Identification of mutation points in Cupriavidus necator NCIMB 11599 and genetic reconstitution of glucose-utilization ability in wild strain H16 for polyhydroxyalkanoate production

Journal of Bioscience and Bioengineering

Volumn 113, Issue 1, 2012, Pages 63-69

  Orita, Izumi ^a   Iwazawa, Reiko ^a   Nakamura, Satoshi ^a   Fukui, Toshiaki ^a  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Biodegradable plastic; Biomass plastic; Cupriavidus necator; Glucose utilizing mutant; Homologous recombination; Polyhydroxyalkanoate; Ralstonia eutropha

Indexed keywords

BIODEGRADABLE PLASTICS; CUPRIAVIDUS NECATOR; GLUCOSE-UTILIZING MUTANT; HOMOLOGOUS RECOMBINATION; POLY-HYDROXYALKANOATE; RALSTONIA EUTROPHA;

BACTERIA; BIODEGRADABLE POLYMERS; ECOLOGY; ENCODING (SYMBOLS); GENES; PHOSPHORYLATION;

GLUCOSE;

FRUCTOSE; GLUCOSE; POLYHYDROXYALKANOIC ACID;

ARTICLE; BACTERIAL GENE; CUPRIAVIDUS NECATOR; GENE EXPRESSION; GENE SEQUENCE; GLUCOSE TRANSPORT; GLUCOSE UTILIZATION; NONHUMAN; NONSENSE MUTATION; PHOSPHORYLATION; POINT MUTATION;

ANIMALS; BACTERIAL PROTEINS; CODON, NONSENSE; CUPRIAVIDUS NECATOR; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; GLUCOKINASE; GLUCOSE; HOMOLOGOUS RECOMBINATION; METABOLIC ENGINEERING; MUTATION, MISSENSE; PHOSPHOTRANSFERASES; POLYHYDROXYALKANOATES; ZYMOMONAS;

CUPRIAVIDUS NECATOR; ZYMOMONAS MOBILIS;

EID: 84855340725     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.09.014     Document Type: Article

References (38)

1. Ragauskas A.J., Williams C.K., Davison B.H., Britovsek G., Cairney J., Eckert C.A., Frederick W.J., Hallett J.P., Leak D.J., Liotta C.L., other 4 authors The path forward for biofuels and biomaterials. Science 2006, 311:484-489.
2. Madison L.L., Huisman G.W. Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol. Mol. Biol. Rev. 1999, 63:21-53.
3. Sudesh K., Doi Y. Molecular design and biosynthesis of biodegradable polyesters. Polym. Adv. Technol. 2000, 11:865-872.
4. Reinecke F., Steinbuchel A. Ralstonia eutropha strain H16 as model organism for PHA metabolism and for biotechnological production of technically interesting biopolymers. J. Mol. Microbiol. Biotechnol. 2009, 16:91-108.
5. Fukui T., Abe H., Doi Y. Engineering of Ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from fructose and solid-state properties of the copolymer. Biomacromolecules 2002, 3:618-624.
6. Fukui T., Doi Y. Efficient production of polyhydroxyalkanoates from plant oils by Alcaligenes eutrophus and its recombinant strain. Appl. Microbiol. Biotechnol. 1998, 49:333-336.
7. Fukui T., Suzuki M., Tsuge T., Nakamura S. Microbial synthesis of poly((R)-3-hydroxybutyrate-co-3-hydroxypropionate) from unrelated carbon sources by engineered Cupriavidus necator. Biomacromolecules 2009, 10:700-706.
8. Mifune J., Nakamura S., Fukui T. Targeted engineering of Cupriavidus necator chromosome for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from vegetable oil. Can. J. Chem. 2008, 86:621-627.
9. Mifune J., Nakamura S., Fukui T. Engineering of pha operon on Cupriavidus necator chromosome for efficient biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from vegetable oil. Polym. Degrad. Stab. 2010, 95:1305-1312.
10. Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Potter M., Schwartz E., other 6 authors Genome sequence of the bioplastic-producing "Knallgas" bacterium Ralstonia eutropha H16. Nat. Biotechnol. 2006, 24:1257-1262.
11. Baldwin S.A., Henderson P.J. Homologies between sugar transporters from eukaryotes and prokaryotes. Annu. Rev. Physiol. 1989, 51:459-471.
12. Postma P.W., Lengeler J.W., Jacobson G.R. Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. Microbiol. Rev. 1993, 57:543-594.
13. Sichwart S., Hetzler S., Broker D., Steinbuchel A. Extension of the substrate utilization range of Ralstonia eutropha strain H16 by metabolic engineering to include mannose and glucose. Appl. Environ. Microbiol. 2011, 77:1325-1334.
14. Kim H.-Y., Park J.-S., Shin H.-D., Lee Y.-H. Isolation of glucose utilizing mutant of Alcaligenes eutrophus, its substrate selectivity, and accumulation of Poly-β-hydroxybutyrate. J. Microbiol. 1995, 33:51-58.
15. Konig C., Sammler I., Wilde E., Schlegel H.G. Constitutive glucose-6-phosphate dehydrogenase in mutants utilizing glucose, which are derived from cryptic wildtype strains. Arch. Mikrobiol. 1969, 67:51-57.
16. Schlegel H.G., Gottschalk G. Verwertung von glucose durch eine mutante von Hydrogenomonas H16. Biochim. Z. 1965, 341:249-259.
17. Byrom D. Production of poly-β-hydroxybutyrate: poly-β-hydroxyvalerate copolymers. FEMS Microbiol. Lett. 1992, 103:247-250.
18. Kim B.S., Lee S.C., Lee S.Y., Chang H.N., Chang Y.K., Woo S.I. Production of poly(3-hydroxybutyric acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control. Biotechnol. Bioeng. 1994, 43:892-898.
19. Ryu H.W., Hahn S.K., Chang Y.K., Chang H.N. Production of poly(3-hydroxybutyrate) by high cell density fed-batch culture of Alcaligenes eutrophus with phospate limitation. Biotechnol. Bioeng. 1997, 55:28-32.
20. Shang L., Jiang M., Chang H.N. Poly(3-hydroxybutyrate) synthesis in fed-batch culture of Ralstonia eutropha with phosphate limitation under different glucose concentrations. Biotechnol. Lett. 2003, 25:1415-1419.
21. +1 with regard to glucose utilization. Appl. Environ. Microbiol. 2011, 77:2058-2070.
22. Simon R., Priefer U., Pühler A. A broad host range mobilization system for in vivo genetic engineering. Transposon mutagenesis in Gram negative bacteria. Biotechnology 1983, 1:784-791.
23. Fukui T., Doi Y. Cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of Aeromonas caviae. J. Bacteriol. 1997, 179:4821-4830.
24. Kovach M.E., Elzer P.H., Hill D.S., Robertson G.T., Farris M.A., Roop R.M., Peterson K.M. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 1995, 166:175-176.
25. de Lorenzo V., Herrero M., Jakubzik U., Timmis K.N. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative eubacteria. J. Bacteriol. 1990, 172:6568-6572.
26. BAD promoter. J. Bacteriol. 1995, 177:4121-4130.
27. Woodall C.A. Electroporation of E. coli. Methods Mol. Biol. 2003, 235:55-69.
28. Schafer A., Tauch A., Jager W., Kalinowski J., Thierbach G., Puhler A. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 1994, 145:69-73.
29. Friedrich B., Hogrefe C., Schlegel H.G. Naturally occurring genetic transfer of hydrogen-oxidizing ability between strains of Alcaligenes eutrophus. J. Bacteriol. 1981, 147:198-205.
30. Fukui T., Ohsawa K., Mifune J., Orita I., Nakamura S. Evaluation of promoters for gene expression in polyhydroxyalkanoate-producing Cupriavidus necator H16. Appl. Microbiol. Biotechnol. 2011, 89:1527-1536.
31. Deutscher J., Francke C., Postma P.W. How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria. Microbiol. Mol. Biol. Rev. 2006, 70:939-1031.
32. Hoskisson P.A., Rigali S. Chapter 1: Variation in form and function the helix-turn-helix regulators of the GntR superfamily. Adv. Appl. Microbiol. 2009, 69:1-22.
33. Rigali S., Derouaux A., Giannotta F., Dusart J. Subdivision of the helix-turn-helix GntR family of bacterial regulators in the FadR, HutC, MocR, and YtrA subfamilies. J. Biol. Chem. 2002, 277:12507-12515.
34. Byrom D. Polymer synthesis by microorganisms: technology and economics. Trends Biotechnol. 1987, 5:246-250.
35. Buhr A., Erni B. Membrane topology of the glucose transporter of Escherichia coli. J. Biol. Chem. 1993, 268:11599-11603.
36. Saraceni-Richards C.A., Jacobson G.R. A conserved glutamate residue, Glu-257, is important for substrate binding and transport by the Escherichia coli mannitol permease. J. Bacteriol. 1997, 179:1135-1142.
37. Rigali S., Nothaft H., Noens E.E., Schlicht M., Colson S., Muller M., Joris B., Koerten H.K., Hopwood D.A., Titgemeyer F., van Wezel G.P. The sugar phosphotransferase system of Streptomyces coelicolor is regulated by the GntR-family regulator DasR and links N-acetylglucosamine metabolism to the control of development. Mol. Microbiol. 2006, 61:1237-1251.
38. Aravind L., Anantharaman V. HutC/FarR-like bacterial transcription factors of the GntR family contain a small molecule-binding domain of the chorismate lyase fold. FEMS Microbiol. Lett. 2003, 222:17-23.