-833
33. Quandt J, Hynes MF (1993) Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria. Gene 127:15-21
34. Raberg M, Peplinski K, Heiss S, Ehrenreich A, Voigt B, Döring C, Bömeke M, Hecker M, Steinbüchel A (2011) Proteomic and transcriptomic elucidation of the mutant Ralstonia eutropha G+1 with regard to glucose utilization. Appl Environ Microbiol 77:2058-2070
35. Robillard GT, Broos J (1999) Structure/function studies on the bacter i a l carbohydrate transporters, enzymes I I , of the phosphoenolpyruvate-dependent phosphotransferase system. Biochim Biophys Acta 1422:73-104
36. Ruijter GJG, Postma PW, van Dam K (1991) Energetics of glucose uptake in a Salmonella typhimurium mutant containing uncoupled IIGlc. Arch Microbiol 155:234-237
37. Ruijter GJG, van Meurs G, Verwey MA, Postma PW, van Dam K (1992) Analysis of mutations that uncouple transport from phosphorylation in enzyme IIGlc of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system. J Bacteriol 174:2843-2850
38. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
39. Schlegel HG, Gottschalk G (1965) Verwertung von Glucose durch eine Mutante von Hydrogenomonas H16. Biochem Z 841:249-259
40. Schlegel HG, Trüper HG (1966) Repression of enzyme formation in Hydrogenomonas strain, H16G+ by molecular hydrogen and by fructose. Antonie v Leeuwenhoek 32:277-292
41. Schlegel HG, Kaltwasser H, Gottschalk G (1961) Ein Submersverfahren zur Kultur wasserstoffoxidierender Bakterien: Wachstumsphysiologische Untersuchungen. Arch Mikrobiol 38:209-222
42. Schultz J, Milpetz F, Bork P, Ponting CP (1998) SMART, a simple modular architecture research tool: identification of signaling domains. Proc Natl Acad Sci U S A 95:5857-5864
43. Siebold C, Flukiger K, Beutler R, Erni B (2001) Carbohydrate transporters of the bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS). FEBS Lett 504:104-111
44. Simon R, Priefer U, Pühler A (1983) Vector plasmids for in vivo and in vitro manipulations of Gram negative bacteria. In: Pühler A (ed) Molecular genetics of the bacteria-plant interaction. Springer, Berlin, pp 98-106
45. Timm A, Steinbüchel A (1990) Formation of polyesters consisting of medium-chain-length 3-hydroxyalkanoic acids from gluconate by Pseudomonas aeruginosa and other fluorescent pseudomonads. Appl Environ Microbiol 56:3360-3367
46. Wang GS, Louis JM, Sondej M, Seok YJ, Peterkofsky A, Clore GM (2000) Solution structure of the phosphoryl transfer complex between the signal transducing proteins HPr and IIAGlucose of the Escherichia coli phosphoenolpyruvate: sugar phosphotransferase system. EMBO J 19:5635-5649