Imaging synaptic protein dynamics using photoactivatable green fluorescent protein

Cold Spring Harbor Protocols

Volumn 7, Issue 7, 2012, Pages 771-777

  Weimer, Robby M ^a   Hill, Travis C ^a   Hamilton, Andrew M ^a   Zito, Karen ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; NERVE PROTEIN; SYNAPTIC PROTEIN; UNCLASSIFIED DRUG; HYBRID PROTEIN;

ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; DENDRITIC SPINE; FLUORESCENCE MICROSCOPY; HIPPOCAMPUS; NONHUMAN; PHOTOACTIVATION; PHOTOSTIMULATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PYRAMIDAL NERVE CELL; RODENT; SYNAPSE; TIME LAPSE IMAGING; ANIMAL; CHEMISTRY; CYTOLOGY; GENETICS; IMAGE PROCESSING; METABOLISM; METHODOLOGY; NEUROBIOLOGY; PHYSIOLOGY; STAINING;

ANIMALIA; MAMMALIA; RODENTIA;

ANIMALS; CEREBRAL CORTEX; DENDRITIC SPINES; GREEN FLUORESCENT PROTEINS; IMAGE PROCESSING, COMPUTER-ASSISTED; NEUROBIOLOGY; RECOMBINANT FUSION PROTEINS; RODENTIA; STAINING AND LABELING; SYNAPSES;

EID: 84864433596     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot070029     Document Type: Article

References (22)

1. Barretto RP, Messerschmidt B, Schnitzer MJ. 2009. In vivo fluorescence imaging with high-resolution microlenses. Nat Methods 6: 511-512.
2. Bloodgood BL, Sabatini BL. 2005. Neuronal activity regulates diffusion across the neck of dendritic spines. Science 310: 866-869.
3. Gray NW, Weimer RM, Bureau I, Svoboda K. 2006. Rapid redistribution of synaptic PSD-95 in the neocortex in vivo. PLoS Biol 4: e370. doi: 10.1371/journalpbio.0040370.
4. Grutzendler J, Kasthuri N, Gan W-B. 2002. Long-term dendritic spine stability in the adult cortex. Nature 420: 812-816.
5. Holtmaat A, de Paola V, Wilbrecht L, Trachtenberg J, Svoboda K, Portera-Cailliau C. 2011. Imaging neocortical neurons through a chronic cranial window. In: Imaging in neuroscience: A laboratory manual (ed F Helmchen, A Konnerth). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (in press).
6. Kuriu T, Inoue A, Bito H, Sobue K, Okabe S. 2006. Differential control of postsynaptic density scaffolds via actin-dependent and-independent mechanisms. J Neurosci 26: 7693-7706.
7. Mainen ZF, Maletic-Savatic M, Shi SH, Hayashi Y, Malinow R, Svoboda K. 1999. Two-photon imaging in living brain slices. Methods 18: 231-239.
8. Majewska A, Yiu G, Yuste R. 2000. A custom-made two-photon microscope and deconvolution system. Pflugers Arch 441: 398-408.
9. Mizrahi A, Crowley JC, Shtoyerman E, Katz LC. 2004. High-resolution in vivo imaging of hippocampal dendrites and spines. J Neurosci 24: 3147-3151.
10. Niwa H, Yamamura K, Miyazaki J. 1991. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108: 193-199.
11. Patterson GH, Lippincott-Schwartz J. 2002. A photoactivatable GFP for selective photolabeling of proteins and cells. Science 297: 1873-1877.
12. Pologruto TA, Sabatini BL, Svoboda K. 2003. ScanImage: Flexible software for operating laser scanning microscopes. Biomed Eng Online 2: 13. doi: 10.1186/1475-925X-2-13.
13. Saito T. 2006. In vivo electroporation in the embryonic mouse central nervous system. Nat Protoc 1: 1552-1558.
14. Sharma K, Fong DK, Craig AM. 2006. Postsynaptic protein mobility in dendritic spines: Long-term regulation by synaptic NMDA receptor activation. Mol Cell Neurosci 31: 702-712.
15. Sturgill JF, Steiner P, Czervionke BL, Sabatini BL. 2009. Distinct domains within PSD-95 mediate synaptic incorporation, stabilization, and activity-dependent trafficking. J Neurosci 29: 12845-12854.
16. Svoboda K, Yasuda R. 2006. Principles of two-photon excitation microscopy and its applications to neuroscience. Neuron 50: 823-839.
17. Trachtenberg JT, Chen BE, Knott GW, Feng G, Sanes JR, Welker E, Svoboda K. 2002. Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex. Nature 420: 788-794.
18. Tsuriel S, Geva R, Zamorano P, Dresbach T, Boeckers T, Gundelfinger ED, Garner CC, Ziv NE. 2006. Local sharing as a predominant determinant of synaptic matrix molecular dynamics. PLoS Biol 4: e271. doi: 10.1371/journal. pbio.0040271.
19. Woods G, Zito K. 2008. Preparation of gene gun bullets and biolistic transfection of neurons in slice culture. J Vis Exp 12. http://www.jove.com/index/Details.stp?ID=675, doi: 10.3791/675.
20. Xu W, Schlüter OM, Steiner P, Czervionke BL, Sabatini B, Malenka RC. 2008. Molecular dissociation of the role of PSD-95 in regulating synaptic strength and LTD. Neuron 57: 248-262.
21. Zito K, Knott G, Shepherd GM, Shenolikar S, Svoboda K. 2004. Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton. Neuron 44: 321-334.
22. Zito K, Scheuss V, Knott G, Hill T, Svoboda K. 2009. Rapid functional maturation of nascent dendritic spines. Neuron 61: 247-258.