The effect of hyperosmolar stimuli and cyclophosphamide on the culture of normal rat urothelial cells in vitro

Cellular and Molecular Biology Letters

Volumn 17, Issue 2, 2012, Pages 196-205

  Juszczak, Kajetan ^a,b   Kaszuba Zwoińska, Jolanta ^a   Chorobik, Paulina ^a   Ziomber, Agata ^a   Thor, Piotr Jan ^a  

^a JAGIELLONIAN UNIVERSITY   (Poland)

^b Rydygier's Memorial Hospital   (Poland)

Author keywords

Apoptosis; Bladder; Culture; Cyclophosphamide; Hyperosmolarity; Necrosis; Overactive bladder; Rat; Urothelial cell

Indexed keywords

CYCLOPHOSPHAMIDE; LIPOCORTIN 5; PROPIDIUM IODIDE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CULTURE; CELL DEATH; CELL STIMULATION; CELL VIABILITY; CONTROLLED STUDY; DRUG CYTOTOXICITY; DRUG EFFECT; FEMALE; FLOW CYTOMETRY; HYPEROSMOLARITY; IN VITRO STUDY; NONHUMAN; RAT; URINE OSMOLALITY; UROTHELIAL CELL CULTURE; UROTHELIUM; ANIMAL; CYTOLOGY; EPITHELIUM CELL; METABOLISM; OSMOLARITY; WISTAR RAT;

RATTUS; RATTUS NORVEGICUS;

ANIMALS; ANNEXIN A5; APOPTOSIS; CELLS, CULTURED; CYCLOPHOSPHAMIDE; EPITHELIAL CELLS; FEMALE; FLOW CYTOMETRY; OSMOLAR CONCENTRATION; RATS; RATS, WISTAR;

EID: 84864584276     PISSN: 14258153     EISSN: 16891392     Source Type: Journal    
DOI: 10.2478/s11658-012-0002-y     Document Type: Article

References (33)

1. Martin, S. J., Green, D. R. and Cotter, T. G. Dicing with death: dissecting the components of the apoptosis machinery. Trends. Biochem. Sci. 19 (1994) 26-30.
2. Alberts, K., Johnson, A., Lewis, J., Raff, M. and Roberts, W. P. Programmed cell death eliminates unwanted cells in: Molecular Biology of the Cell (textbook) 5th ed., 2008, Garland Science p. 1115.
3. Ferguson, D. R. Urothelial function. BJU Int. 84 (1999) 235-242.
4. Cockayne, D. A., Hamilton, S. G., Zhu, Q. M., Dunn, P. M., Zhong, Y., Novakovic, S., Malmberg, A. B., Cain, G., Berson, A., Kassotakis, L., Hedley, L., Lachnit, W. G., Burnstock, G., McMahon, S. B. and Ford, A. P. Urinary bladder hyporeflexia and reduced pain-related behaviour in P2X3-deficient mice. Nature407 (2000) 1011-1015.
5. Birder, L. A., Kanai, A. J., de Groat, W. C., Kiss, S., Nealen, M. L., Burke, N. E., Dineley, K. E., Watkins, S., Reynolds, I. J. and Caterina, M. J. Vanilloid receptor expression suggests a sensory role for urinary bladder epithelial cells. Proc. Natl. Acad. Sci. USA98 (2001) 13396-13401.
6. Chopra, B., Barrick, S. R., Meyers, S., Beckel, J. M., Zeidel, M. L., Ford, A. P. D. W., de Groat, W. C. and Birder, L. A. Expression and function of bradykinin B1 and B2 receptors in normal and inflamed rat urinary bladder urothelium. J. Physiol. 562 (2005) 859-871.
7. Szallasi, A. and Blumberg, P. M. Vanilloid (Capsaicin) receptors and mechanisms. Pharmacol. Rev. 51 (1999) 159-212.
8. Zhang, Y. Y., Ludwikowski, B., Hurst, R. and Frey, P. Expansion and longterm culture of differentiated normal rat urothelial cells in vitro. In Vitro Cell. Dev. Biol. Anim. 37 (2001) 419-429.
9. Noguchi, S., Yura, Y., Sherwood, E. R., Kakinuma, H., Kashihara, N. and Oyasu, R. Stimulation of stromal cell growth by normal rat urothelial cell derived epidermal growth factor. Lab. Invest. 62 (1990) 538-544.
10. Garland, A., Jordan, J. E., Necheles, J., Alger, L. E., Scully, M. M., Miller, R. J., Ray, D. W., White, S. R. and Solway, J. Hypertonicity, but not hypothermia, elicits substance P release from rat C-fiber neurons in primary culture. J. Clin. Invest. 95 (1995) 2359-2366.
11. Juszczak, K., Krolczyk, G., Filipek, M., Dobrowolski, Z. F. and Thor, P. J. Animal models of overactive bladder: cyclophosphamide (CYP)-induced cystitis in rats. Fol. Med. Cracov48 (2007) 113-123.
12. Juszczak, K., Ziomber, A., Wyczółkowski, M. and Thor, P. J. Hyperosmolarity alters the micturition: The comparison of urinary bladder motor activity in hyperosmolar and cyclophosphamide-induced models of overactive bladder. Can. J. Physiol. Pharmacol. 88 (2010) 899-906.
13. Kulick, L. J., Clemons, D. J., Hall, R. L. and Koch, M. A. Refinement of the urine concentration test in rats. Contemp. Top. Lab. Anim. Sci. 44 (2005) 46-49.
14. Birder, L. A., Apodaca, G., de Groat, W. C. and Kanai, A. J. Adrenergic- and capsaicin-evoked nitric oxide release from urothelium and afferent nerves in urinary bladder. Am. J. Physiol. Renal. Physiol. 275 (1998) F226-F229.
15. de Groat, W. C. The urothelium in overactive bladder: passive bystander or active participant? Urology64 (2004) 7-11.
16. Sun, Y., Keay, S., DeDeyne, P. and Chai, T. C. Augmented stretch activated adenosine triphosphate release from bladder uroepithelial cells in patients with interstitial cystitis. J. Urol. 166 (2001) 1951-1956.
17. Birder, L. A., Kanai, A. J., de Groat, W. C., Kiss, S., Nealen, M. L., Burke, N. E., Dineley, K. E., Watkins, S., Reynolds, I. J. and Caterina, M. J. Vanilloid receptor expression suggests a sensory role for urinary bladder epithelial cells. Proc. Natl. Acad. Sci. USA98 (2001) 13396-13401.
18. Lewczyński, D. and Ginalski, K. The interactome: predicting the proteinprotein interactions in cells. Cell. Mol. Biol. Lett. 14 (2009) 1-22.
19. Rolius, R., Antoniou, C., Nazarova, L. A., Kim, S. H., Cobb, G., Gala, P., Rajaram, P., Li, Q. and Fung, L. W. M. Inhibition of calpain but not caspase activity by spectrin fragments. Cell. Mol. Biol. Lett. 15 (2010) 395-405.
20. Urbanova, M., Plzak, J., Strnad, H. and Betka, J. Circulating nucleic acids as a new diagnostic tool. Cell. Mol. Biol. Lett. 15 (2010) 242-259.
21. Philips, F. S., Sternberg, S. S., Cronin, A. P. and Vidal, P. M. Cyclophosphamide and urinary bladder toxicity. Cancer Res. 21 (1961) 1577-1589.
22. Locher, G. W. and Cooper, E. H. Repair of rat urinary bladder epithelium following injury by cyclophosphamide. Invest. Urol. 8 (1970) 116-123.
23. Romih, R., Koprivec, D., Martinic, D. S. and Jezernik, K. Restoration of the rat urothelium after cyclophosphamide treatment. Cell. Biol. Int. 25 (2001) 531-537.
24. Wyllie, A. H., Kerr, J. F. R. and Currie, A. R. Cell death: the significance of apoptosis. Int. Rev. Cytol. 68 (1980) 251-306.
25. Duvall, E. and Wyllie, A. H. Death and the cell. Immunol. Today7 (1986) 115-119.
26. Arends, M. J. and Wyllie, A. H. Apoptosis: mechanisms and roles in pathology. Int. Rev. Exp. Pathol. 32 (1991) 223-254.
27. Kullmann, F. A., Shah, M. A., Birder, L. A. and de Groat, W. C. Functional TRP and ASIC-like channels in cultured urothelial cells from the rat. Am. J. Physiol. Renal. Physiol. 296 (2009) F892-F901.
28. Juszczak, K., Ziomber, A., Wyczółkowski, M. and Thor, P. J. Urodynamic effects of the bladder C-fiber afferent activity modulation in chronic overactive bladder model rats. J. Physiol. Pharmacol. 60 (2009) 85-91.
29. Juszczak, K., Gil, K., Wyczółkowski, M. and Thor, P. J. Functional, histological structure and mastocytes numbers alterations in rat urinary bladders following acute and chronic cyclophosphamide treatment. J. Physiol. Pharmacol. 61 (2010) 477-482.
30. Juszczak, K., Wyczółkowski, M. and Thor, P. J. Urodynamic evaluation of experimental rat models of urinary bladder dysfunction: a systematic review of the literature. Ann. Urol. 1 (2011) 1-13.
31. Geppetti, P., Nassini, R., Materazzi, S. and Benemei, S. The concept of neurogenic inflammation. BJU Int. 101Suppl. 3 (2008) 2-6.
32. Gauruder-Burmester, A. and Popken, G. Follow-up at 24 months after treatment of overactive bladder with 0. 2% sodium chondroitin sulphate. Aktuelle Urol. 40 (2009) 355-359.
33. Yeh, C. H., Chiang, H. S. and Chien, C. T. Hyaluronic acid ameliorates bladder hyperactivity via the inhibition of H2O2-enhanced purinergic and muscarinic signalling in the rat. Neurourol. Urodyn. 29 (2010) 765-770.