A new inducible expression system in a transformed green alga, Chlorella vulgaris

Genetics and Molecular Research

Volumn 10, Issue 4, 2011, Pages 3427-3434

  Niu, Y F ^a   Zhang, M H ^a   Xie, W H ^a   Li, J N ^a   Gao, Y F ^a   Yang, W D ^a   Liu, J S ^a   Li, H Y ^a  

^a JINAN UNIVERSITY   (China)

Author keywords

Electroporation; Gene transfer; Inducible promoter; Microalga; Nitrate reductase

Indexed keywords

AMMONIA; CHLORAMPHENICOL; CHLORAMPHENICOL ACETYLTRANSFERASE; NITRATE; NITRATE REDUCTASE;

ALGAL GROWTH; ARTICLE; CHLORELLA VULGARIS; CONTROLLED STUDY; ELECTROPORATION; GENE EXPRESSION; GENE EXPRESSION SYSTEM; GENETIC TRANSFORMATION; GENOME; NITRATE REDUCTASE GENE; NONHUMAN; PROMOTER REGION; REPORTER GENE; TRANSGENIC ORGANISM; ANTIBIOTIC RESISTANCE; DRUG EFFECT; ENZYMOLOGY; GENE VECTOR; GENETIC SELECTION; GENETICS; POLYMERASE CHAIN REACTION;

CHLORAMPHENICOL; CHLORAMPHENICOL O-ACETYLTRANSFERASE; CHLORAMPHENICOL RESISTANCE; CHLORELLA VULGARIS; GENE EXPRESSION; GENETIC VECTORS; NITRATE REDUCTASE; NITRATES; ORGANISMS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; SELECTION, GENETIC; TRANSFORMATION, GENETIC;

EID: 84864553656     PISSN: None     EISSN: 16765680     Source Type: Journal    
DOI: 10.4238/2011.October.21.1     Document Type: Article

References (11)

1. Apt KE, Kroth-Pancic PG and Grossman AR (1996). Stable nuclear transformation of the diatom Phaeodactylum tricornutum. Mol. Gen. Genet. 252: 572-579.
2. Chen Y, Wang Y, Sun Y, Zhang L, et al. (2001). Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells. Curr. Genet. 39: 365-370.
3. del Campo JA, Garcia-Gonzalez M and Guerrero MG (2007). Outdoor cultivation of microalgae for carotenoid production: current state and perspectives. Appl. Microbiol. Biotechnol. 74: 1163-1174.
4. Kim DH, Kim YT, Cho JJ, Bae JH, et al. (2002). Stable integration and functional expression of flounder growth hormone gene in transformed microalga, Chlorella ellipsoidea. Mar. Biotechnol. 4: 63-73.
5. Leon R and Fernandez E (2007). Nuclear transformation of eukaryotic microalgae: historical overview, achievements and problems. Adv. Exp. Med. Biol. 616: 1-11.
6. Loppes R, Radoux M, Ohresser MC and Matagne RF (1999). Transcriptional regulation of the Nia1 gene encoding nitrate reductase in Chlamydomonas reinhardtii: effects of various environmental factors on the expression of a reporter gene under the control of the Nia1 promoter. Plant Mol. Biol. 41: 701-711.
7. Miyagawa A, Okami T, Kira N, Yamaguchi H, et al. (2009). High efficiency transformation of the diatom Phaeodactylum tricornutum with a promoter from the diatom Cylindrotheca fusiformis. Phycol. Res. 57: 142-146.
8. Poulsen N and Kröger N (2005). A new molecular tool for transgenic diatoms: control of mRNA and protein biosynthesis by an inducible promoter-terminator cassette. FEBS J. 272: 3413-3423.
9. Poulsen N, Chesley PM and Kröger N (2006). Molecular genetic manipulation of the diatom Thalassiosira pseudonana (Bacillariophyceae). J. Phycol. 42: 1059-1065.
10. Pruvost J, Van Vooren G, Cogne G and Legrand J (2009). Investigation of biomass and lipids production with Neochloris oleoabundans in photobioreactor. Bioresour. Technol. 100: 5988-5995.
11. Sun Y, Yang Z, Gao X, Li Q, et al. (2005). Expression of foreign genes in Dunaliella by electroporation. Mol. Biotechnol. 30: 185-192.