Proteomic analysis of the impact of static culturing on the expansion of rat bone marrow mesenchymal stem cells

Biotechnology Letters

Volumn 34, Issue 8, 2012, Pages 1589-1596

  Peter, Stefan ^a   Evans, Caroline ^b   Ow, Saw Y ^b   Scutt, Andy M ^b   Wright, Phillip C ^b   Biggs, Catherine A ^b  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Ageing; iTRAQ; Mesenchymal stem cells; Proteomics; Static cultures

Indexed keywords

AGEING; ITRAQ; MESENCHYMAL STEM CELL; PROTEOMICS; STATIC CULTURE;

CELL CULTURE; MOLECULAR BIOLOGY; MONOLAYERS; RATS; STEM CELLS; TISSUE ENGINEERING;

PROTEINS;

BIOLOGICAL MARKER; PROTEOME;

ANIMAL; ARTICLE; BONE DEVELOPMENT; BONE MARROW CELL; CELL AGING; CELL DIFFERENTIATION; CELL PROLIFERATION; CULTURE TECHNIQUE; ISOTOPE LABELING; MESENCHYMAL STROMA CELL; METABOLISM; METHODOLOGY; PHENOTYPE; PROTEOMICS; RAT;

ANIMALS; BIOLOGICAL MARKERS; BONE MARROW CELLS; CELL AGING; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; ISOTOPE LABELING; MESENCHYMAL STROMAL CELLS; OSTEOGENESIS; PHENOTYPE; PROTEOME; PROTEOMICS; RATS;

RATTUS;

EID: 84864394080     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-012-0935-2     Document Type: Article

References (20)

1. Boehm A, Putz S, Altenhofer D, Sickmann A, Falk M (2007) Precise protein quantification based on peptide quantification using iTRAQTM. BMC Bioinformatics 8: 214-232.
2. Bruder SP, Jaiswal N, Haynesworth SE (1997) Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive sub-cultivation and following cryopreservation. J Cell Biochem 64: 278-294.
3. Celebi BL, Elcin YM (2009) Proteome analysis of rat bone marrow mesenchymal stem cell subcultures. J Proteome Res 8: 2164-2172.
4. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj, Horwitz E (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy 8: 315-317.
5. Glen A, Gan CS, Hamdy FC, Eaton CL, Cross SS, Catto JW, Wright PC, Rehman I (2008) iTRAQ-facilitated proteomic analysis of human prostate cancer cells identifies proteins associated with progression. J Proteome Res 7: 897-907.
6. Huang DW, Sherman BT, Lempicki RA (2008) Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc 4: 44-57.
7. Huang DW, Sherman BT, Lempicki RA (2009) Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists. Nucleic Acid Res 37: 1-13.
8. Kim JS, Lee HK, Kim MR, Kim PK, Kim CW (2008) Differentially expressed proteins of mesenchymal stem cells derived from human umbilical cord blood during osteogenic differentiation. Biosci Biotechnol Biochem 72: 2309-2317.
9. Lee HK, Lee BH, Park SA, Kim CW (2006) The proteomic analysis of an adipocyte differentiated from human mesenchymal stem cells using two-dimensional gel electrophoresis. Proteomics 6: 1223-1229.
10. Maurer MH, Kuschinsky WK (2007) Proteomics. In: Lajtha A, Gibson GE, Dienel GA (eds) Handbook of neurochemistry and molecular neurobiology, vol 5., brain energetics. Integration of cellular and molecular processesSpringer, New York, pp 737-769.
11. Noirel J, Evans C, Salim M, Mukherjee J, Yen OS, Pandhal J, Pham TK, Biggs CA, Wright PC (2011) Methods in quantitative proteomics: setting iTRAQ on the right track. Curr Proteomics 8: 17-30.
12. Park HW, Shin JS, Kim CW (2007) Proteome of mesenchymal stem cells. Proteomics 7: 2881-2894.
13. Pham TK, Roy S, Noirel J, Douglas I, Wright PC, Stafford GP (2010) A quantitative proteomic analysis of biofilm adaptation by the periodontal pathogen Tannerella forsythia. Proteomics 10: 3130-3141.
14. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284: 143-147.
15. Scutt A, Reading L, Scutt N, Still K (2003) Mineralizing fibroblast-colony-forming assays. Methods Mol Med 80: 29-39.
16. Stolzing A, Scutt A (2006) Age-related impairment of mesenchymal progenitor cell function. Ageing Cell 5: 213-224.
17. Sun HJ, Bahk YY, Choi YR, Shim JH, Han SH, Lee JW (2006) A proteomic analysis during serial subculture and osteogenic differentiation of human mesenchymal stem cell. J Orthop Res 24: 2059-2071.
18. Yudin D, Fainzilber M (2009) RAN on tracks--cytoplasmic roles for a nuclear regulator. J Cell Sci 122: 587-593.
19. Zhang Y, Wolf-Yadlin A, Ross PL, Pappin DJ, Rush J, Lauffenburger DA, White FM et al (2005) Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules. Mol Cell Proteomics 4: 1240-1250.
20. Zhang AX, Yu WH, Ma BF, Yu XB, Mao FF, Liu W, Zhang JQ, Zhang XM, Li SN, Li MT, Lahn BT, Xiang AP (2007) Proteomic identification of differently expressed proteins responsible for osteoblast differentiation from human mesenchymal stem cells. Mol Cell Biochem 304: 167-179.